lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling
Uncontrolled growth and an enforced epithelial-mesenchymal transition (EMT) process contribute to the poor survival rate of patients with osteosarcoma (OS). Long noncoding RNAs (lncRNAs) have been reported to be involved in the development of OS. However, the significant role of lncRNA SNHG1O on reg...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S216225312030322X |
| _version_ | 1819056555242291200 |
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| author | Shutao Zhu Yang Liu Xiao Wang Junyi Wang Guanghui Xi |
| author_facet | Shutao Zhu Yang Liu Xiao Wang Junyi Wang Guanghui Xi |
| author_sort | Shutao Zhu |
| collection | DOAJ |
| description | Uncontrolled growth and an enforced epithelial-mesenchymal transition (EMT) process contribute to the poor survival rate of patients with osteosarcoma (OS). Long noncoding RNAs (lncRNAs) have been reported to be involved in the development of OS. However, the significant role of lncRNA SNHG1O on regulating proliferation and the EMT process of OS cells remains unclear. In this study, quantitative real-time PCR and fluorescence in situ hybridization (FISH) results suggested that SNHG10 levels were significantly increased in OS compared with healthy tissues. In vitro experiments (including colony formation, CCK-8, wound healing, and transwell assays) and in vivo experiments indicated that downregulation of SNHG10 significantly suppressed the proliferation and invasion of OS cells. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay confirmed that SNHG10 could regulate FZD3 levels through sponging microRNA 182-5p (miR-182-5p). In addition, the SNHG10/miR-182-5p/FZD3 axis could further promote the β-catenin transfer into nuclear accumulation to maintain the activation of the Wnt singling pathway. Together, our results established that SNHG10 has an important role in promoting OS growth and invasion. By sponging miR-182-5p, SNHG10 can increase FZD3 expression and further maintain the activation of Wnt/β-catenin singling pathway in OS cells. |
| first_indexed | 2024-12-21T13:25:16Z |
| format | Article |
| id | doaj.art-eb4b3a5661ba4bc092b5c7ca06dfb57b |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-21T13:25:16Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-eb4b3a5661ba4bc092b5c7ca06dfb57b2022-12-21T19:02:28ZengElsevierMolecular Therapy: Nucleic Acids2162-25312020-12-0122957970lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin SignalingShutao Zhu0Yang Liu1Xiao Wang2Junyi Wang3Guanghui Xi4Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, ChinaDepartment of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, China; Corresponding author: Yang Liu, Department of orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, China.Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, ChinaDepartment of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, ChinaDepartment of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, Henan, ChinaUncontrolled growth and an enforced epithelial-mesenchymal transition (EMT) process contribute to the poor survival rate of patients with osteosarcoma (OS). Long noncoding RNAs (lncRNAs) have been reported to be involved in the development of OS. However, the significant role of lncRNA SNHG1O on regulating proliferation and the EMT process of OS cells remains unclear. In this study, quantitative real-time PCR and fluorescence in situ hybridization (FISH) results suggested that SNHG10 levels were significantly increased in OS compared with healthy tissues. In vitro experiments (including colony formation, CCK-8, wound healing, and transwell assays) and in vivo experiments indicated that downregulation of SNHG10 significantly suppressed the proliferation and invasion of OS cells. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay confirmed that SNHG10 could regulate FZD3 levels through sponging microRNA 182-5p (miR-182-5p). In addition, the SNHG10/miR-182-5p/FZD3 axis could further promote the β-catenin transfer into nuclear accumulation to maintain the activation of the Wnt singling pathway. Together, our results established that SNHG10 has an important role in promoting OS growth and invasion. By sponging miR-182-5p, SNHG10 can increase FZD3 expression and further maintain the activation of Wnt/β-catenin singling pathway in OS cells.http://www.sciencedirect.com/science/article/pii/S216225312030322XosteosarcomaSNHG10Wnt/β-cateninlncRNAEMT |
| spellingShingle | Shutao Zhu Yang Liu Xiao Wang Junyi Wang Guanghui Xi lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling Molecular Therapy: Nucleic Acids osteosarcoma SNHG10 Wnt/β-catenin lncRNA EMT |
| title | lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling |
| title_full | lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling |
| title_fullStr | lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling |
| title_full_unstemmed | lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling |
| title_short | lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling |
| title_sort | lncrna snhg10 promotes the proliferation and invasion of osteosarcoma via wnt β catenin signaling |
| topic | osteosarcoma SNHG10 Wnt/β-catenin lncRNA EMT |
| url | http://www.sciencedirect.com/science/article/pii/S216225312030322X |
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