How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings
Healthcare settings have been utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) since 2010. MALDI-TOF MS has various benefits over the conventional method of biochemical identification, including ease of use, speed, accuracy, and low cost. This app...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/11/1881 |
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| author | Ayman Elbehiry Musaad Aldubaib Adil Abalkhail Eman Marzouk Ahmad ALbeloushi Ihab Moussa Mai Ibrahem Hamad Albazie Abdullah Alqarni Sulaiman Anagreyyah Saleh Alghamdi Mohammed Rawway |
| author_facet | Ayman Elbehiry Musaad Aldubaib Adil Abalkhail Eman Marzouk Ahmad ALbeloushi Ihab Moussa Mai Ibrahem Hamad Albazie Abdullah Alqarni Sulaiman Anagreyyah Saleh Alghamdi Mohammed Rawway |
| author_sort | Ayman Elbehiry |
| collection | DOAJ |
| description | Healthcare settings have been utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) since 2010. MALDI-TOF MS has various benefits over the conventional method of biochemical identification, including ease of use, speed, accuracy, and low cost. This approach can solve many of the obstacles to identifying bacteria, fungi and viruses. As technology advanced, more and more databases kept track of spectra, allowing species with similar morphological, genotypic, and biochemical traits to be identified. Using MALDI-TOF MS for identification has become more accurate and quicker due to advances in sample preparation and database enrichment. Rapid sample detection and colony identification using MALDI-TOF MS have produced promising results. A key application of MALDI-TOF MS is quickly identifying highly virulent and drug-resistant diseases. Here, we present a review of the scientific literature assessing the effectiveness of MALDI-TOF MS for locating clinically relevant pathogenic bacteria, fungi, and viruses. MALDI-TOF MS is a useful strategy for locating clinical pathogens, however, it also has some drawbacks. A small number of spectra in the database and inherent similarities among organisms can make it difficult to distinguish between different species, which can result in misidentifications. The majority of the time additional testing may correct these problems, which happen very seldom. In conclusion, infectious illness diagnosis and clinical care are being revolutionized by the use of MALDI-TOF MS in the clinical microbiology laboratory. |
| first_indexed | 2024-03-09T18:34:47Z |
| format | Article |
| id | doaj.art-eb1efd0a79b94f0fbb94ba078a900753 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T18:34:47Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-eb1efd0a79b94f0fbb94ba078a9007532023-11-24T07:14:10ZengMDPI AGVaccines2076-393X2022-11-011011188110.3390/vaccines10111881How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare SettingsAyman Elbehiry0Musaad Aldubaib1Adil Abalkhail2Eman Marzouk3Ahmad ALbeloushi4Ihab Moussa5Mai Ibrahem6Hamad Albazie7Abdullah Alqarni8Sulaiman Anagreyyah9Saleh Alghamdi10Mohammed Rawway11Department of Public Health, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah 52741, Saudi ArabiaDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 52571, Saudi ArabiaDepartment of Public Health, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah 52741, Saudi ArabiaDepartment of Public Health, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah 52741, Saudi ArabiaAl Bukayriyah General Hospital, Qassim, Al Bukayriyah 52725, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Public Health, College of Applied Medical Science, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Public Health, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah 52741, Saudi ArabiaDepartment of Support Service, King Fahad Armed Hospital, Jeddah 23311, Saudi ArabiaDepartment of Preventive Medicine, King Fahad Armed Hospital, Jeddah 23311, Saudi ArabiaDepartment of Biomedical Engineering, King Fahad Armed Hospital, Jeddah 23311, Saudi ArabiaBiology Department, College of Science, Jouf University, Sakaka 42421, Saudi ArabiaHealthcare settings have been utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) since 2010. MALDI-TOF MS has various benefits over the conventional method of biochemical identification, including ease of use, speed, accuracy, and low cost. This approach can solve many of the obstacles to identifying bacteria, fungi and viruses. As technology advanced, more and more databases kept track of spectra, allowing species with similar morphological, genotypic, and biochemical traits to be identified. Using MALDI-TOF MS for identification has become more accurate and quicker due to advances in sample preparation and database enrichment. Rapid sample detection and colony identification using MALDI-TOF MS have produced promising results. A key application of MALDI-TOF MS is quickly identifying highly virulent and drug-resistant diseases. Here, we present a review of the scientific literature assessing the effectiveness of MALDI-TOF MS for locating clinically relevant pathogenic bacteria, fungi, and viruses. MALDI-TOF MS is a useful strategy for locating clinical pathogens, however, it also has some drawbacks. A small number of spectra in the database and inherent similarities among organisms can make it difficult to distinguish between different species, which can result in misidentifications. The majority of the time additional testing may correct these problems, which happen very seldom. In conclusion, infectious illness diagnosis and clinical care are being revolutionized by the use of MALDI-TOF MS in the clinical microbiology laboratory.https://www.mdpi.com/2076-393X/10/11/1881MALDI-TOF MShealthcare settingsmicrobial identificationantimicrobial resistance |
| spellingShingle | Ayman Elbehiry Musaad Aldubaib Adil Abalkhail Eman Marzouk Ahmad ALbeloushi Ihab Moussa Mai Ibrahem Hamad Albazie Abdullah Alqarni Sulaiman Anagreyyah Saleh Alghamdi Mohammed Rawway How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings Vaccines MALDI-TOF MS healthcare settings microbial identification antimicrobial resistance |
| title | How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings |
| title_full | How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings |
| title_fullStr | How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings |
| title_full_unstemmed | How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings |
| title_short | How MALDI-TOF Mass Spectrometry Technology Contributes to Microbial Infection Control in Healthcare Settings |
| title_sort | how maldi tof mass spectrometry technology contributes to microbial infection control in healthcare settings |
| topic | MALDI-TOF MS healthcare settings microbial identification antimicrobial resistance |
| url | https://www.mdpi.com/2076-393X/10/11/1881 |
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