Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity
The banana weevil (<i>Cosmopolites sordidus</i>) and the burrowing nematode <i>Radopholus similis</i> represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic <i>Fusarium oxysporum</i> (isolate V5w2) and...
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MDPI AG
2022-01-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/2/125 |
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| author | James Kisaakye Hendrika Fourie Solveig Haukeland Joseph Kisitu Solomy Nakimera Laura Cortada Sevgan Subramanian Danny Coyne |
| author_facet | James Kisaakye Hendrika Fourie Solveig Haukeland Joseph Kisitu Solomy Nakimera Laura Cortada Sevgan Subramanian Danny Coyne |
| author_sort | James Kisaakye |
| collection | DOAJ |
| description | The banana weevil (<i>Cosmopolites sordidus</i>) and the burrowing nematode <i>Radopholus similis</i> represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic <i>Fusarium oxysporum</i> (isolate V5w2) and the entomopathogenic <i>Beauveria bassiana</i> (isolate WA) have been shown to increase host resistance to various banana pests and diseases. However, there is limited data on how the combined inoculation of these isolates would affect field performance of bananas. In this study, the fungal endophytes were inoculated separately and in combination. Tissue cultured plantlets of cooking banana cultivar Mbwazirume and dessert banana cultivar Grande Naine were inoculated by root drenching with a suspension of 1.0 × 10<sup>7</sup> spores mL<sup>−1</sup> of the endophytes on three occasions, separated 4 weeks apart, before transplanting into the field. Each plantlet was further inoculated with 1800 nematodes, composed primarily of <i>R. similis</i>. Inoculation of banana plants with the fungal endophytes significantly reduced nematode densities by >34%. Similarly, plant toppling was lower in the endophyte-enhanced plants (<16.5%) compared with the control (23.3%). We also observed improved yield of the first crop cycle in the endophyte-enhanced plants, which yielded >11 t ha<sup>−1</sup> year<sup>−1</sup> versus 9 t ha<sup>−1</sup> year<sup>−1</sup> achieved in the non-inoculated plants. These findings demonstrate the benefits of fungal endophytes in improving the yield of both cooking and dessert bananas via suppression of nematode densities and nematode-related damage. |
| first_indexed | 2024-03-09T22:54:43Z |
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| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T22:54:43Z |
| publishDate | 2022-01-01 |
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| series | Agriculture |
| spelling | doaj.art-eb26a926cdc142ceaae60ad64a7330a32023-11-23T18:15:00ZengMDPI AGAgriculture2077-04722022-01-0112212510.3390/agriculture12020125Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) ProductivityJames Kisaakye0Hendrika Fourie1Solveig Haukeland2Joseph Kisitu3Solomy Nakimera4Laura Cortada5Sevgan Subramanian6Danny Coyne7International Centre of Insect Physiology and Ecology (<i>icipe</i>), P.O. Box 30772, Nairobi 00100, KenyaUnit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South AfricaInternational Centre of Insect Physiology and Ecology (<i>icipe</i>), P.O. Box 30772, Nairobi 00100, KenyaInternational Institute of Tropical Agriculture (IITA), P.O. Box 7878, Kampala 10102, UgandaInternational Institute of Tropical Agriculture (IITA), P.O. Box 7878, Kampala 10102, Uganda<i>icipe</i> Campus, International Institute of Tropical Agriculture (IITA), P.O. Box 30772, Nairobi 00100, KenyaInternational Centre of Insect Physiology and Ecology (<i>icipe</i>), P.O. Box 30772, Nairobi 00100, Kenya<i>icipe</i> Campus, International Institute of Tropical Agriculture (IITA), P.O. Box 30772, Nairobi 00100, KenyaThe banana weevil (<i>Cosmopolites sordidus</i>) and the burrowing nematode <i>Radopholus similis</i> represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic <i>Fusarium oxysporum</i> (isolate V5w2) and the entomopathogenic <i>Beauveria bassiana</i> (isolate WA) have been shown to increase host resistance to various banana pests and diseases. However, there is limited data on how the combined inoculation of these isolates would affect field performance of bananas. In this study, the fungal endophytes were inoculated separately and in combination. Tissue cultured plantlets of cooking banana cultivar Mbwazirume and dessert banana cultivar Grande Naine were inoculated by root drenching with a suspension of 1.0 × 10<sup>7</sup> spores mL<sup>−1</sup> of the endophytes on three occasions, separated 4 weeks apart, before transplanting into the field. Each plantlet was further inoculated with 1800 nematodes, composed primarily of <i>R. similis</i>. Inoculation of banana plants with the fungal endophytes significantly reduced nematode densities by >34%. Similarly, plant toppling was lower in the endophyte-enhanced plants (<16.5%) compared with the control (23.3%). We also observed improved yield of the first crop cycle in the endophyte-enhanced plants, which yielded >11 t ha<sup>−1</sup> year<sup>−1</sup> versus 9 t ha<sup>−1</sup> year<sup>−1</sup> achieved in the non-inoculated plants. These findings demonstrate the benefits of fungal endophytes in improving the yield of both cooking and dessert bananas via suppression of nematode densities and nematode-related damage.https://www.mdpi.com/2077-0472/12/2/125banana nematodesbiological controlendophytesmicrobial antagonistsbio-enhanced plants<i>Cosmopolites sordidus</i> |
| spellingShingle | James Kisaakye Hendrika Fourie Solveig Haukeland Joseph Kisitu Solomy Nakimera Laura Cortada Sevgan Subramanian Danny Coyne Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity Agriculture banana nematodes biological control endophytes microbial antagonists bio-enhanced plants <i>Cosmopolites sordidus</i> |
| title | Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity |
| title_full | Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity |
| title_fullStr | Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity |
| title_full_unstemmed | Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity |
| title_short | Endophytic Non-Pathogenic <i>Fusarium oxysporum</i>-Derived Dual Benefit for Nematode Management and Improved Banana (<i>Musa</i> spp.) Productivity |
| title_sort | endophytic non pathogenic i fusarium oxysporum i derived dual benefit for nematode management and improved banana i musa i spp productivity |
| topic | banana nematodes biological control endophytes microbial antagonists bio-enhanced plants <i>Cosmopolites sordidus</i> |
| url | https://www.mdpi.com/2077-0472/12/2/125 |
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