Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates
Introduction: Microbial biopolymers such as polyhydroxyalkanoates (PHA) are proper alternatives for petroleum-derived plastics. These biopolymers have many advantages over conventional plastics such as biodegradability, environmental friendly and infinite as a renewable resource. Therefore, our stud...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2017-12-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://bjm.ui.ac.ir/article_21697_df0cfb6fd7ed4dbd00b446dd2ad387cb.pdf |
| _version_ | 1818997481559556096 |
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| author | Nazila Gholamveisi Sirvan Mohammadi Azar Roya Moravej |
| author_facet | Nazila Gholamveisi Sirvan Mohammadi Azar Roya Moravej |
| author_sort | Nazila Gholamveisi |
| collection | DOAJ |
| description | Introduction: Microbial biopolymers such as polyhydroxyalkanoates (PHA) are proper alternatives for petroleum-derived plastics. These biopolymers have many advantages over conventional plastics such as biodegradability, environmental friendly and infinite as a renewable resource. Therefore, our study was aimed to isolate a bacterial strain capable of producing polyhydroxybutyrate (PHB; a highly applicable type of PHA). Materials and methods: To this aim, a total of 6 PHA-producing bacteria were isolated from waste water exit site of a brewery factory. The 6 isolates were studied by Sudan black-staining technique and the most stained isolate was selected for further studies. Next, the selected isolate was identified based on morphological, biochemical and phylogenetic analyses. Finally, the ability of strain in producing PHB as well as other PHAs was analyzed via GC-MS technique. Results: Strain NG had the highest yield of PHB, according to Sudan black-staining technique and it was selected for further studies. The strain NG was identified as a new strain of Bacillus thuringiensis. According to GC-MS results, this strain was able to produce PHB as well as 4 other PHAs including hexadecanoic acid methyl ester, octadecanoic acid methyl ester, tetradecanoic acid methyl ester, 8-octadecenoic acid methyl ester. Discussion and conclusion: It was the first report on producing various PHAs at the same time by a strain of Bacillus thuringiensis. |
| first_indexed | 2024-12-20T21:46:19Z |
| format | Article |
| id | doaj.art-eb15ffbb873549e3989ae4b7a113fe2b |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-20T21:46:19Z |
| publishDate | 2017-12-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-eb15ffbb873549e3989ae4b7a113fe2b2022-12-21T19:25:40ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812017-12-01624132010.22108/bjm.2017.2169721697Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various PolyhydroxyalkanoatesNazila Gholamveisi0Sirvan Mohammadi Azar1Roya Moravej2M.Sc Student of Microbiology, Department of Biology, College of Basic Sciences, Islamic Azad University of Sanandaj, IranAssistant Professor of Chemistry, Department of Chemistry, College of Basic Sciences, Islamic Azad University of Sanandaj, IranInstructor of Microbiology, Department of Biology, Faculty of Science, Islamic Azad University of Sanandaj, IranIntroduction: Microbial biopolymers such as polyhydroxyalkanoates (PHA) are proper alternatives for petroleum-derived plastics. These biopolymers have many advantages over conventional plastics such as biodegradability, environmental friendly and infinite as a renewable resource. Therefore, our study was aimed to isolate a bacterial strain capable of producing polyhydroxybutyrate (PHB; a highly applicable type of PHA). Materials and methods: To this aim, a total of 6 PHA-producing bacteria were isolated from waste water exit site of a brewery factory. The 6 isolates were studied by Sudan black-staining technique and the most stained isolate was selected for further studies. Next, the selected isolate was identified based on morphological, biochemical and phylogenetic analyses. Finally, the ability of strain in producing PHB as well as other PHAs was analyzed via GC-MS technique. Results: Strain NG had the highest yield of PHB, according to Sudan black-staining technique and it was selected for further studies. The strain NG was identified as a new strain of Bacillus thuringiensis. According to GC-MS results, this strain was able to produce PHB as well as 4 other PHAs including hexadecanoic acid methyl ester, octadecanoic acid methyl ester, tetradecanoic acid methyl ester, 8-octadecenoic acid methyl ester. Discussion and conclusion: It was the first report on producing various PHAs at the same time by a strain of Bacillus thuringiensis.http://bjm.ui.ac.ir/article_21697_df0cfb6fd7ed4dbd00b446dd2ad387cb.pdfBacillus thuringiensis NGPolyhydroxyalkanoatespolyhydroxybutyrateGC-MS |
| spellingShingle | Nazila Gholamveisi Sirvan Mohammadi Azar Roya Moravej Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates Biological Journal of Microorganism Bacillus thuringiensis NG Polyhydroxyalkanoates polyhydroxybutyrate GC-MS |
| title | Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates |
| title_full | Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates |
| title_fullStr | Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates |
| title_full_unstemmed | Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates |
| title_short | Bacillus thuringiensis strain NG, a Novel Isolated Strain for production of Various Polyhydroxyalkanoates |
| title_sort | bacillus thuringiensis strain ng a novel isolated strain for production of various polyhydroxyalkanoates |
| topic | Bacillus thuringiensis NG Polyhydroxyalkanoates polyhydroxybutyrate GC-MS |
| url | http://bjm.ui.ac.ir/article_21697_df0cfb6fd7ed4dbd00b446dd2ad387cb.pdf |
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