Amperometric biosensor for ascorbic acid
A L-ascorbic acid biosensor based on ascorbate oxidase has been developed. The enzyme was extracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L -1 phosphate buffer, pH 5.8 containing 0.5 mol L -1 NaCl. After the dialysis versus phosphate buffer 0.05 mol L -1 pH 5.8, the enzym...
Main Authors: | Inês N. Tomita, Alexandra Manzoli, Fernando L. Fertonani, Hideko Yamanaka |
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Format: | Article |
Language: | English |
Published: |
Universidade Estadual Paulista
2018-05-01
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Series: | Eclética Química |
Online Access: | https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/459 |
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