Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats
Pharmacological inhibition of cyclooxygenase-2 (COX-2) activity ameliorated the severity of non-alcoholic steatohepatitis (NASH) rats. It is not completely understood that the role of COX-2 inhibitor celecoxib on adiponectin receptors (Adipo-R1/R2) expression in different tissues in NASH rats. Sprag...
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Elsevier
2022-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022011604 |
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| author | Guoying Zhu Li Chen Su Liu Ling She Yongnian Ding Changqing Yang Fengshang Zhu |
| author_facet | Guoying Zhu Li Chen Su Liu Ling She Yongnian Ding Changqing Yang Fengshang Zhu |
| author_sort | Guoying Zhu |
| collection | DOAJ |
| description | Pharmacological inhibition of cyclooxygenase-2 (COX-2) activity ameliorated the severity of non-alcoholic steatohepatitis (NASH) rats. It is not completely understood that the role of COX-2 inhibitor celecoxib on adiponectin receptors (Adipo-R1/R2) expression in different tissues in NASH rats. Sprague-Dawley male NASH rats induced by a high-fat diet (HFD) were administrated with or without celecoxib for 8 weeks. Biochemical parameters of liver function, glucose, and lipid metabolism, and the levels of adiponectin, tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2) in the serum or liver were collected according to the standard protocols. The mRNA and protein levels of Adipo-R1, Adipo-R2, and COX-2 in the liver, muscle, and visceral fat were performed by quantitative real-time polymerase chain reaction (q-PCR) and Western blot analysis, respectively. The results showed that celecoxib ameliorated the various clinical indicators and pathological characteristics in the NASH rats, including body weight, liver function, liver index, and redox activities in serum and hepatic samples. The serum concentrations of adiponectin and TNF-α and PGE2 were negatively correlated. As expected, these ameliorative effects of celecoxib were associated with the gene and protein levels up-regulation of Adipo-R1, Adipo-R2 in the liver and visceral fat tissues, and seeming to be compensatory down-regulation expression in muscle tissues (P <0.05). Additionally, COX-2 protein expression was negatively correlated with serum adiponectin levels, protein expression of adiponectin receptors from the liver and visceral fat, conversely, positively correlated with those from the muscle. Our current study demonstrate that celecoxib might effectively alleviate NASH rats in a unique manner closely relevant to redistributing the expression of adiponectin receptors in the liver, visceral fat, and muscle. However, the precise molecular mechanism needs further study. |
| first_indexed | 2024-12-11T16:46:47Z |
| format | Article |
| id | doaj.art-eb5375801986436e8f4bc9c33759fb2a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T16:46:47Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-eb5375801986436e8f4bc9c33759fb2a2022-12-22T00:58:11ZengElsevierHeliyon2405-84402022-07-0187e09872Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in ratsGuoying Zhu0Li Chen1Su Liu2Ling She3Yongnian Ding4Changqing Yang5Fengshang Zhu6Department of Clinical Nutrition, Putuo People's Hospital, School of Medicine, Tongji University, Shanghai, 200060, ChinaDepartment of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, ChinaDepartment of Gastroenterology, Zhabei Central Hospital of Jingan District, Shanghai, 200070, ChinaState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, ChinaState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, ChinaDepartment of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, ChinaState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China; Department of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, 389 Xincun Road, Shanghai, 200065, China; Corresponding author.Pharmacological inhibition of cyclooxygenase-2 (COX-2) activity ameliorated the severity of non-alcoholic steatohepatitis (NASH) rats. It is not completely understood that the role of COX-2 inhibitor celecoxib on adiponectin receptors (Adipo-R1/R2) expression in different tissues in NASH rats. Sprague-Dawley male NASH rats induced by a high-fat diet (HFD) were administrated with or without celecoxib for 8 weeks. Biochemical parameters of liver function, glucose, and lipid metabolism, and the levels of adiponectin, tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2) in the serum or liver were collected according to the standard protocols. The mRNA and protein levels of Adipo-R1, Adipo-R2, and COX-2 in the liver, muscle, and visceral fat were performed by quantitative real-time polymerase chain reaction (q-PCR) and Western blot analysis, respectively. The results showed that celecoxib ameliorated the various clinical indicators and pathological characteristics in the NASH rats, including body weight, liver function, liver index, and redox activities in serum and hepatic samples. The serum concentrations of adiponectin and TNF-α and PGE2 were negatively correlated. As expected, these ameliorative effects of celecoxib were associated with the gene and protein levels up-regulation of Adipo-R1, Adipo-R2 in the liver and visceral fat tissues, and seeming to be compensatory down-regulation expression in muscle tissues (P <0.05). Additionally, COX-2 protein expression was negatively correlated with serum adiponectin levels, protein expression of adiponectin receptors from the liver and visceral fat, conversely, positively correlated with those from the muscle. Our current study demonstrate that celecoxib might effectively alleviate NASH rats in a unique manner closely relevant to redistributing the expression of adiponectin receptors in the liver, visceral fat, and muscle. However, the precise molecular mechanism needs further study.http://www.sciencedirect.com/science/article/pii/S2405844022011604CelecoxibNon-alcoholic steatohepatitisAdiponectinAdiponectin receptor |
| spellingShingle | Guoying Zhu Li Chen Su Liu Ling She Yongnian Ding Changqing Yang Fengshang Zhu Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats Heliyon Celecoxib Non-alcoholic steatohepatitis Adiponectin Adiponectin receptor |
| title | Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| title_full | Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| title_fullStr | Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| title_full_unstemmed | Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| title_short | Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| title_sort | celecoxib mediated attenuation of non alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats |
| topic | Celecoxib Non-alcoholic steatohepatitis Adiponectin Adiponectin receptor |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022011604 |
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