Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile
IntroductionAntimicrobial resistance (AMR) is a major threat to animal and public health worldwide; consequently, several AMR surveillances programs have been implemented internationally in both human and veterinary medicine, including indicator bacteria such as Escherichia coli. However, companion...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1233127/full |
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| author | Nicolás Galarce Gabriel Arriagada Fernando Sánchez Fernando Sánchez Beatriz Escobar Mauricio Miranda Sofía Matus Rocío Vilches Camila Varela Carlos Zelaya Josefa Peralta Esteban Paredes-Osses Esteban Paredes-Osses Gerardo González-Rocha Lisette Lapierre |
| author_facet | Nicolás Galarce Gabriel Arriagada Fernando Sánchez Fernando Sánchez Beatriz Escobar Mauricio Miranda Sofía Matus Rocío Vilches Camila Varela Carlos Zelaya Josefa Peralta Esteban Paredes-Osses Esteban Paredes-Osses Gerardo González-Rocha Lisette Lapierre |
| author_sort | Nicolás Galarce |
| collection | DOAJ |
| description | IntroductionAntimicrobial resistance (AMR) is a major threat to animal and public health worldwide; consequently, several AMR surveillances programs have been implemented internationally in both human and veterinary medicine, including indicator bacteria such as Escherichia coli. However, companion animals are not typically included in these surveillance programs. Nevertheless, there have been reports of increasing levels of antimicrobial resistance in E. coli strains isolated from dogs worldwide. In Chile, there is limited information available on AMR in E. coli isolated from companion animals, which prevents the establishment of objective prevention and control measures.MethodsFor this reason, the aim of this study was to characterize the phenotypic and genotypic AMR of E. coli strains isolated from healthy household dogs in Chile. For this purpose, a multi-stage sampling was carried out in the Metropolitan Region of Chile, obtaining samples from 600 healthy dogs. These samples were processed using traditional bacteriology and molecular techniques to isolate E. coli strains. We assessed the minimal inhibitory concentration of 17 antimicrobials and conducted a search of six antimicrobial resistance genes, as well as class 1 and 2 integrons, in the isolated strains.ResultsTwo-hundred and twenty-four strains of E. coli were recovered, and 96.9% (n = 217) showed resistance to at least one drug and only 3.1% (n = 7) were susceptible to all analyzed antimicrobials. Most strains were resistant to cefalexin (91.5%, n = 205, 1st-generation cephalosporin), followed by ampicillin (68.3%, n = 153) and cefpodoxime (31.3%, n = 70, 3rd-generation cephalosporin). Moreover, 24.1% (n = 54) tested positive for extended-spectrum-β-lactamases and 34.4% (n = 77) were multidrug resistant. As for the AMR genes, the most detected was qnrB (28.1%, n = 63), followed by blaCTX-M (22.3%, n = 50), and blaTEM-1 (19.6%, n = 44). Additionally, 16.1% (n = 36) harbored class 1 integrons. Our study shows that E. coli strains isolated from healthy household dogs exhibit resistance to several relevant drugs and also antimicrobial resistance genes considered critical for human health. These results can be used as a starting point for the prevention and control of antimicrobial resistance from companion animals. This background should be considered when formulating future resistance surveillance programs or control plans in which companion animals must be included. |
| first_indexed | 2024-03-12T14:25:49Z |
| format | Article |
| id | doaj.art-eb41b5296bea46dbaaeae072ead73f6a |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T14:25:49Z |
| publishDate | 2023-08-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-eb41b5296bea46dbaaeae072ead73f6a2023-08-18T08:25:55ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-08-011010.3389/fvets.2023.12331271233127Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in ChileNicolás Galarce0Gabriel Arriagada1Fernando Sánchez2Fernando Sánchez3Beatriz Escobar4Mauricio Miranda5Sofía Matus6Rocío Vilches7Camila Varela8Carlos Zelaya9Josefa Peralta10Esteban Paredes-Osses11Esteban Paredes-Osses12Gerardo González-Rocha13Lisette Lapierre14Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando, ChilePrograma de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Salud Ambiental, Instituto de Salud Pública de Chile, Santiago, ChileInstituto de Ciencias Naturales, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Providencia, ChileLaboratorio de Investigación en Agentes Antibacterianos, Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileIntroductionAntimicrobial resistance (AMR) is a major threat to animal and public health worldwide; consequently, several AMR surveillances programs have been implemented internationally in both human and veterinary medicine, including indicator bacteria such as Escherichia coli. However, companion animals are not typically included in these surveillance programs. Nevertheless, there have been reports of increasing levels of antimicrobial resistance in E. coli strains isolated from dogs worldwide. In Chile, there is limited information available on AMR in E. coli isolated from companion animals, which prevents the establishment of objective prevention and control measures.MethodsFor this reason, the aim of this study was to characterize the phenotypic and genotypic AMR of E. coli strains isolated from healthy household dogs in Chile. For this purpose, a multi-stage sampling was carried out in the Metropolitan Region of Chile, obtaining samples from 600 healthy dogs. These samples were processed using traditional bacteriology and molecular techniques to isolate E. coli strains. We assessed the minimal inhibitory concentration of 17 antimicrobials and conducted a search of six antimicrobial resistance genes, as well as class 1 and 2 integrons, in the isolated strains.ResultsTwo-hundred and twenty-four strains of E. coli were recovered, and 96.9% (n = 217) showed resistance to at least one drug and only 3.1% (n = 7) were susceptible to all analyzed antimicrobials. Most strains were resistant to cefalexin (91.5%, n = 205, 1st-generation cephalosporin), followed by ampicillin (68.3%, n = 153) and cefpodoxime (31.3%, n = 70, 3rd-generation cephalosporin). Moreover, 24.1% (n = 54) tested positive for extended-spectrum-β-lactamases and 34.4% (n = 77) were multidrug resistant. As for the AMR genes, the most detected was qnrB (28.1%, n = 63), followed by blaCTX-M (22.3%, n = 50), and blaTEM-1 (19.6%, n = 44). Additionally, 16.1% (n = 36) harbored class 1 integrons. Our study shows that E. coli strains isolated from healthy household dogs exhibit resistance to several relevant drugs and also antimicrobial resistance genes considered critical for human health. These results can be used as a starting point for the prevention and control of antimicrobial resistance from companion animals. This background should be considered when formulating future resistance surveillance programs or control plans in which companion animals must be included.https://www.frontiersin.org/articles/10.3389/fvets.2023.1233127/fullantimicrobial resistanceEscherichia colidogsdrug resistancegenotypicphenotypic |
| spellingShingle | Nicolás Galarce Gabriel Arriagada Fernando Sánchez Fernando Sánchez Beatriz Escobar Mauricio Miranda Sofía Matus Rocío Vilches Camila Varela Carlos Zelaya Josefa Peralta Esteban Paredes-Osses Esteban Paredes-Osses Gerardo González-Rocha Lisette Lapierre Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile Frontiers in Veterinary Science antimicrobial resistance Escherichia coli dogs drug resistance genotypic phenotypic |
| title | Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile |
| title_full | Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile |
| title_fullStr | Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile |
| title_full_unstemmed | Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile |
| title_short | Phenotypic and genotypic antimicrobial resistance in Escherichia coli strains isolated from household dogs in Chile |
| title_sort | phenotypic and genotypic antimicrobial resistance in escherichia coli strains isolated from household dogs in chile |
| topic | antimicrobial resistance Escherichia coli dogs drug resistance genotypic phenotypic |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1233127/full |
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