Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes
Eco-friendly new mosquito control innovations are critical for the ongoing success of global mosquito control programs. In this study, Sh.463_56.10R, a robust RNA interference (RNAi) yeast insecticide strain that is suitable for scaled fermentation, was evaluated under semi-field conditions. Inactiv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/12/950 |
| _version_ | 1797380652221333504 |
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| author | Akilah T. M. Stewart Keshava Mysore Teresia M. Njoroge Nikhella Winter Rachel Shui Feng Satish Singh Lester D. James Preeraya Singkhaimuk Longhua Sun Azad Mohammed James D. Oxley Craig Duckham Alongkot Ponlawat David W. Severson Molly Duman-Scheel |
| author_facet | Akilah T. M. Stewart Keshava Mysore Teresia M. Njoroge Nikhella Winter Rachel Shui Feng Satish Singh Lester D. James Preeraya Singkhaimuk Longhua Sun Azad Mohammed James D. Oxley Craig Duckham Alongkot Ponlawat David W. Severson Molly Duman-Scheel |
| author_sort | Akilah T. M. Stewart |
| collection | DOAJ |
| description | Eco-friendly new mosquito control innovations are critical for the ongoing success of global mosquito control programs. In this study, Sh.463_56.10R, a robust RNA interference (RNAi) yeast insecticide strain that is suitable for scaled fermentation, was evaluated under semi-field conditions. Inactivated and dried Sh.463_56.10R yeast induced significant mortality of field strain <i>Aedes aegypti</i>, <i>Aedes albopictus</i>, and <i>Culex quinquefasciatus</i> larvae in semi-field larvicide trials conducted outdoors in St. Augustine, Trinidad, where 100% of the larvae were dead within 24 h. The yeast was also stably suspended in commercial bait and deployed as an active ingredient in miniature attractive targeted sugar bait (ATSB) station sachets. The yeast ATSB induced high levels of <i>Aedes</i> and <i>Culex</i> mosquito morbidity in semi-field trials conducted in Trinidad, West Indies, as well as in Bangkok, Thailand, in which the consumption of the yeast resulted in adult female mosquito death within 48 h, faster than what was observed in laboratory trials. These findings support the pursuit of large-scale field trials to further evaluate the Sh.463_56.10R insecticide, a member of a promising new class of species-specific RNAi insecticides that could help combat insecticide resistance and support effective mosquito control programs worldwide. |
| first_indexed | 2024-03-08T20:40:19Z |
| format | Article |
| id | doaj.art-eb501614b0b74eda845fc4bc58a4cd0b |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-08T20:40:19Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-eb501614b0b74eda845fc4bc58a4cd0b2023-12-22T14:16:19ZengMDPI AGInsects2075-44502023-12-01141295010.3390/insects14120950Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> MosquitoesAkilah T. M. Stewart0Keshava Mysore1Teresia M. Njoroge2Nikhella Winter3Rachel Shui Feng4Satish Singh5Lester D. James6Preeraya Singkhaimuk7Longhua Sun8Azad Mohammed9James D. Oxley10Craig Duckham11Alongkot Ponlawat12David W. Severson13Molly Duman-Scheel14Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USADepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USADepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USADepartment of Life Sciences, Faculty of Science & Technology, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and TobagoDepartment of Life Sciences, Faculty of Science & Technology, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and TobagoDepartment of Life Sciences, Faculty of Science & Technology, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and TobagoDepartment of Life Sciences, Faculty of Science & Technology, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and TobagoDepartment of Entomology, US Army Medical Directorate–Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS), Bangkok 10400, ThailandDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USADepartment of Life Sciences, Faculty of Science & Technology, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and TobagoSouthwest Research Institute, San Antonio, TX 78238, USACD R&D Consultancy Services, Dorking RH4 2HF, UKDepartment of Entomology, US Army Medical Directorate–Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS), Bangkok 10400, ThailandDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USADepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USAEco-friendly new mosquito control innovations are critical for the ongoing success of global mosquito control programs. In this study, Sh.463_56.10R, a robust RNA interference (RNAi) yeast insecticide strain that is suitable for scaled fermentation, was evaluated under semi-field conditions. Inactivated and dried Sh.463_56.10R yeast induced significant mortality of field strain <i>Aedes aegypti</i>, <i>Aedes albopictus</i>, and <i>Culex quinquefasciatus</i> larvae in semi-field larvicide trials conducted outdoors in St. Augustine, Trinidad, where 100% of the larvae were dead within 24 h. The yeast was also stably suspended in commercial bait and deployed as an active ingredient in miniature attractive targeted sugar bait (ATSB) station sachets. The yeast ATSB induced high levels of <i>Aedes</i> and <i>Culex</i> mosquito morbidity in semi-field trials conducted in Trinidad, West Indies, as well as in Bangkok, Thailand, in which the consumption of the yeast resulted in adult female mosquito death within 48 h, faster than what was observed in laboratory trials. These findings support the pursuit of large-scale field trials to further evaluate the Sh.463_56.10R insecticide, a member of a promising new class of species-specific RNAi insecticides that could help combat insecticide resistance and support effective mosquito control programs worldwide.https://www.mdpi.com/2075-4450/14/12/950<i>Aedes aegypti</i><i>Aedes albopictus</i><i>Culex quinquefasciatus</i><i>Saccharomyces cerevisiae</i>larvicideinsecticide |
| spellingShingle | Akilah T. M. Stewart Keshava Mysore Teresia M. Njoroge Nikhella Winter Rachel Shui Feng Satish Singh Lester D. James Preeraya Singkhaimuk Longhua Sun Azad Mohammed James D. Oxley Craig Duckham Alongkot Ponlawat David W. Severson Molly Duman-Scheel Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes Insects <i>Aedes aegypti</i> <i>Aedes albopictus</i> <i>Culex quinquefasciatus</i> <i>Saccharomyces cerevisiae</i> larvicide insecticide |
| title | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes |
| title_full | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes |
| title_fullStr | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes |
| title_full_unstemmed | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes |
| title_short | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on <i>Aedes</i> and <i>Culex</i> Mosquitoes |
| title_sort | demonstration of rnai yeast insecticide activity in semi field larvicide and attractive targeted sugar bait trials conducted on i aedes i and i culex i mosquitoes |
| topic | <i>Aedes aegypti</i> <i>Aedes albopictus</i> <i>Culex quinquefasciatus</i> <i>Saccharomyces cerevisiae</i> larvicide insecticide |
| url | https://www.mdpi.com/2075-4450/14/12/950 |
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