Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications
Triphala is one of the commonly used ayurvedic formulations for gastrointestinal disorders. In Ayurveda, Triphala water has been used to wash wounds as an antimicrobial agent for wound healing. In this study, the wound healing property of Triphala was further enhanced by mixing equal proportions of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Results in Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590048X23000456 |
| _version_ | 1797804631000088576 |
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| author | Rajalakshmi Ramamoorthy Swetha Andra Satheesh kumar Balu Fouad Damiri Gopi Krishnan N Muthumanickkam Andiappan Murugesan Muthalagu Mohammed Berrada |
| author_facet | Rajalakshmi Ramamoorthy Swetha Andra Satheesh kumar Balu Fouad Damiri Gopi Krishnan N Muthumanickkam Andiappan Murugesan Muthalagu Mohammed Berrada |
| author_sort | Rajalakshmi Ramamoorthy |
| collection | DOAJ |
| description | Triphala is one of the commonly used ayurvedic formulations for gastrointestinal disorders. In Ayurveda, Triphala water has been used to wash wounds as an antimicrobial agent for wound healing. In this study, the wound healing property of Triphala was further enhanced by mixing equal proportions of medicinal plants like Acacia catechu, Glycyrrhiza glabra Azadirachta indica, and Coscinium fenestratum. The polyherbal extract was prepared from the new polyherbal formulation using a methanol-chloroform solvent mixture and analysed for its quality and quantity of phytochemicals. Then, the polyherbal extract at 1%, 3%, and 5% concentration was mixed with 10% polycaprolactone (PCL) polymer and converted into poly-herbal extract-loaded nanofibers using an electrospinning technique. The prepared nanofibers were characterized by FTIR, XRD, FESEM, contact angle, and water absorption. Further, the biological properties like antimicrobial activity and biocompatibility were also analysed. Finally, the in-vivo wound healing efficiency was studied using an animal model. The FTIR analysis reveals the presence of unique phytochemicals of plant extracts. Further, the FESEM confirms the presence of the drug on the surface of nanofibers. From the XRD, the reduction in crystallinity was observed due to herbal drug incorporation. Further, contact angle, water absorption also proved the absorbing ability of polyherbal-loaded nanofibers. The polycaprolactone-polyherbal (PCL-PHF) formulation of 3% and 5% loaded nanofibers shows faster wound healing compared to the control and standard. In addition, the polyherbal drug-loaded polycaprolactone shows faster granulation tissue formation and collagen synthesis compared to the standard group. The antioxidant property of the patch protects the wound from lipid peroxides and myeloperoxidase. Overall, the antimicrobial, antioxidant, and anti-inflammatory property of polyherbal extract aids in faster wound healing. This finding suggests that the polyherbal drug-loaded polycaprolactone nanofibrous mats could be used as an effective candidate for the treatment of open wounds. |
| first_indexed | 2024-03-13T05:40:03Z |
| format | Article |
| id | doaj.art-eb4b7d69f890463694d4ef704582397d |
| institution | Directory Open Access Journal |
| issn | 2590-048X |
| language | English |
| last_indexed | 2024-03-13T05:40:03Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Materials |
| spelling | doaj.art-eb4b7d69f890463694d4ef704582397d2023-06-14T04:34:05ZengElsevierResults in Materials2590-048X2023-06-0118100407Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applicationsRajalakshmi Ramamoorthy0Swetha Andra1Satheesh kumar Balu2Fouad Damiri3Gopi Krishnan N4Muthumanickkam Andiappan5Murugesan Muthalagu6Mohammed Berrada7Department of Textile Technology, Anna University, Chennai, 600025, Tamil Nadu, IndiaDepartment of Chemistry, Rajalakshmi Institute of Technology, Chennai, 600124 Tamil Nadu, IndiaDepartment of Oral Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, Tamil Nadu, IndiaLaboratory of Biomolecules and Organic Synthesis (BIOSYNTHO), Department of Chemistry, Faculty of Sciences Ben M'Sick, University Hassan II of Casablanca, Casablanca 20000, Morocco; Corresponding author.Department of Physics, Indian Institute of Technology Madras, Chennai, 600036, Tamil Nadu, IndiaDepartment of Textile Technology, Anna University, Chennai, 600025, Tamil Nadu, IndiaDepartment of Textile Technology, Anna University, Chennai, 600025, Tamil Nadu, India; Corresponding author.Laboratory of Biomolecules and Organic Synthesis (BIOSYNTHO), Department of Chemistry, Faculty of Sciences Ben M'Sick, University Hassan II of Casablanca, Casablanca 20000, MoroccoTriphala is one of the commonly used ayurvedic formulations for gastrointestinal disorders. In Ayurveda, Triphala water has been used to wash wounds as an antimicrobial agent for wound healing. In this study, the wound healing property of Triphala was further enhanced by mixing equal proportions of medicinal plants like Acacia catechu, Glycyrrhiza glabra Azadirachta indica, and Coscinium fenestratum. The polyherbal extract was prepared from the new polyherbal formulation using a methanol-chloroform solvent mixture and analysed for its quality and quantity of phytochemicals. Then, the polyherbal extract at 1%, 3%, and 5% concentration was mixed with 10% polycaprolactone (PCL) polymer and converted into poly-herbal extract-loaded nanofibers using an electrospinning technique. The prepared nanofibers were characterized by FTIR, XRD, FESEM, contact angle, and water absorption. Further, the biological properties like antimicrobial activity and biocompatibility were also analysed. Finally, the in-vivo wound healing efficiency was studied using an animal model. The FTIR analysis reveals the presence of unique phytochemicals of plant extracts. Further, the FESEM confirms the presence of the drug on the surface of nanofibers. From the XRD, the reduction in crystallinity was observed due to herbal drug incorporation. Further, contact angle, water absorption also proved the absorbing ability of polyherbal-loaded nanofibers. The polycaprolactone-polyherbal (PCL-PHF) formulation of 3% and 5% loaded nanofibers shows faster wound healing compared to the control and standard. In addition, the polyherbal drug-loaded polycaprolactone shows faster granulation tissue formation and collagen synthesis compared to the standard group. The antioxidant property of the patch protects the wound from lipid peroxides and myeloperoxidase. Overall, the antimicrobial, antioxidant, and anti-inflammatory property of polyherbal extract aids in faster wound healing. This finding suggests that the polyherbal drug-loaded polycaprolactone nanofibrous mats could be used as an effective candidate for the treatment of open wounds.http://www.sciencedirect.com/science/article/pii/S2590048X23000456Wound careMedicinal plantsElectrospinningAntibacterialAntioxidants |
| spellingShingle | Rajalakshmi Ramamoorthy Swetha Andra Satheesh kumar Balu Fouad Damiri Gopi Krishnan N Muthumanickkam Andiappan Murugesan Muthalagu Mohammed Berrada Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications Results in Materials Wound care Medicinal plants Electrospinning Antibacterial Antioxidants |
| title | Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications |
| title_full | Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications |
| title_fullStr | Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications |
| title_full_unstemmed | Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications |
| title_short | Flavonoids, phenolics, and tannins loaded polycaprolactone nanofibers (NF) for wound dressing applications |
| title_sort | flavonoids phenolics and tannins loaded polycaprolactone nanofibers nf for wound dressing applications |
| topic | Wound care Medicinal plants Electrospinning Antibacterial Antioxidants |
| url | http://www.sciencedirect.com/science/article/pii/S2590048X23000456 |
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