Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering
<p>Abstract</p> <p>Background</p> <p>Tissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comp...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-12-01
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| Series: | BioMedical Engineering OnLine |
| Subjects: | |
| Online Access: | http://www.biomedical-engineering-online.com/content/11/1/92 |
| _version_ | 1818531067521400832 |
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| author | Aleksieva Genoveva Hollweck Trixi Thierfelder Nikolaus Haas Ulrike Koenig Fabian Fano Cornelia Dauner Martin Wintermantel Erich Reichart Bruno Schmitz Christoph Akra Bassil |
| author_facet | Aleksieva Genoveva Hollweck Trixi Thierfelder Nikolaus Haas Ulrike Koenig Fabian Fano Cornelia Dauner Martin Wintermantel Erich Reichart Bruno Schmitz Christoph Akra Bassil |
| author_sort | Aleksieva Genoveva |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods.</p> <p>Methods</p> <p>Human vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different cultivation methods of cell layers (A: static, B: dynamic). Group A was statically cultivated for 6 days. Group B was exposed to low flow conditions (t<sub>1</sub>= 3 days at 750 ml/min, t<sub>2</sub>= 2 days at 1100 ml/min) in a newly developed conditioning bioreactor. Samples were taken after static and dynamic cultivation and were analyzed by scanning electron microscopy (SEM), immunohistochemistry (IHC), and real time polymerase chain reaction (RT-PCR).</p> <p>Results</p> <p>SEM results showed a high density of adherent cells on the surface valves from both groups. However, better cell distribution and cell behavior was detected in Group B. IHC staining against CD31 and TE-7 revealed a positive reaction in both groups. Higher expression of extracellular matrix (ICAM, Collagen IV) was observed in Group B. RT- PCR demonstrated a higher expression of inflammatory Cytokines in Group B.</p> <p>Conclusion</p> <p>While conventional cultivation method can be used for the development of tissue engineered heart valves. Better results can be obtained by performing a conditioning step that may improve the tolerance of cells to shear stress. The novel pulsatile bioreactor offers an adequate tool for in vitro improvement of mechanical properties of tissue engineered cardiovascular prostheses.</p> |
| first_indexed | 2024-12-11T17:27:40Z |
| format | Article |
| id | doaj.art-eb3ff9a12e4e4ffe840391da60c0cdb5 |
| institution | Directory Open Access Journal |
| issn | 1475-925X |
| language | English |
| last_indexed | 2024-12-11T17:27:40Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj.art-eb3ff9a12e4e4ffe840391da60c0cdb52022-12-22T00:56:56ZengBMCBioMedical Engineering OnLine1475-925X2012-12-011119210.1186/1475-925X-11-92Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineeringAleksieva GenovevaHollweck TrixiThierfelder NikolausHaas UlrikeKoenig FabianFano CorneliaDauner MartinWintermantel ErichReichart BrunoSchmitz ChristophAkra Bassil<p>Abstract</p> <p>Background</p> <p>Tissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods.</p> <p>Methods</p> <p>Human vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different cultivation methods of cell layers (A: static, B: dynamic). Group A was statically cultivated for 6 days. Group B was exposed to low flow conditions (t<sub>1</sub>= 3 days at 750 ml/min, t<sub>2</sub>= 2 days at 1100 ml/min) in a newly developed conditioning bioreactor. Samples were taken after static and dynamic cultivation and were analyzed by scanning electron microscopy (SEM), immunohistochemistry (IHC), and real time polymerase chain reaction (RT-PCR).</p> <p>Results</p> <p>SEM results showed a high density of adherent cells on the surface valves from both groups. However, better cell distribution and cell behavior was detected in Group B. IHC staining against CD31 and TE-7 revealed a positive reaction in both groups. Higher expression of extracellular matrix (ICAM, Collagen IV) was observed in Group B. RT- PCR demonstrated a higher expression of inflammatory Cytokines in Group B.</p> <p>Conclusion</p> <p>While conventional cultivation method can be used for the development of tissue engineered heart valves. Better results can be obtained by performing a conditioning step that may improve the tolerance of cells to shear stress. The novel pulsatile bioreactor offers an adequate tool for in vitro improvement of mechanical properties of tissue engineered cardiovascular prostheses.</p>http://www.biomedical-engineering-online.com/content/11/1/92Tissue engineeringHeart valvePolyurethane scaffoldStatic cultivationDynamic cultivation |
| spellingShingle | Aleksieva Genoveva Hollweck Trixi Thierfelder Nikolaus Haas Ulrike Koenig Fabian Fano Cornelia Dauner Martin Wintermantel Erich Reichart Bruno Schmitz Christoph Akra Bassil Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering BioMedical Engineering OnLine Tissue engineering Heart valve Polyurethane scaffold Static cultivation Dynamic cultivation |
| title | Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| title_full | Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| title_fullStr | Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| title_full_unstemmed | Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| title_short | Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| title_sort | use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering |
| topic | Tissue engineering Heart valve Polyurethane scaffold Static cultivation Dynamic cultivation |
| url | http://www.biomedical-engineering-online.com/content/11/1/92 |
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