Isolation and validation of an endogenous fluorescent nucleoid reporter in Salmonella Typhimurium.

Isolation and validation of an endogenous fluorescent nucleoid reporter in Salmonella Typhimurium.

In this study we adapted a Mud-based delivery system to construct a random yfp reporter gene (encoding the yellow fluorescent protein) insertion library in the genome of Salmonella Typhimurium LT2, and used fluorescence activated cell sorting and fluorescence microscopy to screen for translational f...

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Bibliographic Details
Main Authors: Ioannis Passaris, Anirban Ghosh, William Cenens, Chris W Michiels, Jeroen Lammertyn, Abram Aertsen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3973593?pdf=render

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