Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein
This study modified glass ionomer cement (GIC) by adding mimicked biological molecules to reduce cell death. GIC was modified to BIOGIC by adding chitosan and bovine serum albumin for enhancing protein release. The BIOGIC was supplemented with tricalcium phosphate (TCP) and recombinant translational...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/16/3341 |
| _version_ | 1797408273892114432 |
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| author | Prawichaya Sangsuwan Wilaiwan Chotigeat Sissada Tannukit Ureporn Kedjarune-Leggat |
| author_facet | Prawichaya Sangsuwan Wilaiwan Chotigeat Sissada Tannukit Ureporn Kedjarune-Leggat |
| author_sort | Prawichaya Sangsuwan |
| collection | DOAJ |
| description | This study modified glass ionomer cement (GIC) by adding mimicked biological molecules to reduce cell death. GIC was modified to BIOGIC by adding chitosan and bovine serum albumin for enhancing protein release. The BIOGIC was supplemented with tricalcium phosphate (TCP) and recombinant translationally controlled tumor protein (TCTP) to improve its biological properties. Four groups of materials, GIC, BIOGIC, BIOGIC+TCP, and BIOGIC + TCP + TCTP, were examined by XRD and SEM-EDX. TCTP released from the specimens was determined by an ELISA method. Human dental pulp stem cells (hDPSCs) were harvested and analyzed by MTT assay, apoptosis, gene expression, and cell differentiation. All groups had the same crystallization characteristic peaks of La<sub>2</sub>O<sub>3</sub>. The elemental compositions composed of La, Si, and Al are the main inorganic components. The results show that BIOGIC + TCP + TCTP presented significantly higher percentages of cell viability than other groups on day 1 to day 23 (<i>p</i> < 0.05), but were not different after day 24 to day 41 and had reduced cell apoptosis including BAX, TPT1, BCL-2, and Caspase-3. The BIOGIC + TCP + TCTP demonstrated higher odontoblast mineralization and differentiation markers including ALP activity, DSPP, DMP-1, ALP, BMP-2, and OPN. It enhanced cell proliferation and differentiation as well as mineralization with down-regulation of genes related to apoptosis compared with other groups. |
| first_indexed | 2024-03-09T03:57:04Z |
| format | Article |
| id | doaj.art-eb1a7ef86bbf412d9ab5d4c347584b5f |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:57:04Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-eb1a7ef86bbf412d9ab5d4c347584b5f2023-12-03T14:20:15ZengMDPI AGPolymers2073-43602022-08-011416334110.3390/polym14163341Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled ProteinPrawichaya Sangsuwan0Wilaiwan Chotigeat1Sissada Tannukit2Ureporn Kedjarune-Leggat3Molecular Biology and Bioinformatics Program, Biological Science Division, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandMolecular Biology and Bioinformatics Program, Biological Science Division, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandThis study modified glass ionomer cement (GIC) by adding mimicked biological molecules to reduce cell death. GIC was modified to BIOGIC by adding chitosan and bovine serum albumin for enhancing protein release. The BIOGIC was supplemented with tricalcium phosphate (TCP) and recombinant translationally controlled tumor protein (TCTP) to improve its biological properties. Four groups of materials, GIC, BIOGIC, BIOGIC+TCP, and BIOGIC + TCP + TCTP, were examined by XRD and SEM-EDX. TCTP released from the specimens was determined by an ELISA method. Human dental pulp stem cells (hDPSCs) were harvested and analyzed by MTT assay, apoptosis, gene expression, and cell differentiation. All groups had the same crystallization characteristic peaks of La<sub>2</sub>O<sub>3</sub>. The elemental compositions composed of La, Si, and Al are the main inorganic components. The results show that BIOGIC + TCP + TCTP presented significantly higher percentages of cell viability than other groups on day 1 to day 23 (<i>p</i> < 0.05), but were not different after day 24 to day 41 and had reduced cell apoptosis including BAX, TPT1, BCL-2, and Caspase-3. The BIOGIC + TCP + TCTP demonstrated higher odontoblast mineralization and differentiation markers including ALP activity, DSPP, DMP-1, ALP, BMP-2, and OPN. It enhanced cell proliferation and differentiation as well as mineralization with down-regulation of genes related to apoptosis compared with other groups.https://www.mdpi.com/2073-4360/14/16/3341translationally controlled proteinhuman dental pulp stem cellsglass ionomer cementtricalcium phosphateregenerative |
| spellingShingle | Prawichaya Sangsuwan Wilaiwan Chotigeat Sissada Tannukit Ureporn Kedjarune-Leggat Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein Polymers translationally controlled protein human dental pulp stem cells glass ionomer cement tricalcium phosphate regenerative |
| title | Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein |
| title_full | Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein |
| title_fullStr | Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein |
| title_full_unstemmed | Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein |
| title_short | Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein |
| title_sort | long term effect of modified glass ionomer cement with mimicked biological property of recombinant translationally controlled protein |
| topic | translationally controlled protein human dental pulp stem cells glass ionomer cement tricalcium phosphate regenerative |
| url | https://www.mdpi.com/2073-4360/14/16/3341 |
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