Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens
The present article describes the scientific and methodological development of biotechnological manufacture of test-system components (diagnostic preparations) for instant diagnosis of plague, brucellosis, tularemia, anthrax, cholera. In this regard, in the first place the effective methods for obta...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»
2018-02-01
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| Series: | Биопрепараты: Профилактика, диагностика, лечение |
| Subjects: | |
| Online Access: | https://www.biopreparations.ru/jour/article/view/29 |
| _version_ | 1826566493975871488 |
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| author | I. S. Tyumentseva I. V. Zharnikova E. N. Afanasiev V. I. Efremenko O. I. Kogotkova S. M. Kalnoy A. N. Kulichenko |
| author_facet | I. S. Tyumentseva I. V. Zharnikova E. N. Afanasiev V. I. Efremenko O. I. Kogotkova S. M. Kalnoy A. N. Kulichenko |
| author_sort | I. S. Tyumentseva |
| collection | DOAJ |
| description | The present article describes the scientific and methodological development of biotechnological manufacture of test-system components (diagnostic preparations) for instant diagnosis of plague, brucellosis, tularemia, anthrax, cholera. In this regard, in the first place the effective methods for obtaining complete antigenic complexes used for immunizing animals for the purpose of developing highly potent immune sera have been established. These antisera were used in determining optimum parameters of manufacture on the basis of their diagnosticums. Methodical basis of developing magnetic immunosorbents for selective concentration of infectious agents and their instant diagnosis methods has been mentioned. Moreover, the article describes the development of piezoelectric quartz crystal biosensors to detect plague, brucellosis and tularemia pathogens by gravimetric flow injection analysis, allowing to quickly implement the process of reliable identification of a test pathogen in antigen-antibody complex. |
| first_indexed | 2024-03-08T21:44:34Z |
| format | Article |
| id | doaj.art-eb1709f1ca364edeaf29f986b59bde2a |
| institution | Directory Open Access Journal |
| issn | 2221-996X 2619-1156 |
| language | Russian |
| last_indexed | 2025-03-14T10:52:32Z |
| publishDate | 2018-02-01 |
| publisher | Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» |
| record_format | Article |
| series | Биопрепараты: Профилактика, диагностика, лечение |
| spelling | doaj.art-eb1709f1ca364edeaf29f986b59bde2a2025-03-02T10:49:19ZrusMinistry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»Биопрепараты: Профилактика, диагностика, лечение2221-996X2619-11562018-02-0104212529Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogensI. S. Tyumentseva0I. V. Zharnikova1E. N. Afanasiev2V. I. Efremenko3O. I. Kogotkova4S. M. Kalnoy5A. N. Kulichenko6Stavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareStavropol Research Anti-Plaque Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human WelfareThe present article describes the scientific and methodological development of biotechnological manufacture of test-system components (diagnostic preparations) for instant diagnosis of plague, brucellosis, tularemia, anthrax, cholera. In this regard, in the first place the effective methods for obtaining complete antigenic complexes used for immunizing animals for the purpose of developing highly potent immune sera have been established. These antisera were used in determining optimum parameters of manufacture on the basis of their diagnosticums. Methodical basis of developing magnetic immunosorbents for selective concentration of infectious agents and their instant diagnosis methods has been mentioned. Moreover, the article describes the development of piezoelectric quartz crystal biosensors to detect plague, brucellosis and tularemia pathogens by gravimetric flow injection analysis, allowing to quickly implement the process of reliable identification of a test pathogen in antigen-antibody complex.https://www.biopreparations.ru/jour/article/view/29чумабруцеллезтуляремиясибирская язвахолераантигениммунная сыворотка кровиреакция непрямой гемагглютинациииммуноферментный анализполимеразная цепная реакциямагноиммуносорбентпьезокварцевые биосенсорные устройстваplaguebrucellosistularemiaanthraxcholeraantigenimmune serumindirect hemagglutination reactionimmunosorbent assaypolymerase chain reactionmagnesium immunosorbentpiezoelectric quartz crystal biosensor |
| spellingShingle | I. S. Tyumentseva I. V. Zharnikova E. N. Afanasiev V. I. Efremenko O. I. Kogotkova S. M. Kalnoy A. N. Kulichenko Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens Биопрепараты: Профилактика, диагностика, лечение чума бруцеллез туляремия сибирская язва холера антиген иммунная сыворотка крови реакция непрямой гемагглютинации иммуноферментный анализ полимеразная цепная реакция магноиммуносорбент пьезокварцевые биосенсорные устройства plague brucellosis tularemia anthrax cholera antigen immune serum indirect hemagglutination reaction immunosorbent assay polymerase chain reaction magnesium immunosorbent piezoelectric quartz crystal biosensor |
| title | Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| title_full | Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| title_fullStr | Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| title_full_unstemmed | Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| title_short | Scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| title_sort | scientific and methodical development of biotechnological production of immunobiological preparations for instant diagnosis of infectious diseases and detection of pathogens |
| topic | чума бруцеллез туляремия сибирская язва холера антиген иммунная сыворотка крови реакция непрямой гемагглютинации иммуноферментный анализ полимеразная цепная реакция магноиммуносорбент пьезокварцевые биосенсорные устройства plague brucellosis tularemia anthrax cholera antigen immune serum indirect hemagglutination reaction immunosorbent assay polymerase chain reaction magnesium immunosorbent piezoelectric quartz crystal biosensor |
| url | https://www.biopreparations.ru/jour/article/view/29 |
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