The Association between Infant Colic and the Multi-Omic Composition of Human Milk
Infant colic is a common condition with unclear biologic underpinnings and limited treatment options. We hypothesized that complex molecular networks within human milk (i.e., microbes, micro-ribonucleic acids (miRNAs), cytokines) would contribute to colic risk, while controlling for medical, social,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/3/559 |
| _version_ | 1797613238751330304 |
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| author | Desirae Chandran Kaitlyn Warren Daniel McKeone Steven D. Hicks |
| author_facet | Desirae Chandran Kaitlyn Warren Daniel McKeone Steven D. Hicks |
| author_sort | Desirae Chandran |
| collection | DOAJ |
| description | Infant colic is a common condition with unclear biologic underpinnings and limited treatment options. We hypothesized that complex molecular networks within human milk (i.e., microbes, micro-ribonucleic acids (miRNAs), cytokines) would contribute to colic risk, while controlling for medical, social, and nutritional variables. This hypothesis was tested in a cohort of 182 breastfed infants, assessed with a modified Infant Colic Scale at 1 month. RNA sequencing was used to interrogate microbial and miRNA features. Luminex assays were used to measure growth factors and cytokines. Milk from mothers of infants with colic (<i>n</i> = 28) displayed higher levels of <i>Staphylococcus</i> (adj. <i>p</i> = 0.038, <i>d</i> = 0.30), miR-224-3p (adj. <i>p</i> = 0.023, <i>d</i> = 0.33), miR-125b-5p (adj. <i>p</i> = 0.028, <i>d</i> = 0.29), let-7a-5p (adj. <i>p</i> = 0.028, <i>d</i> = 0.27), and miR-205-5p (adj. <i>p</i> = 0.029, <i>d</i> = 0.26) compared to milk from non-colic mother–infant dyads (<i>n</i> = 154). Colic symptom severity was directly associated with milk hepatocyte growth factor levels (<i>R</i> = 0.21, <i>p</i> = 0.025). A regression model involving let-7a-5p, miR-29a-3p, and <i>Lactobacillus</i> accurately modeled colic risk (<i>X</i><sup>2</sup> = 16.7, <i>p</i> = 0.001). Molecular factors within human milk may impact colic risk, and provide support for a dysbiotic/inflammatory model of colic pathophysiology. |
| first_indexed | 2024-03-11T06:53:04Z |
| format | Article |
| id | doaj.art-eb4f2a95924b49648e237b439e2b2487 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T06:53:04Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-eb4f2a95924b49648e237b439e2b24872023-11-17T09:53:00ZengMDPI AGBiomolecules2218-273X2023-03-0113355910.3390/biom13030559The Association between Infant Colic and the Multi-Omic Composition of Human MilkDesirae Chandran0Kaitlyn Warren1Daniel McKeone2Steven D. Hicks3Department of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USAInfant colic is a common condition with unclear biologic underpinnings and limited treatment options. We hypothesized that complex molecular networks within human milk (i.e., microbes, micro-ribonucleic acids (miRNAs), cytokines) would contribute to colic risk, while controlling for medical, social, and nutritional variables. This hypothesis was tested in a cohort of 182 breastfed infants, assessed with a modified Infant Colic Scale at 1 month. RNA sequencing was used to interrogate microbial and miRNA features. Luminex assays were used to measure growth factors and cytokines. Milk from mothers of infants with colic (<i>n</i> = 28) displayed higher levels of <i>Staphylococcus</i> (adj. <i>p</i> = 0.038, <i>d</i> = 0.30), miR-224-3p (adj. <i>p</i> = 0.023, <i>d</i> = 0.33), miR-125b-5p (adj. <i>p</i> = 0.028, <i>d</i> = 0.29), let-7a-5p (adj. <i>p</i> = 0.028, <i>d</i> = 0.27), and miR-205-5p (adj. <i>p</i> = 0.029, <i>d</i> = 0.26) compared to milk from non-colic mother–infant dyads (<i>n</i> = 154). Colic symptom severity was directly associated with milk hepatocyte growth factor levels (<i>R</i> = 0.21, <i>p</i> = 0.025). A regression model involving let-7a-5p, miR-29a-3p, and <i>Lactobacillus</i> accurately modeled colic risk (<i>X</i><sup>2</sup> = 16.7, <i>p</i> = 0.001). Molecular factors within human milk may impact colic risk, and provide support for a dysbiotic/inflammatory model of colic pathophysiology.https://www.mdpi.com/2218-273X/13/3/559<i>Lactobacillus</i>colicbreastmilkmicrobiomecytokinesmicroRNA |
| spellingShingle | Desirae Chandran Kaitlyn Warren Daniel McKeone Steven D. Hicks The Association between Infant Colic and the Multi-Omic Composition of Human Milk Biomolecules <i>Lactobacillus</i> colic breastmilk microbiome cytokines microRNA |
| title | The Association between Infant Colic and the Multi-Omic Composition of Human Milk |
| title_full | The Association between Infant Colic and the Multi-Omic Composition of Human Milk |
| title_fullStr | The Association between Infant Colic and the Multi-Omic Composition of Human Milk |
| title_full_unstemmed | The Association between Infant Colic and the Multi-Omic Composition of Human Milk |
| title_short | The Association between Infant Colic and the Multi-Omic Composition of Human Milk |
| title_sort | association between infant colic and the multi omic composition of human milk |
| topic | <i>Lactobacillus</i> colic breastmilk microbiome cytokines microRNA |
| url | https://www.mdpi.com/2218-273X/13/3/559 |
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