Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum
Fusarium wilt is a plant disease caused by Fusarium oxysporum that generates significant economic loss to crops. A method to sustainably control F. oxysporum is utilizing biological agents, such as endophytic bacteria. Therefore, this study aimed to isolate endophytic bacteria from tomato plant tiss...
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| Format: | Article |
| Language: | English |
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Universitas Sebelas Maret, Faculty of Agriculture
2022-12-01
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| Series: | Caraka Tani: Journal of Sustainable Agriculture |
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| Online Access: | https://jurnal.uns.ac.id/carakatani/article/view/64667 |
| _version_ | 1797703617651671040 |
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| author | Ratna Umi Nurlila Jumarddin La Fua Muhammad Sainal Abidin Sulaeha Sulaeha Lodes Hadju |
| author_facet | Ratna Umi Nurlila Jumarddin La Fua Muhammad Sainal Abidin Sulaeha Sulaeha Lodes Hadju |
| author_sort | Ratna Umi Nurlila |
| collection | DOAJ |
| description | Fusarium wilt is a plant disease caused by Fusarium oxysporum that generates significant economic loss to crops. A method to sustainably control F. oxysporum is utilizing biological agents, such as endophytic bacteria. Therefore, this study aimed to isolate endophytic bacteria from tomato plant tissue, which could inhibit the pathogen of wilt disease (F. oxysporum). Endophytic bacteria were isolated from local tomato plants in Muna Regency, Indonesia. Morphological characteristics such as size, shape, color and height of bacterial colonies were then determined by Gram staining using potassium hydroxide (KOH). Endophytic bacterial isolates were evaluated for their ability to inhibit F. oxysporum through inhibition and hydrogen cyanide (HCN) production tests. Subsequently, analysis of variance was used to determine whether endophytic bacteria inhibited F. oxysporum growth, and if there was a significant effect, Duncan's test was conducted at 95% significance. HCN production was observed through qualitative methods. The results showed that four endophytic bacteria isolates, namely LBR I A03, SWR II B04, SDM II B05 and SWR I A02 inhibited the growth of F. oxysporum by more than 50%. It also revealed that four endophytic bacterial isolates were strong HCN producers and two were weak producers. Therefore, isolates showing antifungal activity in this study can be used as biopesticide agents to induce plant resistance to F. oxysporum. |
| first_indexed | 2024-03-12T05:07:14Z |
| format | Article |
| id | doaj.art-eb12662d6cb84b9bbc48d46e4860f470 |
| institution | Directory Open Access Journal |
| issn | 2613-9456 2599-2570 |
| language | English |
| last_indexed | 2024-03-12T05:07:14Z |
| publishDate | 2022-12-01 |
| publisher | Universitas Sebelas Maret, Faculty of Agriculture |
| record_format | Article |
| series | Caraka Tani: Journal of Sustainable Agriculture |
| spelling | doaj.art-eb12662d6cb84b9bbc48d46e4860f4702023-09-03T08:54:08ZengUniversitas Sebelas Maret, Faculty of AgricultureCaraka Tani: Journal of Sustainable Agriculture2613-94562599-25702022-12-01381536410.20961/carakatani.v38i1.6466736386Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporumRatna Umi Nurlila0Jumarddin La Fua1Muhammad Sainal Abidin2Sulaeha Sulaeha3Lodes Hadju4Department of Pharmacy, Faculty of Science and Technology, Universitas Mandala Waluya, KendariDepartment of Tadris Biology, Institut Agama Islam Negeri (IAIN), KendariDepartment of Physics, Faculty of Science and Technology, Universitas Mandala Waluya, KendariDepartment of Plant Protection, Faculty of Agriculture, Hasanuddin University, MakassarDepartment of Health, Faculty of Public Health, Lincoln University College, Kota BharuFusarium wilt is a plant disease caused by Fusarium oxysporum that generates significant economic loss to crops. A method to sustainably control F. oxysporum is utilizing biological agents, such as endophytic bacteria. Therefore, this study aimed to isolate endophytic bacteria from tomato plant tissue, which could inhibit the pathogen of wilt disease (F. oxysporum). Endophytic bacteria were isolated from local tomato plants in Muna Regency, Indonesia. Morphological characteristics such as size, shape, color and height of bacterial colonies were then determined by Gram staining using potassium hydroxide (KOH). Endophytic bacterial isolates were evaluated for their ability to inhibit F. oxysporum through inhibition and hydrogen cyanide (HCN) production tests. Subsequently, analysis of variance was used to determine whether endophytic bacteria inhibited F. oxysporum growth, and if there was a significant effect, Duncan's test was conducted at 95% significance. HCN production was observed through qualitative methods. The results showed that four endophytic bacteria isolates, namely LBR I A03, SWR II B04, SDM II B05 and SWR I A02 inhibited the growth of F. oxysporum by more than 50%. It also revealed that four endophytic bacterial isolates were strong HCN producers and two were weak producers. Therefore, isolates showing antifungal activity in this study can be used as biopesticide agents to induce plant resistance to F. oxysporum.https://jurnal.uns.ac.id/carakatani/article/view/64667antagonistic bacteriabiocontrolbiopesticideendophyteshcn production |
| spellingShingle | Ratna Umi Nurlila Jumarddin La Fua Muhammad Sainal Abidin Sulaeha Sulaeha Lodes Hadju Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum Caraka Tani: Journal of Sustainable Agriculture antagonistic bacteria biocontrol biopesticide endophytes hcn production |
| title | Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum |
| title_full | Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum |
| title_fullStr | Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum |
| title_full_unstemmed | Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum |
| title_short | Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum |
| title_sort | effectiveness of endophytic bacteria from local tomato plants against wilt disease caused by fusarium oxysporum |
| topic | antagonistic bacteria biocontrol biopesticide endophytes hcn production |
| url | https://jurnal.uns.ac.id/carakatani/article/view/64667 |
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