Characterization of multitype colonies originating from porcine blastocysts produced in vitro
Many types of embryonic stem cells have been induced from pre-implantation blastocysts to study the specification of early lineages. Various cell lines have been established using chemicals, including excessive inhibitory molecules. Previous studies have also aimed to purify cell populations represe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.918222/full |
| _version_ | 1818065578373414912 |
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| author | Jong-Nam Oh Jinsol Jeong Mingyun Lee Gyung Cheol Choe Dong-Kyung Lee Kwang-Hwan Choi Seung-Hun Kim Chang-Kyu Lee Chang-Kyu Lee |
| author_facet | Jong-Nam Oh Jinsol Jeong Mingyun Lee Gyung Cheol Choe Dong-Kyung Lee Kwang-Hwan Choi Seung-Hun Kim Chang-Kyu Lee Chang-Kyu Lee |
| author_sort | Jong-Nam Oh |
| collection | DOAJ |
| description | Many types of embryonic stem cells have been induced from pre-implantation blastocysts to study the specification of early lineages. Various cell lines have been established using chemicals, including excessive inhibitory molecules. Previous studies have also aimed to purify cell populations representing a single embryonic lineage from a protocol. In this study, we used a novel culture condition to induce cells from blastocyst seeding and analyzed their characteristics. Next, signaling inhibitors were introduced during the cell culture period. Furthermore, we investigated the cell types using RNA sequencing. Each type of cell population showed a distinct morphology and reactivity with alkaline phosphatase. Marker proteins enabled each cell type to be distinguished by immunocytochemistry, and genes such as Sox17, Gata4, Gata6, T, and Cdx2 showed applicability for the discrimination of cell types. Signaling inhibitors suppressed the production of some cell types, and gene expression and marker protein patterns were collapsed. RNA-sequencing suggested cell-type-specific marker genes and the correlation among samples. In conclusion, four types of cells could be induced from porcine embryos using a single protocol, and they could be isolated manually. Our data will help promote the study of lineage segregation based on embryonic cells. |
| first_indexed | 2024-12-10T14:54:07Z |
| format | Article |
| id | doaj.art-eb362ce520704510b204db6dbb6b329c |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-10T14:54:07Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-eb362ce520704510b204db6dbb6b329c2022-12-22T01:44:23ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.918222918222Characterization of multitype colonies originating from porcine blastocysts produced in vitroJong-Nam Oh0Jinsol Jeong1Mingyun Lee2Gyung Cheol Choe3Dong-Kyung Lee4Kwang-Hwan Choi5Seung-Hun Kim6Chang-Kyu Lee7Chang-Kyu Lee8Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDesigned Animal and Transplantation Research Institute (DATRI), Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, South KoreaMany types of embryonic stem cells have been induced from pre-implantation blastocysts to study the specification of early lineages. Various cell lines have been established using chemicals, including excessive inhibitory molecules. Previous studies have also aimed to purify cell populations representing a single embryonic lineage from a protocol. In this study, we used a novel culture condition to induce cells from blastocyst seeding and analyzed their characteristics. Next, signaling inhibitors were introduced during the cell culture period. Furthermore, we investigated the cell types using RNA sequencing. Each type of cell population showed a distinct morphology and reactivity with alkaline phosphatase. Marker proteins enabled each cell type to be distinguished by immunocytochemistry, and genes such as Sox17, Gata4, Gata6, T, and Cdx2 showed applicability for the discrimination of cell types. Signaling inhibitors suppressed the production of some cell types, and gene expression and marker protein patterns were collapsed. RNA-sequencing suggested cell-type-specific marker genes and the correlation among samples. In conclusion, four types of cells could be induced from porcine embryos using a single protocol, and they could be isolated manually. Our data will help promote the study of lineage segregation based on embryonic cells.https://www.frontiersin.org/articles/10.3389/fcell.2022.918222/fullembryolineagesegregationRNA-seqblastocyst attachment |
| spellingShingle | Jong-Nam Oh Jinsol Jeong Mingyun Lee Gyung Cheol Choe Dong-Kyung Lee Kwang-Hwan Choi Seung-Hun Kim Chang-Kyu Lee Chang-Kyu Lee Characterization of multitype colonies originating from porcine blastocysts produced in vitro Frontiers in Cell and Developmental Biology embryo lineage segregation RNA-seq blastocyst attachment |
| title | Characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| title_full | Characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| title_fullStr | Characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| title_full_unstemmed | Characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| title_short | Characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| title_sort | characterization of multitype colonies originating from porcine blastocysts produced in vitro |
| topic | embryo lineage segregation RNA-seq blastocyst attachment |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.918222/full |
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