<i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats
<i>Aspergillus</i>-derived cellulase, which is involved in the production of <i>Aspergillus</i>-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary <i>Aspergillus</i> cellulase on health is poorly understood. P...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/2/71 |
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| author | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi |
| author_facet | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi |
| author_sort | Yongshou Yang |
| collection | DOAJ |
| description | <i>Aspergillus</i>-derived cellulase, which is involved in the production of <i>Aspergillus</i>-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary <i>Aspergillus</i> cellulase on health is poorly understood. Previously, we discovered that supplemental <i>Aspergillus</i>-derived protease and lipase preparations had substantial bifidogenic effects on rats fed a high-fat diet. Therefore, this study reports on the effects of a 0.1% dietary <i>Aspergillus</i>-derived cellulase preparation (CEL) on the gut microbiota of rats fed a high-fat diet. Gene sequencing analysis of 16S rRNA revealed that CEL treatment markedly affected the microbiota profiles of the cecal contents (<i>p</i> < 0.05). Notably, CEL markedly increased the relative abundance (RA) of typical probiotics, such as <i>Bifidobacterium</i> and <i>Lactobacillus</i>, at the genus level (28- and 5-fold, respectively, <i>p</i> < 0.05). Similarly, at the family level, CEL treatment significantly increased the RA of Bifidobacteriaceae and Lactobacillaceae (<i>p</i> < 0.05). Furthermore, CEL increased the RA of other genera, such as <i>Collinsella</i> and <i>Enterococcus</i>, but decreased the RA of <i>Oscillospira</i>, <i>Dorea</i> and <i>Coprobacillus</i> (<i>p</i> < 0.05). The effects on these genera are similar to those reported for typical prebiotic oligosaccharides. Overall, this study demonstrates the prebiotic-like effects of dietary CEL by significantly increasing <i>Bifidobacterium</i> and <i>Lactobacillus</i> abundance. |
| first_indexed | 2024-03-09T22:01:06Z |
| format | Article |
| id | doaj.art-eb325a010c2f4ce7b320addac9a88dd8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T22:01:06Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-eb325a010c2f4ce7b320addac9a88dd82023-11-23T19:50:12ZengMDPI AGFermentation2311-56372022-02-01827110.3390/fermentation8020071<i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in RatsYongshou Yang0Thanutchaporn Kumrungsee1Norihisa Kato2Shinji Fukuda3Manabu Kuroda4Shotaro Yamaguchi5School of Life Sciences, Anhui University, Hefei 23061, ChinaGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi, Hiroshima 739-8528, JapanGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi, Hiroshima 739-8528, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, JapanAmano Enzyme Inc., Nagoya 460-8630, JapanAmano Enzyme Inc., Nagoya 460-8630, Japan<i>Aspergillus</i>-derived cellulase, which is involved in the production of <i>Aspergillus</i>-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary <i>Aspergillus</i> cellulase on health is poorly understood. Previously, we discovered that supplemental <i>Aspergillus</i>-derived protease and lipase preparations had substantial bifidogenic effects on rats fed a high-fat diet. Therefore, this study reports on the effects of a 0.1% dietary <i>Aspergillus</i>-derived cellulase preparation (CEL) on the gut microbiota of rats fed a high-fat diet. Gene sequencing analysis of 16S rRNA revealed that CEL treatment markedly affected the microbiota profiles of the cecal contents (<i>p</i> < 0.05). Notably, CEL markedly increased the relative abundance (RA) of typical probiotics, such as <i>Bifidobacterium</i> and <i>Lactobacillus</i>, at the genus level (28- and 5-fold, respectively, <i>p</i> < 0.05). Similarly, at the family level, CEL treatment significantly increased the RA of Bifidobacteriaceae and Lactobacillaceae (<i>p</i> < 0.05). Furthermore, CEL increased the RA of other genera, such as <i>Collinsella</i> and <i>Enterococcus</i>, but decreased the RA of <i>Oscillospira</i>, <i>Dorea</i> and <i>Coprobacillus</i> (<i>p</i> < 0.05). The effects on these genera are similar to those reported for typical prebiotic oligosaccharides. Overall, this study demonstrates the prebiotic-like effects of dietary CEL by significantly increasing <i>Bifidobacterium</i> and <i>Lactobacillus</i> abundance.https://www.mdpi.com/2311-5637/8/2/71fermentation foods<i>Aspergillus</i>cellulase<i>Bifidobacterium</i><i>Lactobacillus</i>prebiotic |
| spellingShingle | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats Fermentation fermentation foods <i>Aspergillus</i> cellulase <i>Bifidobacterium</i> <i>Lactobacillus</i> prebiotic |
| title | <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats |
| title_full | <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats |
| title_fullStr | <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats |
| title_full_unstemmed | <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats |
| title_short | <i>Aspergillus-</i>Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats |
| title_sort | i aspergillus i derived cellulase preparation exhibits prebiotic like effects on gut microbiota in rats |
| topic | fermentation foods <i>Aspergillus</i> cellulase <i>Bifidobacterium</i> <i>Lactobacillus</i> prebiotic |
| url | https://www.mdpi.com/2311-5637/8/2/71 |
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