Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis
Endometriosis is a highly prevalent gynecological disease characterized by lesions in different sites. Regulation of specific estrogen pathways may favor the formation of distinct microenvironments and the progression of endometriosis. However, no study has simultaneously evaluated the gene and prot...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.854991/full |
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| author | Katiane de Almeida Da Costa Helena Malvezzi Cristine Dobo Cristine Dobo Rosa Maria Neme Rosa Maria Neme Renée Zon Filippi Renée Zon Filippi Thiago Pinheiro Arrais Aloia Elisa Rampazo Prado Juliana Meola Carla de Azevedo Piccinato Carla de Azevedo Piccinato |
| author_facet | Katiane de Almeida Da Costa Helena Malvezzi Cristine Dobo Cristine Dobo Rosa Maria Neme Rosa Maria Neme Renée Zon Filippi Renée Zon Filippi Thiago Pinheiro Arrais Aloia Elisa Rampazo Prado Juliana Meola Carla de Azevedo Piccinato Carla de Azevedo Piccinato |
| author_sort | Katiane de Almeida Da Costa |
| collection | DOAJ |
| description | Endometriosis is a highly prevalent gynecological disease characterized by lesions in different sites. Regulation of specific estrogen pathways may favor the formation of distinct microenvironments and the progression of endometriosis. However, no study has simultaneously evaluated the gene and protein regulation of the main estrogen-synthesizing enzymes in endometriosis. Thus, our goals were to study the relationship between gene and protein expression of aromatase (CYP19A1 or ARO), steroid sulfatase (STS), and hydroxysteroid 17-beta dehydrogenase (HSD17B1) in superficial (SUP), ovarian (OMA), and deep infiltrating (DIE) endometriotic lesion sites as well as in the eutopic endometrium of patients with (EE) and without (control) endometriosis in the same and large cohort of patients. The site-specific expression of these enzymes within different cells (glandular and stromal components) was also explored. The study included 108 patients surgically diagnosed with endometriosis who provided biopsies of EE and endometriotic lesions and 16 disease-free patients who collected normal endometrium tissue. Our results showed that CYP19A1 was detected in all endometriosis tissues and was in higher levels than in control. Unique patterns of the STS and HSD17B1 levels showed that they were most closely regulated in all tissues, with manifestation at greater levels in DIE compared to the other endometriotic lesion sites, OMA and SUP. Gene and protein expression of ARO, STS, and HSD17B1 occurred at different rates in endometriotic sites or EE. The distinctive levels of these estrogen-synthesizing enzymes in each endometriotic site support the hypothesis of a tissue microenvironment that can both influence and be influenced by the expression of different estrogenic pathways, locally affecting the availability of estrogen needed for maintenance and progression of endometriotic lesions. |
| first_indexed | 2024-12-12T21:13:22Z |
| format | Article |
| id | doaj.art-eb187635b8374a7fbc26395fa65c8a4e |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-12T21:13:22Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-eb187635b8374a7fbc26395fa65c8a4e2022-12-22T00:11:49ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-05-01910.3389/fmolb.2022.854991854991Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in EndometriosisKatiane de Almeida Da Costa0Helena Malvezzi1Cristine Dobo2Cristine Dobo3Rosa Maria Neme4Rosa Maria Neme5Renée Zon Filippi6Renée Zon Filippi7Thiago Pinheiro Arrais Aloia8Elisa Rampazo Prado9Juliana Meola10Carla de Azevedo Piccinato11Carla de Azevedo Piccinato12Hospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilDepartment of Clinical Pathology, Hospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilCentro de Endometriose São Paulo, Av. República Do Líbano, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilDepartment of Clinical Pathology, Hospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilDepartment of Gynaecology & Obstetrics, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, BrazilHospital Israelita Albert Einstein, São Paulo, BrazilDepartment of Gynaecology & Obstetrics, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, BrazilEndometriosis is a highly prevalent gynecological disease characterized by lesions in different sites. Regulation of specific estrogen pathways may favor the formation of distinct microenvironments and the progression of endometriosis. However, no study has simultaneously evaluated the gene and protein regulation of the main estrogen-synthesizing enzymes in endometriosis. Thus, our goals were to study the relationship between gene and protein expression of aromatase (CYP19A1 or ARO), steroid sulfatase (STS), and hydroxysteroid 17-beta dehydrogenase (HSD17B1) in superficial (SUP), ovarian (OMA), and deep infiltrating (DIE) endometriotic lesion sites as well as in the eutopic endometrium of patients with (EE) and without (control) endometriosis in the same and large cohort of patients. The site-specific expression of these enzymes within different cells (glandular and stromal components) was also explored. The study included 108 patients surgically diagnosed with endometriosis who provided biopsies of EE and endometriotic lesions and 16 disease-free patients who collected normal endometrium tissue. Our results showed that CYP19A1 was detected in all endometriosis tissues and was in higher levels than in control. Unique patterns of the STS and HSD17B1 levels showed that they were most closely regulated in all tissues, with manifestation at greater levels in DIE compared to the other endometriotic lesion sites, OMA and SUP. Gene and protein expression of ARO, STS, and HSD17B1 occurred at different rates in endometriotic sites or EE. The distinctive levels of these estrogen-synthesizing enzymes in each endometriotic site support the hypothesis of a tissue microenvironment that can both influence and be influenced by the expression of different estrogenic pathways, locally affecting the availability of estrogen needed for maintenance and progression of endometriotic lesions.https://www.frontiersin.org/articles/10.3389/fmolb.2022.854991/fullaromataseCYP19A1STSHSD17B1estradiolstromal cells |
| spellingShingle | Katiane de Almeida Da Costa Helena Malvezzi Cristine Dobo Cristine Dobo Rosa Maria Neme Rosa Maria Neme Renée Zon Filippi Renée Zon Filippi Thiago Pinheiro Arrais Aloia Elisa Rampazo Prado Juliana Meola Carla de Azevedo Piccinato Carla de Azevedo Piccinato Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis Frontiers in Molecular Biosciences aromatase CYP19A1 STS HSD17B1 estradiol stromal cells |
| title | Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis |
| title_full | Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis |
| title_fullStr | Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis |
| title_full_unstemmed | Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis |
| title_short | Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis |
| title_sort | site specific regulation of sulfatase and aromatase pathways for estrogen production in endometriosis |
| topic | aromatase CYP19A1 STS HSD17B1 estradiol stromal cells |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.854991/full |
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