Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1
Exopolysaccharides produced by edible microorganisms exhibit excellent constructive physicochemical and significant biological activity, which provide advantages for the food or pharmaceutical industries. Two novel exopolysaccharides produced by <i>Debaryomyces hansenii</i> DH-1 were cha...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yajing Yang Guoqiang Chen Xiaoqi Zhao Xiaohe Cao Lei Wang Jingjiu Mu Fenghui Qi Lijuan Liu Haibo Zhang |
| author_facet | Yajing Yang Guoqiang Chen Xiaoqi Zhao Xiaohe Cao Lei Wang Jingjiu Mu Fenghui Qi Lijuan Liu Haibo Zhang |
| author_sort | Yajing Yang |
| collection | DOAJ |
| description | Exopolysaccharides produced by edible microorganisms exhibit excellent constructive physicochemical and significant biological activity, which provide advantages for the food or pharmaceutical industries. Two novel exopolysaccharides produced by <i>Debaryomyces hansenii</i> DH-1 were characterized, named S1 and S2, respectively. S1, with a molecular weight of 34.594 kDa, primarily consisted of mannose and glucose in a molar ratio of 12.19:1.00, which contained a backbone fragment of α-D-Manp-(1→4)-α-D-Manp-(1→2)-α-D-Glcp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-β-D-Manp-(1→. S2, with a molecular weight of 24.657 kDa, was mainly composed of mannose and galactose in a molar ratio of 4.00:1.00, which had a backbone fragment of α-D-Manp-(1→6)-β-D-Manp-(1→2)-α-D-Manp-(1→4)-α-D-Galp-(1→3)-β-D-Manp-(1→6)-α-D-Manp-(1→. Both S1 and S2 exhibited good thermal stability and potent hydroxyl radical scavenging activity, with ~98%. Moreover, S1 possessed an additional strong iron-reducing capacity. In vitro antitumor assays showed that S1 and S2 significantly inhibited the proliferation of Hela, HepG2, and PC-9 cancer cells. Moreover, PC-9 was more sensitive to S1 compared with S2. The above results indicate that S1 and S2 have great potential to be utilized as natural antioxidants and candidates for cancer treatment in the food and pharmaceutical industries. |
| first_indexed | 2024-03-11T10:00:22Z |
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| language | English |
| last_indexed | 2024-03-11T10:00:22Z |
| publishDate | 2022-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eb1a5468cf0743a281df0cabcef0bc292023-11-16T15:31:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124133510.3390/ijms24010335Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1Yajing Yang0Guoqiang Chen1Xiaoqi Zhao2Xiaohe Cao3Lei Wang4Jingjiu Mu5Fenghui Qi6Lijuan Liu7Haibo Zhang8College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaCAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaExopolysaccharides produced by edible microorganisms exhibit excellent constructive physicochemical and significant biological activity, which provide advantages for the food or pharmaceutical industries. Two novel exopolysaccharides produced by <i>Debaryomyces hansenii</i> DH-1 were characterized, named S1 and S2, respectively. S1, with a molecular weight of 34.594 kDa, primarily consisted of mannose and glucose in a molar ratio of 12.19:1.00, which contained a backbone fragment of α-D-Manp-(1→4)-α-D-Manp-(1→2)-α-D-Glcp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-β-D-Manp-(1→. S2, with a molecular weight of 24.657 kDa, was mainly composed of mannose and galactose in a molar ratio of 4.00:1.00, which had a backbone fragment of α-D-Manp-(1→6)-β-D-Manp-(1→2)-α-D-Manp-(1→4)-α-D-Galp-(1→3)-β-D-Manp-(1→6)-α-D-Manp-(1→. Both S1 and S2 exhibited good thermal stability and potent hydroxyl radical scavenging activity, with ~98%. Moreover, S1 possessed an additional strong iron-reducing capacity. In vitro antitumor assays showed that S1 and S2 significantly inhibited the proliferation of Hela, HepG2, and PC-9 cancer cells. Moreover, PC-9 was more sensitive to S1 compared with S2. The above results indicate that S1 and S2 have great potential to be utilized as natural antioxidants and candidates for cancer treatment in the food and pharmaceutical industries.https://www.mdpi.com/1422-0067/24/1/335<i>Debaryomyces hansenii</i>exopolysaccharidesstructure analysisantioxidant activityantitumor activitythermal property |
| spellingShingle | Yajing Yang Guoqiang Chen Xiaoqi Zhao Xiaohe Cao Lei Wang Jingjiu Mu Fenghui Qi Lijuan Liu Haibo Zhang Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 International Journal of Molecular Sciences <i>Debaryomyces hansenii</i> exopolysaccharides structure analysis antioxidant activity antitumor activity thermal property |
| title | Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 |
| title_full | Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 |
| title_fullStr | Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 |
| title_full_unstemmed | Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 |
| title_short | Structural Characterization, Antioxidant and Antitumor Activities of the Two Novel Exopolysaccharides Produced by <i>Debaryomyces hansenii</i> DH-1 |
| title_sort | structural characterization antioxidant and antitumor activities of the two novel exopolysaccharides produced by i debaryomyces hansenii i dh 1 |
| topic | <i>Debaryomyces hansenii</i> exopolysaccharides structure analysis antioxidant activity antitumor activity thermal property |
| url | https://www.mdpi.com/1422-0067/24/1/335 |
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