The first genistin absorption screening into vacuoles of Trifolium pratense L.
The determination of a transport mechanism for genistin (genistein-7-O-glucoside) across the tonoplast was performed on vacuoles from a cell culture of Trifolium pratense L. Genistin levels were examined in vacuoles as well as in an assay medium by HPLC (high-performance liquid chromatography) after...
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| Language: | English |
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Czech Academy of Agricultural Sciences
2018-06-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-201806-0007_the-first-genistin-absorption-screening-into-vacuoles-of-trifolium-pratense-l.php |
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| author | Jan KUBEŠ Milan SKALICKÝ Václav HEJNÁK Lenka TŮMOVÁ Jan MARTIN Jaroslava MARTINKOVÁ |
| author_facet | Jan KUBEŠ Milan SKALICKÝ Václav HEJNÁK Lenka TŮMOVÁ Jan MARTIN Jaroslava MARTINKOVÁ |
| author_sort | Jan KUBEŠ |
| collection | DOAJ |
| description | The determination of a transport mechanism for genistin (genistein-7-O-glucoside) across the tonoplast was performed on vacuoles from a cell culture of Trifolium pratense L. Genistin levels were examined in vacuoles as well as in an assay medium by HPLC (high-performance liquid chromatography) after treatment with various substances. MgATP increased the uptake of added genistin by 25%, but the nucleotide-free samples also contained this glycoside. Applying bafilomycin A1, an H+-ATPase inhibitor, indistinctly inhibited genistin absorption. However, vacuolar absorption of genistin was significantly reduced by N,N´-dicyclohexylcarbodiimide. This inhibitor can suppress both H+-ATPase and H+-PPase; the effect of pyrophosphate alone was not investigated. An increase in genistein levels, as result of genistin hydrolysis, could also affect the transport mechanism. The results investigated with inhibitors suggest the possible involvement of proton pumps; however additional research is required to confirm the participation of multidrug and toxin extrusion (MATE) proteins in genistin transport. |
| first_indexed | 2024-04-10T08:09:07Z |
| format | Article |
| id | doaj.art-eb44aefb097c4a99a8d58ba93176be76 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:09:07Z |
| publishDate | 2018-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-eb44aefb097c4a99a8d58ba93176be762023-02-23T03:46:39ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682018-06-0164629029610.17221/134/2018-PSEpse-201806-0007The first genistin absorption screening into vacuoles of Trifolium pratense L.Jan KUBEŠ0Milan SKALICKÝ1Václav HEJNÁK2Lenka TŮMOVÁ3Jan MARTIN4Jaroslava MARTINKOVÁ5Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicFaculty of Pharmacy in Hradec Králové, Charles University, Prague, Czech RepublicFaculty of Pharmacy in Hradec Králové, Charles University, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicThe determination of a transport mechanism for genistin (genistein-7-O-glucoside) across the tonoplast was performed on vacuoles from a cell culture of Trifolium pratense L. Genistin levels were examined in vacuoles as well as in an assay medium by HPLC (high-performance liquid chromatography) after treatment with various substances. MgATP increased the uptake of added genistin by 25%, but the nucleotide-free samples also contained this glycoside. Applying bafilomycin A1, an H+-ATPase inhibitor, indistinctly inhibited genistin absorption. However, vacuolar absorption of genistin was significantly reduced by N,N´-dicyclohexylcarbodiimide. This inhibitor can suppress both H+-ATPase and H+-PPase; the effect of pyrophosphate alone was not investigated. An increase in genistein levels, as result of genistin hydrolysis, could also affect the transport mechanism. The results investigated with inhibitors suggest the possible involvement of proton pumps; however additional research is required to confirm the participation of multidrug and toxin extrusion (MATE) proteins in genistin transport.https://pse.agriculturejournals.cz/artkey/pse-201806-0007_the-first-genistin-absorption-screening-into-vacuoles-of-trifolium-pratense-l.phpred cloverin vitro cultureisoflavonesmembrane transportphytoestrogen |
| spellingShingle | Jan KUBEŠ Milan SKALICKÝ Václav HEJNÁK Lenka TŮMOVÁ Jan MARTIN Jaroslava MARTINKOVÁ The first genistin absorption screening into vacuoles of Trifolium pratense L. Plant, Soil and Environment red clover in vitro culture isoflavones membrane transport phytoestrogen |
| title | The first genistin absorption screening into vacuoles of Trifolium pratense L. |
| title_full | The first genistin absorption screening into vacuoles of Trifolium pratense L. |
| title_fullStr | The first genistin absorption screening into vacuoles of Trifolium pratense L. |
| title_full_unstemmed | The first genistin absorption screening into vacuoles of Trifolium pratense L. |
| title_short | The first genistin absorption screening into vacuoles of Trifolium pratense L. |
| title_sort | first genistin absorption screening into vacuoles of trifolium pratense l |
| topic | red clover in vitro culture isoflavones membrane transport phytoestrogen |
| url | https://pse.agriculturejournals.cz/artkey/pse-201806-0007_the-first-genistin-absorption-screening-into-vacuoles-of-trifolium-pratense-l.php |
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