Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae
Abstract In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehen...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03095-2 |
| _version_ | 1797398176861257728 |
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| author | Qian Li Peng Feng Hao Tang Fujia Lu Borui Mou Lan Zhao Nan Li Yaojun Yang Chun Fu Wencong Long Ximeng Xiao Chaohao Li Wei Wu Gang Wang Beidong Liu Tianle Tang Menggen Ma Hanyu Wang |
| author_facet | Qian Li Peng Feng Hao Tang Fujia Lu Borui Mou Lan Zhao Nan Li Yaojun Yang Chun Fu Wencong Long Ximeng Xiao Chaohao Li Wei Wu Gang Wang Beidong Liu Tianle Tang Menggen Ma Hanyu Wang |
| author_sort | Qian Li |
| collection | DOAJ |
| description | Abstract In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehensive analysis was conducted using genome-wide deletion library scanning and SGAtools, resulting in the identification of 294 genes associated with HMF tolerance in S. cerevisiae. Further KEGG and GO enrichment analysis revealed the involvement of genes OCA1 and SIW14 in the protein phosphorylation pathway, underscoring their role in HMF tolerance. Spot test validation and subcellular structure observation demonstrated that, following a 3-h treatment with 60 mM HMF, the SIW14 gene knockout strain exhibited a 12.68% increase in cells with abnormal endoplasmic reticulum (ER) and a 22.41% increase in the accumulation of reactive oxygen species compared to the BY4741 strain. These findings indicate that the SIW14 gene contributes to the protection of the ER structure within the cell and facilitates the clearance of reactive oxygen species, thereby confirming its significance as a key gene for HMF tolerance in S. cerevisiae. |
| first_indexed | 2024-03-09T01:20:58Z |
| format | Article |
| id | doaj.art-eb2afb3a576e45eb8d3cad23f2fb3bd4 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T01:20:58Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-eb2afb3a576e45eb8d3cad23f2fb3bd42023-12-10T12:09:34ZengBMCBMC Microbiology1471-21802023-12-0123111210.1186/s12866-023-03095-2Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiaeQian Li0Peng Feng1Hao Tang2Fujia Lu3Borui Mou4Lan Zhao5Nan Li6Yaojun Yang7Chun Fu8Wencong Long9Ximeng Xiao10Chaohao Li11Wei Wu12Gang Wang13Beidong Liu14Tianle Tang15Menggen Ma16Hanyu Wang17College of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityKey Laboratory of Land Resources Evaluation and Monitoring in Southwest, Ministry of Education, Sichuan Normal UniversityCollege of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityJiangxi Forestry Science and Technology Promotion and Publicity Education CenterCollege of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityCollege of Life Science, Leshan Normal UniversityLeshan Institute of Product Quality Supervision and TestingLeshan Institute of Product Quality Supervision and TestingCollege of Life Science, Leshan Normal UniversityDepartment of Chemistry and Molecular Biology, University of GothenburgKey Laboratory of Tropical Transitional Medicine of Ministry of Education, Hainan Medical UniversityDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural UniversityCollege of Life Science, Leshan Normal UniversityAbstract In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehensive analysis was conducted using genome-wide deletion library scanning and SGAtools, resulting in the identification of 294 genes associated with HMF tolerance in S. cerevisiae. Further KEGG and GO enrichment analysis revealed the involvement of genes OCA1 and SIW14 in the protein phosphorylation pathway, underscoring their role in HMF tolerance. Spot test validation and subcellular structure observation demonstrated that, following a 3-h treatment with 60 mM HMF, the SIW14 gene knockout strain exhibited a 12.68% increase in cells with abnormal endoplasmic reticulum (ER) and a 22.41% increase in the accumulation of reactive oxygen species compared to the BY4741 strain. These findings indicate that the SIW14 gene contributes to the protection of the ER structure within the cell and facilitates the clearance of reactive oxygen species, thereby confirming its significance as a key gene for HMF tolerance in S. cerevisiae.https://doi.org/10.1186/s12866-023-03095-2Saccharomyces cerevisiaeSIW145-HydroxymethylfurfuralTolerance |
| spellingShingle | Qian Li Peng Feng Hao Tang Fujia Lu Borui Mou Lan Zhao Nan Li Yaojun Yang Chun Fu Wencong Long Ximeng Xiao Chaohao Li Wei Wu Gang Wang Beidong Liu Tianle Tang Menggen Ma Hanyu Wang Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae BMC Microbiology Saccharomyces cerevisiae SIW14 5-Hydroxymethylfurfural Tolerance |
| title | Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae |
| title_full | Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae |
| title_fullStr | Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae |
| title_full_unstemmed | Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae |
| title_short | Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae |
| title_sort | genome wide identification of resistance genes and cellular analysis of key gene knockout strain under 5 hydroxymethylfurfural stress in saccharomyces cerevisiae |
| topic | Saccharomyces cerevisiae SIW14 5-Hydroxymethylfurfural Tolerance |
| url | https://doi.org/10.1186/s12866-023-03095-2 |
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