Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery
Abstract Background The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation bas...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-017-0317-y |
| _version_ | 1798041234683461632 |
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| author | Qingtong Yu Jingjing Chen Wenwen Deng Xia Cao Yan Wang Jie Zhou Wenqian Xu Pan Du Qiang Wang Jiangnan Yu Ximing Xu |
| author_facet | Qingtong Yu Jingjing Chen Wenwen Deng Xia Cao Yan Wang Jie Zhou Wenqian Xu Pan Du Qiang Wang Jiangnan Yu Ximing Xu |
| author_sort | Qingtong Yu |
| collection | DOAJ |
| description | Abstract Background The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts. Results Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP–pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point. Conclusion Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries. |
| first_indexed | 2024-04-11T22:18:38Z |
| format | Article |
| id | doaj.art-eb4b8aa78a3241cfa700724ebd266622 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T22:18:38Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-eb4b8aa78a3241cfa700724ebd2666222022-12-22T04:00:17ZengBMCJournal of Nanobiotechnology1477-31552017-11-0115111510.1186/s12951-017-0317-yDirect reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-deliveryQingtong Yu0Jingjing Chen1Wenwen Deng2Xia Cao3Yan Wang4Jie Zhou5Wenqian Xu6Pan Du7Qiang Wang8Jiangnan Yu9Ximing Xu10Department of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityDepartment of Pharmaceutics, School of Pharmacy, and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu UniversityAbstract Background The cell source for transplantation therapy is always a prerequisite question to be solved in clinical applications. Neural cells are considered non-regenerable, which highly restrict their application in the treatment for nerve injury. Therefore, neural trans-differentiation based on gene transfection provides a new solution to this issue. Compared to viral strategy, non-viral gene delivery systems are considered as a more promising way to achieve this aim. This study centers on a novel application of Porphyra yezoensis polysaccharide as a non-viral gene carrier for the neural trans-differentiation of mouse fibroblasts. Results Ethanediamine modified P. yezoensis polysaccharide (Ed-PYP) served as a gene carrier and a group of plasmids that encode Ascl1, Brn4, and Tcf3 (pABT) self-assembled into nanoparticles. Results demonstrated that Ed-PYP–pABT nanoparticles at Ed-PYP: pABT weight ratio of 40:1 was the optimal candidate for gene delivery. ELISA assay revealed the highest expression levels of NGF, BDNF and SHH at 14 days after last transfection. Immunofluorescence and western blot assays also showed robust expression of neural markers including Nestin, GFAP, β-3tubulin, NF200, GAP43 and MAP2, in induced 3T6 cells at this time point. Conclusion Overall, these findings indicated that the P. yezoensis polysaccharide-based non-viral gene co-delivery system is a promising strategy for the generation of neural cells, which might facilitate the developments in the recovery of neural injuries.http://link.springer.com/article/10.1186/s12951-017-0317-yPorphyra yezoensisCationized polysaccharideGene co-delivery, nanoparticlesNanoparticlesNeural trans-differentiation |
| spellingShingle | Qingtong Yu Jingjing Chen Wenwen Deng Xia Cao Yan Wang Jie Zhou Wenqian Xu Pan Du Qiang Wang Jiangnan Yu Ximing Xu Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery Journal of Nanobiotechnology Porphyra yezoensis Cationized polysaccharide Gene co-delivery, nanoparticles Nanoparticles Neural trans-differentiation |
| title | Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery |
| title_full | Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery |
| title_fullStr | Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery |
| title_full_unstemmed | Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery |
| title_short | Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery |
| title_sort | direct reprogramming of mouse fibroblasts into neural cells via porphyra yezoensis polysaccharide based high efficient gene co delivery |
| topic | Porphyra yezoensis Cationized polysaccharide Gene co-delivery, nanoparticles Nanoparticles Neural trans-differentiation |
| url | http://link.springer.com/article/10.1186/s12951-017-0317-y |
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