Fecal neutral steroids and bile acids from germfree rats
The amount and composition of fecal neutral sterols and bile acids excreted by adult male germfree and conventional rats have been determined. The amounts of neutral sterols excreted were 12.8 (germfree) and 19.5 (conventional) mg/kg of body wt per day. The germfree rats excreted cholesterol and lat...
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| Format: | Article |
| Language: | English |
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Elsevier
1969-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520430408 |
| _version_ | 1818941234087985152 |
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| author | Thomas F. Kellogg Bernard S. Wostmann |
| author_facet | Thomas F. Kellogg Bernard S. Wostmann |
| author_sort | Thomas F. Kellogg |
| collection | DOAJ |
| description | The amount and composition of fecal neutral sterols and bile acids excreted by adult male germfree and conventional rats have been determined. The amounts of neutral sterols excreted were 12.8 (germfree) and 19.5 (conventional) mg/kg of body wt per day. The germfree rats excreted cholesterol and lathosterol (methostenol was not assayed); the conventional rats excreted coprostanol and coprostanone in addition. The amounts of bile acids excreted were 11.3 (germfree) and 21.4 (conventional) mg/kg of body wt per day. The bile acids excreted by the rats were tentatively identified as tauro-β-muricholate, tauro-α-muricholate, and tauro-cholate, besides an unidentified component. The conventional rats excreted the corresponding unconjugated acids as well as many other unconjugated bile acids.No significant correlation was found between the amount of coprosterols and the total amount of neutral sterols excreted by the conventional rats. This suggests that bacterial reduction of cholesterol is not an important mechanism of increasing neutral sterol excretion of conventional rats as compared to germfree rats. Evidence is presented that suggests that this difference in neutral sterol excretion is due to changes in intestinal secretion and sloughing between the two types of animal. The factors reponsible for the differences in bile acid excretion have not been identified. |
| first_indexed | 2024-12-20T06:52:17Z |
| format | Article |
| id | doaj.art-eb3dd0b3016a40b58a61889a10e8b357 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T06:52:17Z |
| publishDate | 1969-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-eb3dd0b3016a40b58a61889a10e8b3572022-12-21T19:49:31ZengElsevierJournal of Lipid Research0022-22751969-09-01105495503Fecal neutral steroids and bile acids from germfree ratsThomas F. Kellogg0Bernard S. Wostmann1Lobund Laboratory and Department of Microbiology, Biochemistry and Biophysics Program, University of Notre Dame, Notre Dame, Indiana 46556Lobund Laboratory and Department of Microbiology, Biochemistry and Biophysics Program, University of Notre Dame, Notre Dame, Indiana 46556The amount and composition of fecal neutral sterols and bile acids excreted by adult male germfree and conventional rats have been determined. The amounts of neutral sterols excreted were 12.8 (germfree) and 19.5 (conventional) mg/kg of body wt per day. The germfree rats excreted cholesterol and lathosterol (methostenol was not assayed); the conventional rats excreted coprostanol and coprostanone in addition. The amounts of bile acids excreted were 11.3 (germfree) and 21.4 (conventional) mg/kg of body wt per day. The bile acids excreted by the rats were tentatively identified as tauro-β-muricholate, tauro-α-muricholate, and tauro-cholate, besides an unidentified component. The conventional rats excreted the corresponding unconjugated acids as well as many other unconjugated bile acids.No significant correlation was found between the amount of coprosterols and the total amount of neutral sterols excreted by the conventional rats. This suggests that bacterial reduction of cholesterol is not an important mechanism of increasing neutral sterol excretion of conventional rats as compared to germfree rats. Evidence is presented that suggests that this difference in neutral sterol excretion is due to changes in intestinal secretion and sloughing between the two types of animal. The factors reponsible for the differences in bile acid excretion have not been identified.http://www.sciencedirect.com/science/article/pii/S0022227520430408coprosterolssteroid balanceintestinal microfloracholesterollathosteroldehydroxylation |
| spellingShingle | Thomas F. Kellogg Bernard S. Wostmann Fecal neutral steroids and bile acids from germfree rats Journal of Lipid Research coprosterols steroid balance intestinal microflora cholesterol lathosterol dehydroxylation |
| title | Fecal neutral steroids and bile acids from germfree rats |
| title_full | Fecal neutral steroids and bile acids from germfree rats |
| title_fullStr | Fecal neutral steroids and bile acids from germfree rats |
| title_full_unstemmed | Fecal neutral steroids and bile acids from germfree rats |
| title_short | Fecal neutral steroids and bile acids from germfree rats |
| title_sort | fecal neutral steroids and bile acids from germfree rats |
| topic | coprosterols steroid balance intestinal microflora cholesterol lathosterol dehydroxylation |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520430408 |
| work_keys_str_mv | AT thomasfkellogg fecalneutralsteroidsandbileacidsfromgermfreerats AT bernardswostmann fecalneutralsteroidsandbileacidsfromgermfreerats |