The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing
Erwinia amylovora is a gram-negative phytopathogen that causes fire blight of pome fruit and related members of the family Rosaceae. We sequenced the putative autoinducer-2 (AI-2) synthase gene luxS from E. amylovora. Diversity analysis indicated that this gene is extremely conserved among E. amylov...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1284 |
| _version_ | 1811278085740298240 |
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| author | Fabio Rezzonico Brion Duffy |
| author_facet | Fabio Rezzonico Brion Duffy |
| author_sort | Fabio Rezzonico |
| collection | DOAJ |
| description | Erwinia amylovora is a gram-negative phytopathogen that causes fire blight of pome fruit and related members of the family Rosaceae. We sequenced the putative autoinducer-2 (AI-2) synthase gene luxS from E. amylovora. Diversity analysis indicated that this gene is extremely conserved among E. amylovora strains. Quorum sensing mediated by LuxS has been implicated in coordinated gene expression, growth, and virulence in other enterobacteria; however, our evidence suggests this is not the function in E. amylovora. Mutational analysis pointed to a role in colonization of apple blossoms, the primary infection court for fire blight, although little if any role in virulence on apple shoots and pear fruit was observed. Expression of key virulence genes hrpL and dspA/E was reduced in mutants of two E. amylovora strains. Stronger effects on gene expression were observed for metabolic genes involved in the activated methyl cycle with mutants having greater levels of expression. No quorum-sensing effect was observed in co-culture experiments with wild-type and mutant strains either in vitro or in apple blossoms. Known receptors essential for AI-2 quorum sensing, the LuxPQ sensor kinase or the Lsr ABC-transporter, are absent in E. amylovora, further suggesting a primarily metabolic role for luxS in this bacterium. |
| first_indexed | 2024-04-13T00:29:31Z |
| format | Article |
| id | doaj.art-eb26a47848db4e95b340d7c1eab083ed |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:29:31Z |
| publishDate | 2007-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-eb26a47848db4e95b340d7c1eab083ed2022-12-22T03:10:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-10-0120101284129710.1094/MPMI-20-10-1284The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum SensingFabio RezzonicoBrion DuffyErwinia amylovora is a gram-negative phytopathogen that causes fire blight of pome fruit and related members of the family Rosaceae. We sequenced the putative autoinducer-2 (AI-2) synthase gene luxS from E. amylovora. Diversity analysis indicated that this gene is extremely conserved among E. amylovora strains. Quorum sensing mediated by LuxS has been implicated in coordinated gene expression, growth, and virulence in other enterobacteria; however, our evidence suggests this is not the function in E. amylovora. Mutational analysis pointed to a role in colonization of apple blossoms, the primary infection court for fire blight, although little if any role in virulence on apple shoots and pear fruit was observed. Expression of key virulence genes hrpL and dspA/E was reduced in mutants of two E. amylovora strains. Stronger effects on gene expression were observed for metabolic genes involved in the activated methyl cycle with mutants having greater levels of expression. No quorum-sensing effect was observed in co-culture experiments with wild-type and mutant strains either in vitro or in apple blossoms. Known receptors essential for AI-2 quorum sensing, the LuxPQ sensor kinase or the Lsr ABC-transporter, are absent in E. amylovora, further suggesting a primarily metabolic role for luxS in this bacterium.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1284sulfur metabolism |
| spellingShingle | Fabio Rezzonico Brion Duffy The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing Molecular Plant-Microbe Interactions sulfur metabolism |
| title | The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing |
| title_full | The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing |
| title_fullStr | The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing |
| title_full_unstemmed | The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing |
| title_short | The Role of luxS in the Fire Blight Pathogen Erwinia amylovora Is Limited to Metabolism and Does Not Involve Quorum Sensing |
| title_sort | role of luxs in the fire blight pathogen erwinia amylovora is limited to metabolism and does not involve quorum sensing |
| topic | sulfur metabolism |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1284 |
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