Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection
Every human body is a source of a unique scent, which can be used for medical or forensic purposes. Human skin scent is a complex mixture of more or less volatile compounds with different chemical and physical properties, which often differ significantly in their concentrations. The most efficient t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/8/12/232 |
| _version_ | 1797500608042762240 |
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| author | Petra Pojmanová Nikola Ladislavová Štěpán Urban |
| author_facet | Petra Pojmanová Nikola Ladislavová Štěpán Urban |
| author_sort | Petra Pojmanová |
| collection | DOAJ |
| description | Every human body is a source of a unique scent, which can be used for medical or forensic purposes. Human skin scent is a complex mixture of more or less volatile compounds with different chemical and physical properties, which often differ significantly in their concentrations. The most efficient technique for separating such complex samples is comprehensive two-dimensional gas chromatography (GC × GC). This work aimed to find the optimal arrangement of a two-dimensional chromatographic system and define a suitable chromatographic method for non-targeted analysis of human scent samples. Four different chromatographic columns (non-polar Rxi-5MS and TG-5HT, medium polar Rxi-17Sil MS and Rtx-200MS) and their different configurations were tested. The best system was the 30 m primary column Rtx-200MS (with the 2 m pre-column Rtx-200MS) and the 1 m secondary column TG-5HT in a reverse configuration. This system achieved the highest theoretical and conditional peak capacities, optimal resolution, and the lowest number of coelutions. |
| first_indexed | 2024-03-10T03:06:16Z |
| format | Article |
| id | doaj.art-eb142040aae44b5a97c2c0abed25a903 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T03:06:16Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-eb142040aae44b5a97c2c0abed25a9032023-11-23T10:32:34ZengMDPI AGSeparations2297-87392021-12-0181223210.3390/separations8120232Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass DetectionPetra Pojmanová0Nikola Ladislavová1Štěpán Urban2Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech RepublicEvery human body is a source of a unique scent, which can be used for medical or forensic purposes. Human skin scent is a complex mixture of more or less volatile compounds with different chemical and physical properties, which often differ significantly in their concentrations. The most efficient technique for separating such complex samples is comprehensive two-dimensional gas chromatography (GC × GC). This work aimed to find the optimal arrangement of a two-dimensional chromatographic system and define a suitable chromatographic method for non-targeted analysis of human scent samples. Four different chromatographic columns (non-polar Rxi-5MS and TG-5HT, medium polar Rxi-17Sil MS and Rtx-200MS) and their different configurations were tested. The best system was the 30 m primary column Rtx-200MS (with the 2 m pre-column Rtx-200MS) and the 1 m secondary column TG-5HT in a reverse configuration. This system achieved the highest theoretical and conditional peak capacities, optimal resolution, and the lowest number of coelutions.https://www.mdpi.com/2297-8739/8/12/232human skin scentcomprehensive two-dimensional gas chromatographymass spectrometry |
| spellingShingle | Petra Pojmanová Nikola Ladislavová Štěpán Urban Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection Separations human skin scent comprehensive two-dimensional gas chromatography mass spectrometry |
| title | Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection |
| title_full | Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection |
| title_fullStr | Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection |
| title_full_unstemmed | Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection |
| title_short | Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection |
| title_sort | development of a method for the measurement of human scent samples using comprehensive two dimensional gas chromatography with mass detection |
| topic | human skin scent comprehensive two-dimensional gas chromatography mass spectrometry |
| url | https://www.mdpi.com/2297-8739/8/12/232 |
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