Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet
The effects of rumen buffer agents on ruminal fermentation parameters and bacterial community composition were determined using in vitro and in vivo experiments in three rumen-cannulated, high-concentrate fed Holstein Friesian dairy cows. Experiment 1 in vitro treatments included bentonite, calcium...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/11/6/554 |
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| author | Sonny C. Ramos Chang-Dae Jeong Lovelia L. Mamuad Seon-Ho Kim A-Rang Son Michelle A. Miguel Mahfuzul Islam Yong-Il Cho Sang-Suk Lee |
| author_facet | Sonny C. Ramos Chang-Dae Jeong Lovelia L. Mamuad Seon-Ho Kim A-Rang Son Michelle A. Miguel Mahfuzul Islam Yong-Il Cho Sang-Suk Lee |
| author_sort | Sonny C. Ramos |
| collection | DOAJ |
| description | The effects of rumen buffer agents on ruminal fermentation parameters and bacterial community composition were determined using in vitro and in vivo experiments in three rumen-cannulated, high-concentrate fed Holstein Friesian dairy cows. Experiment 1 in vitro treatments included bentonite, calcium carbonate, calcium oxide, sodium bicarbonate, sodium sesquicarbonate, and processed coral, and unbuffered samples served as the control. Experiment 2 in vitro treatments were based on the formulation of various combinations of the buffer agents used in Experiment 1. Combinations were selected for the in vivo study based on their buffering ability. Calcium oxide, sodium bicarbonate, and sodium sesquicarbonate stabilized the ruminal pH and improved in vitro rumen fermentation. The combined buffer agents had a significant effect on pH, buffering capacity, total gas, and total volatile fatty acids. Firmicutes and Bacteroidetes were the dominant phyla in both treatments and the control. <i>Ruminococcus</i> and <i>Prevotella</i> were found to be the dominant genera. <i>Ruminococcus bromii</i> was predominant in the treatment group. <i>Prevotella jejuni</i> was more abundant in the control group compared to the treatment group, in which its abundance was very low. <i>Ruminococcus flavefaciens</i> and <i>Intestinimonas butyriciproducens</i> gradually increased in abundance as cows received treatment. Overall, a high-concentrate diet administered to cows induced adverse changes in ruminal pH; however, buffer supplementation enhanced ruminal fermentation characteristics and altered bacterial community, which could contribute to preventing ruminal acidosis. |
| first_indexed | 2024-03-10T10:20:00Z |
| format | Article |
| id | doaj.art-eb51fa77b0b04d0cb0e2482aadfef57d |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T10:20:00Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-eb51fa77b0b04d0cb0e2482aadfef57d2023-11-22T00:33:45ZengMDPI AGAgriculture2077-04722021-06-0111655410.3390/agriculture11060554Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate DietSonny C. Ramos0Chang-Dae Jeong1Lovelia L. Mamuad2Seon-Ho Kim3A-Rang Son4Michelle A. Miguel5Mahfuzul Islam6Yong-Il Cho7Sang-Suk Lee8Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaAnimal Disease and Diagnostic Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaRuminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, KoreaThe effects of rumen buffer agents on ruminal fermentation parameters and bacterial community composition were determined using in vitro and in vivo experiments in three rumen-cannulated, high-concentrate fed Holstein Friesian dairy cows. Experiment 1 in vitro treatments included bentonite, calcium carbonate, calcium oxide, sodium bicarbonate, sodium sesquicarbonate, and processed coral, and unbuffered samples served as the control. Experiment 2 in vitro treatments were based on the formulation of various combinations of the buffer agents used in Experiment 1. Combinations were selected for the in vivo study based on their buffering ability. Calcium oxide, sodium bicarbonate, and sodium sesquicarbonate stabilized the ruminal pH and improved in vitro rumen fermentation. The combined buffer agents had a significant effect on pH, buffering capacity, total gas, and total volatile fatty acids. Firmicutes and Bacteroidetes were the dominant phyla in both treatments and the control. <i>Ruminococcus</i> and <i>Prevotella</i> were found to be the dominant genera. <i>Ruminococcus bromii</i> was predominant in the treatment group. <i>Prevotella jejuni</i> was more abundant in the control group compared to the treatment group, in which its abundance was very low. <i>Ruminococcus flavefaciens</i> and <i>Intestinimonas butyriciproducens</i> gradually increased in abundance as cows received treatment. Overall, a high-concentrate diet administered to cows induced adverse changes in ruminal pH; however, buffer supplementation enhanced ruminal fermentation characteristics and altered bacterial community, which could contribute to preventing ruminal acidosis.https://www.mdpi.com/2077-0472/11/6/554ruminal fermentationbacteria communityHolstein Friesian cowsbuffer agents |
| spellingShingle | Sonny C. Ramos Chang-Dae Jeong Lovelia L. Mamuad Seon-Ho Kim A-Rang Son Michelle A. Miguel Mahfuzul Islam Yong-Il Cho Sang-Suk Lee Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet Agriculture ruminal fermentation bacteria community Holstein Friesian cows buffer agents |
| title | Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet |
| title_full | Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet |
| title_fullStr | Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet |
| title_full_unstemmed | Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet |
| title_short | Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet |
| title_sort | enhanced ruminal fermentation parameters and altered rumen bacterial community composition by formulated rumen buffer agents fed to dairy cows with a high concentrate diet |
| topic | ruminal fermentation bacteria community Holstein Friesian cows buffer agents |
| url | https://www.mdpi.com/2077-0472/11/6/554 |
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