Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery
AIM: To evaluate human lens epithelium cell apoptosis and epithelial to mesenchymal transition (EMT) induced by femtosecond laser in femtosecond laser assisted cataract surgery (FLACS). METHODS: Sixty cataract patients with N2 to N3 stage according to the LOCS III were enrolled in this study and di...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-03-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2018/3/20180309.pdf |
| _version_ | 1819264889627082752 |
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| author | Wei Sun Jia Liu Jing Li Di Wu Jing Wang Ming-Wu Wang Jin-Song Zhang Jiang-Yue Zhao |
| author_facet | Wei Sun Jia Liu Jing Li Di Wu Jing Wang Ming-Wu Wang Jin-Song Zhang Jiang-Yue Zhao |
| author_sort | Wei Sun |
| collection | DOAJ |
| description | AIM: To evaluate human lens epithelium cell apoptosis and epithelial to mesenchymal transition (EMT) induced by femtosecond laser in femtosecond laser assisted cataract surgery (FLACS).
METHODS: Sixty cataract patients with N2 to N3 stage according to the LOCS III were enrolled in this study and divided into three groups randomly: FLACS1 group (cataract surgery by FLACS with LenSx), FLACS2 group (cataract surgery by FLACS with LensAR) and manual group (cataract surgery by phacoemulsification). Patients in two FLACS groups performed anterior capsulotomy by LenSx or LensAR laser system. Patients in the manual group were performed continuous curvilinear capsulorrhexis (CCC) manually. The anterior capsules were fixed right after moved out of eye. Hematoxylin-eosine staining, immunofluorescence staining and real-time PCR were performed in order to observe human lens epithelium cells changes after cataract surgery.
RESULTS: The capsule cutting edge was shown irregularity and roughness in two FLACS groups and smooth edge in manual capsulotomy by pathologic staining. Irregularities of the cell configuration with partly swollen and destroyed nuclei were observed in two FLACS groups. Femtosecond laser could induce a significantly higher cell apoptosis in human lens epithelium cell than manually performed CCC (P<0.05). Lens epithelium cells apoptosis were correlated with femtosecond laser duration according to Pearson correlation analysis. Decreased N-cadherin expression, alpha-SMA and FSP-1 level in two FLACS groups showed the inhibition of cell EMT.
CONCLUSION: Femtosecond laser may affect the apoptosis and EMT of lens epithelium cells which are under the peeled central lens capsule. |
| first_indexed | 2024-12-23T20:36:39Z |
| format | Article |
| id | doaj.art-eb17f1adb6d54b1086a1f87cb7abc9ae |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-23T20:36:39Z |
| publishDate | 2018-03-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-eb17f1adb6d54b1086a1f87cb7abc9ae2022-12-21T17:32:04ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-03-0111340140710.18240/ijo.2018.03.09Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgeryWei Sun0Jia Liu1Jing Li2Di Wu3Jing Wang4Ming-Wu Wang5Jin-Song Zhang6Jiang-Yue Zhao7Department of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityDepartment of Ophthalmology, the 4th Affiliated Hospital of China Medical UniversityAIM: To evaluate human lens epithelium cell apoptosis and epithelial to mesenchymal transition (EMT) induced by femtosecond laser in femtosecond laser assisted cataract surgery (FLACS). METHODS: Sixty cataract patients with N2 to N3 stage according to the LOCS III were enrolled in this study and divided into three groups randomly: FLACS1 group (cataract surgery by FLACS with LenSx), FLACS2 group (cataract surgery by FLACS with LensAR) and manual group (cataract surgery by phacoemulsification). Patients in two FLACS groups performed anterior capsulotomy by LenSx or LensAR laser system. Patients in the manual group were performed continuous curvilinear capsulorrhexis (CCC) manually. The anterior capsules were fixed right after moved out of eye. Hematoxylin-eosine staining, immunofluorescence staining and real-time PCR were performed in order to observe human lens epithelium cells changes after cataract surgery. RESULTS: The capsule cutting edge was shown irregularity and roughness in two FLACS groups and smooth edge in manual capsulotomy by pathologic staining. Irregularities of the cell configuration with partly swollen and destroyed nuclei were observed in two FLACS groups. Femtosecond laser could induce a significantly higher cell apoptosis in human lens epithelium cell than manually performed CCC (P<0.05). Lens epithelium cells apoptosis were correlated with femtosecond laser duration according to Pearson correlation analysis. Decreased N-cadherin expression, alpha-SMA and FSP-1 level in two FLACS groups showed the inhibition of cell EMT. CONCLUSION: Femtosecond laser may affect the apoptosis and EMT of lens epithelium cells which are under the peeled central lens capsule.http://www.ijo.cn/en_publish/2018/3/20180309.pdf407femtosecond lasers assisted cataract surgerylens epithelium cellapoptosisepithelial mesenchymal transition |
| spellingShingle | Wei Sun Jia Liu Jing Li Di Wu Jing Wang Ming-Wu Wang Jin-Song Zhang Jiang-Yue Zhao Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery International Journal of Ophthalmology 407 femtosecond lasers assisted cataract surgery lens epithelium cell apoptosis epithelial mesenchymal transition |
| title | Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery |
| title_full | Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery |
| title_fullStr | Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery |
| title_full_unstemmed | Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery |
| title_short | Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery |
| title_sort | human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser assisted cataract surgery |
| topic | 407 femtosecond lasers assisted cataract surgery lens epithelium cell apoptosis epithelial mesenchymal transition |
| url | http://www.ijo.cn/en_publish/2018/3/20180309.pdf |
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