Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice
Lactobacilli (n = 24), isolated from human infants and yogurt, showed variable in vitro activity against Salmonella enteritidis (8.0 ± 1.0 to 16.6 ± 0.5 mm) and other gut pathogens (9.0 ± 1.0 to 15.3 ± 0.5 mm), as determined by a well diffusion assay. The isolates were identified as Limosilactobacil...
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-08-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-0100 |
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| author | Mustafa Amina Nawaz Muhammad Rabbani Masood Tayyab Muhammad Khan Madiha |
| author_facet | Mustafa Amina Nawaz Muhammad Rabbani Masood Tayyab Muhammad Khan Madiha |
| author_sort | Mustafa Amina |
| collection | DOAJ |
| description | Lactobacilli (n = 24), isolated from human infants and yogurt, showed variable in vitro activity against Salmonella enteritidis (8.0 ± 1.0 to 16.6 ± 0.5 mm) and other gut pathogens (9.0 ± 1.0 to 15.3 ± 0.5 mm), as determined by a well diffusion assay. The isolates were identified as Limosilactobacillus fermentum (FY1, FY3, FY4, IL2, and IL5), Lactobacillus delbrueckii (FY6 and FY7), Lactobacillus sp. (IL7), and Lactobacillus gasseri (IL12). All isolates showed variable in vitro tolerance to acidic pH for 3 h and visible growth at pH 4 and in the presence of 0.3% ox-bile. The antibiotic susceptibility profile of Lactobacillus isolates indicated resistance against vancomycin, ciprofloxacin, streptomycin, and lincomycin. Isolates had variable auto-aggregation and showed variable capabilities to co-aggregate with S. enteritidis. Based on all tested parameters, L. fermentum IL2, L. fermentum IL5, and L. gasseri IL12 were selected for co-culture experiments, followed by in vivo evaluation in Balb/c mice. All the selected isolates resulted in a 100% reduction in S. enteritidis in broth. Lactobacillus isolates efficiently colonized mouse guts and inhibited S. enteritidis colonization. Overall, there was ≥99.06% and ≤4.32 Mean log10 reduction in Salmonella counts in mice feces within 7 days. The study, thus, provided characterized lactobacilli that could be considered as potential ingredients for probiotic formulations intended to prevent S. enteritidis infection in humans. |
| first_indexed | 2024-12-10T04:49:27Z |
| format | Article |
| id | doaj.art-eb24bdc430c34772a61b20481059c780 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-10T04:49:27Z |
| publishDate | 2022-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-eb24bdc430c34772a61b20481059c7802022-12-22T02:01:39ZengDe GruyterOpen Life Sciences2391-54122022-08-0117197899010.1515/biol-2022-0100Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in miceMustafa Amina0Nawaz Muhammad1Rabbani Masood2Tayyab Muhammad3Khan Madiha4Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, PakistanInstitute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, PakistanDepartment of Microbiology, University of Central Punjab, Lahore, 54000, Punjab, PakistanLactobacilli (n = 24), isolated from human infants and yogurt, showed variable in vitro activity against Salmonella enteritidis (8.0 ± 1.0 to 16.6 ± 0.5 mm) and other gut pathogens (9.0 ± 1.0 to 15.3 ± 0.5 mm), as determined by a well diffusion assay. The isolates were identified as Limosilactobacillus fermentum (FY1, FY3, FY4, IL2, and IL5), Lactobacillus delbrueckii (FY6 and FY7), Lactobacillus sp. (IL7), and Lactobacillus gasseri (IL12). All isolates showed variable in vitro tolerance to acidic pH for 3 h and visible growth at pH 4 and in the presence of 0.3% ox-bile. The antibiotic susceptibility profile of Lactobacillus isolates indicated resistance against vancomycin, ciprofloxacin, streptomycin, and lincomycin. Isolates had variable auto-aggregation and showed variable capabilities to co-aggregate with S. enteritidis. Based on all tested parameters, L. fermentum IL2, L. fermentum IL5, and L. gasseri IL12 were selected for co-culture experiments, followed by in vivo evaluation in Balb/c mice. All the selected isolates resulted in a 100% reduction in S. enteritidis in broth. Lactobacillus isolates efficiently colonized mouse guts and inhibited S. enteritidis colonization. Overall, there was ≥99.06% and ≤4.32 Mean log10 reduction in Salmonella counts in mice feces within 7 days. The study, thus, provided characterized lactobacilli that could be considered as potential ingredients for probiotic formulations intended to prevent S. enteritidis infection in humans.https://doi.org/10.1515/biol-2022-0100lactobacillisalmonella enteritidismiceyogurthuman infants |
| spellingShingle | Mustafa Amina Nawaz Muhammad Rabbani Masood Tayyab Muhammad Khan Madiha Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice Open Life Sciences lactobacilli salmonella enteritidis mice yogurt human infants |
| title | Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| title_full | Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| title_fullStr | Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| title_full_unstemmed | Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| title_short | Characterization and evaluation of anti-Salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| title_sort | characterization and evaluation of anti salmonella enteritidis activity of indigenous probiotic lactobacilli in mice |
| topic | lactobacilli salmonella enteritidis mice yogurt human infants |
| url | https://doi.org/10.1515/biol-2022-0100 |
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