Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour
Abstract Background Dietary and wholesome antioxidant rutin is considered advantageous due to its potential protective role for numerous diseases related to oxidative stress, high safety, cost-effectiveness, and extensive biological effects. The present study accounts for an expeditious method for t...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-09-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-022-00297-x |
| _version_ | 1811265225406545920 |
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| author | Kinjal Kubavat Pooja Trivedi Hafsa Ansari Anita Kongor Manthan Panchal Vinod Jain Gaurang Sindhav |
| author_facet | Kinjal Kubavat Pooja Trivedi Hafsa Ansari Anita Kongor Manthan Panchal Vinod Jain Gaurang Sindhav |
| author_sort | Kinjal Kubavat |
| collection | DOAJ |
| description | Abstract Background Dietary and wholesome antioxidant rutin is considered advantageous due to its potential protective role for numerous diseases related to oxidative stress, high safety, cost-effectiveness, and extensive biological effects. The present study accounts for an expeditious method for the synthesis of silver nanoparticles (AgNPs) using rutin. Results The presence of AgNPs was affirmed by UV–visible spectroscopy at 425 nm, and FESEM and zeta sizer analysis revealed the average size of the AgNPs 80–85 nm and 160 d.nm, respectively. Zeta potential measurements (− 30.3 mV) showed that the AgNPs have reasonably good stability. Element mapping analysis of the AgNPs was confirmed by XRD and AFM, while FTIR spectra of the AgNPs showed the existence of functional groups. In the DPPH assay, highest radical scavenging activity of AgNPs, 86.95 ± 01.60%, was confirmed. The interaction of AgNPs with CT-DNA and HS-DNA was studied spectrophotometrically, and the data display a shift in the respective spectra. Furthermore, interaction with pBR322 DNA, λ DNA, CT-DNA, and HS-DNA was deliberated by a nicking assay that shows the physicochemical properties of AgNPs. Antibacterial activity was evaluated by the standard well-diffusion method against Escherichia coli and Staphylococcus aureus, and cytotoxicity was assessed against human WBCs by MTT assay. Conclusion As per this appraisal, it can be concluded that it is a cost-effective, simple, and eco-friendly tactic and such NPs are beneficial to improve therapeutics since the antioxidant, DNA interaction, antibacterial, and cytotoxic exploits offer a new horizon of euthenics. |
| first_indexed | 2024-04-12T20:18:44Z |
| format | Article |
| id | doaj.art-eb50834d5ee04179ac30cf7a2a026895 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-04-12T20:18:44Z |
| publishDate | 2022-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-eb50834d5ee04179ac30cf7a2a0268952022-12-22T03:18:03ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432022-09-0111111410.1186/s43088-022-00297-xGreen synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contourKinjal Kubavat0Pooja Trivedi1Hafsa Ansari2Anita Kongor3Manthan Panchal4Vinod Jain5Gaurang Sindhav6Department of Zoology, BMT, HGC and WBC, University School of Sciences, Gujarat UniversityDepartment of Zoology, BMT, HGC and WBC, University School of Sciences, Gujarat UniversityDepartment of Zoology, BMT, HGC and WBC, University School of Sciences, Gujarat UniversityDepartment of Chemistry, University School of Sciences, Gujarat UniversityDepartment of Chemistry, University School of Sciences, Gujarat UniversityDepartment of Chemistry, University School of Sciences, Gujarat UniversityDepartment of Zoology, BMT, HGC and WBC, University School of Sciences, Gujarat UniversityAbstract Background Dietary and wholesome antioxidant rutin is considered advantageous due to its potential protective role for numerous diseases related to oxidative stress, high safety, cost-effectiveness, and extensive biological effects. The present study accounts for an expeditious method for the synthesis of silver nanoparticles (AgNPs) using rutin. Results The presence of AgNPs was affirmed by UV–visible spectroscopy at 425 nm, and FESEM and zeta sizer analysis revealed the average size of the AgNPs 80–85 nm and 160 d.nm, respectively. Zeta potential measurements (− 30.3 mV) showed that the AgNPs have reasonably good stability. Element mapping analysis of the AgNPs was confirmed by XRD and AFM, while FTIR spectra of the AgNPs showed the existence of functional groups. In the DPPH assay, highest radical scavenging activity of AgNPs, 86.95 ± 01.60%, was confirmed. The interaction of AgNPs with CT-DNA and HS-DNA was studied spectrophotometrically, and the data display a shift in the respective spectra. Furthermore, interaction with pBR322 DNA, λ DNA, CT-DNA, and HS-DNA was deliberated by a nicking assay that shows the physicochemical properties of AgNPs. Antibacterial activity was evaluated by the standard well-diffusion method against Escherichia coli and Staphylococcus aureus, and cytotoxicity was assessed against human WBCs by MTT assay. Conclusion As per this appraisal, it can be concluded that it is a cost-effective, simple, and eco-friendly tactic and such NPs are beneficial to improve therapeutics since the antioxidant, DNA interaction, antibacterial, and cytotoxic exploits offer a new horizon of euthenics.https://doi.org/10.1186/s43088-022-00297-xRutinSilver nanoparticles (AgNPs)SpectroscopyDNAAntibacterial activityCytotoxicity |
| spellingShingle | Kinjal Kubavat Pooja Trivedi Hafsa Ansari Anita Kongor Manthan Panchal Vinod Jain Gaurang Sindhav Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour Beni-Suef University Journal of Basic and Applied Sciences Rutin Silver nanoparticles (AgNPs) Spectroscopy DNA Antibacterial activity Cytotoxicity |
| title | Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| title_full | Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| title_fullStr | Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| title_full_unstemmed | Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| title_short | Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| title_sort | green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour |
| topic | Rutin Silver nanoparticles (AgNPs) Spectroscopy DNA Antibacterial activity Cytotoxicity |
| url | https://doi.org/10.1186/s43088-022-00297-x |
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