Collection and Storage of Human Plasma for Measurement of Oxylipins
Oxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/11/3/137 |
| _version_ | 1797395199205310464 |
|---|---|
| author | Kristen J. Polinski Michael Armstrong Jonathan Manke Jennifer Seifert Tessa Crume Fan Yang Michael Clare-Salzler V. Michael Holers Nichole Reisdorph Jill M. Norris |
| author_facet | Kristen J. Polinski Michael Armstrong Jonathan Manke Jennifer Seifert Tessa Crume Fan Yang Michael Clare-Salzler V. Michael Holers Nichole Reisdorph Jill M. Norris |
| author_sort | Kristen J. Polinski |
| collection | DOAJ |
| description | Oxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting samples, and if molecules are stable during collection and storage. We thereby built upon existing studies by examining the stability of an expanded panel of 90 oxylipins, including specialized pro-resolving lipid mediators (SPMs), in human plasma (<i>n</i> = 5 subjects) during sample collection, processing, and storage at −80 °C. Oxylipins were quantified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Blood samples collected in ethylenediaminetetraacetic acid (EDTA) or heparin followed by up to 2 h at room temperature prior to processing showed no significant differences in oxylipin concentrations compared to immediately processed samples, including the SPMs lipoxin A4 and resolvin D1. The majority of molecules, including SPMs, remained stable following storage for up to 1 year. However, in support of previous findings, changes were seen in a small subset of oxylipins including 12-HETE, TXB<sub>2</sub>, 14-HDHA, and 18-HEPE. Overall, this study showed that accurate measurements of most oxylipins can be obtained from stored EDTA or heparin plasma samples using LC/MS/MS. |
| first_indexed | 2024-03-09T00:31:01Z |
| format | Article |
| id | doaj.art-eb1e3a6a19624d14a7a666fa94790ba8 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T00:31:01Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-eb1e3a6a19624d14a7a666fa94790ba82023-12-11T18:32:16ZengMDPI AGMetabolites2218-19892021-02-0111313710.3390/metabo11030137Collection and Storage of Human Plasma for Measurement of OxylipinsKristen J. Polinski0Michael Armstrong1Jonathan Manke2Jennifer Seifert3Tessa Crume4Fan Yang5Michael Clare-Salzler6V. Michael Holers7Nichole Reisdorph8Jill M. Norris9Department of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADivision of Rheumatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610, USADivision of Rheumatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USAOxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting samples, and if molecules are stable during collection and storage. We thereby built upon existing studies by examining the stability of an expanded panel of 90 oxylipins, including specialized pro-resolving lipid mediators (SPMs), in human plasma (<i>n</i> = 5 subjects) during sample collection, processing, and storage at −80 °C. Oxylipins were quantified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Blood samples collected in ethylenediaminetetraacetic acid (EDTA) or heparin followed by up to 2 h at room temperature prior to processing showed no significant differences in oxylipin concentrations compared to immediately processed samples, including the SPMs lipoxin A4 and resolvin D1. The majority of molecules, including SPMs, remained stable following storage for up to 1 year. However, in support of previous findings, changes were seen in a small subset of oxylipins including 12-HETE, TXB<sub>2</sub>, 14-HDHA, and 18-HEPE. Overall, this study showed that accurate measurements of most oxylipins can be obtained from stored EDTA or heparin plasma samples using LC/MS/MS.https://www.mdpi.com/2218-1989/11/3/137lipid mediatorsoxylipinsstorageblood processingplasma |
| spellingShingle | Kristen J. Polinski Michael Armstrong Jonathan Manke Jennifer Seifert Tessa Crume Fan Yang Michael Clare-Salzler V. Michael Holers Nichole Reisdorph Jill M. Norris Collection and Storage of Human Plasma for Measurement of Oxylipins Metabolites lipid mediators oxylipins storage blood processing plasma |
| title | Collection and Storage of Human Plasma for Measurement of Oxylipins |
| title_full | Collection and Storage of Human Plasma for Measurement of Oxylipins |
| title_fullStr | Collection and Storage of Human Plasma for Measurement of Oxylipins |
| title_full_unstemmed | Collection and Storage of Human Plasma for Measurement of Oxylipins |
| title_short | Collection and Storage of Human Plasma for Measurement of Oxylipins |
| title_sort | collection and storage of human plasma for measurement of oxylipins |
| topic | lipid mediators oxylipins storage blood processing plasma |
| url | https://www.mdpi.com/2218-1989/11/3/137 |
| work_keys_str_mv | AT kristenjpolinski collectionandstorageofhumanplasmaformeasurementofoxylipins AT michaelarmstrong collectionandstorageofhumanplasmaformeasurementofoxylipins AT jonathanmanke collectionandstorageofhumanplasmaformeasurementofoxylipins AT jenniferseifert collectionandstorageofhumanplasmaformeasurementofoxylipins AT tessacrume collectionandstorageofhumanplasmaformeasurementofoxylipins AT fanyang collectionandstorageofhumanplasmaformeasurementofoxylipins AT michaelclaresalzler collectionandstorageofhumanplasmaformeasurementofoxylipins AT vmichaelholers collectionandstorageofhumanplasmaformeasurementofoxylipins AT nicholereisdorph collectionandstorageofhumanplasmaformeasurementofoxylipins AT jillmnorris collectionandstorageofhumanplasmaformeasurementofoxylipins |