Responses of Two Contrasting Genotypes of Rice to Brown Planthopper
Rice (Oryza sativa L.) and brown planthoppers (BPH) (Nilaparvata lugens Stål) provide an ideal system for studying molecular mechanisms involved in the interactions between plants and phloem-feeding insects. The phenotypic responses and changes in transcript profiles of seedlings representing two ri...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-1-0122 |
| _version_ | 1819081510493356032 |
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| author | Yuanyuan Wang Xiaolan Wang Hongyu Yuan Rongzhi Chen Lili Zhu Ruifeng He Guangcun He |
| author_facet | Yuanyuan Wang Xiaolan Wang Hongyu Yuan Rongzhi Chen Lili Zhu Ruifeng He Guangcun He |
| author_sort | Yuanyuan Wang |
| collection | DOAJ |
| description | Rice (Oryza sativa L.) and brown planthoppers (BPH) (Nilaparvata lugens Stål) provide an ideal system for studying molecular mechanisms involved in the interactions between plants and phloem-feeding insects. The phenotypic responses and changes in transcript profiles of seedlings representing two rice cultivars differing in resistance to the BPH were analyzed. In the BPH-compatible (susceptible) cv. MH63, BPH feeding reduced three examined plant growth parameters (leaf area expansion, height increases, and dry weight increases) and photosynthetic rates of the leaves. In the BPH-incompatible (resistant) cv. B5, BPH feeding caused slight reductions in protein and sucrose contents, but the plants maintained their photosynthetic activity and grew normally. A cDNA microarray containing 1,920 suppression subtractive hybridization clones was used to explore the transcript profiles differences in the two cultivars under control and BPH-feeding conditions. In total, 160 unique genes were detected as being significantly affected by BPH feeding in rice plants, covering a wide range of functional categories, and there were 38 genes that showed the similar transcript pattern in both genotypes. The physiological responses and transcript profiles of plants represented in both genotypes suggested that multiple pathways might be involved in reprogramming of BPH-infested rice plants. The differences in transcript levels between the compatible and incompatible interactions revealed in this study were not only the reaction of resistance and susceptibility but also reflections of different damage rates and genotypic backgrounds of the rice cultivars. |
| first_indexed | 2024-12-21T20:01:55Z |
| format | Article |
| id | doaj.art-eb54a2949ce34f1da0ab5719f116d3a3 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:01:55Z |
| publishDate | 2008-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-eb54a2949ce34f1da0ab5719f116d3a32022-12-21T18:51:58ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-01-0121112213210.1094/MPMI-21-1-0122Responses of Two Contrasting Genotypes of Rice to Brown PlanthopperYuanyuan WangXiaolan WangHongyu YuanRongzhi ChenLili ZhuRuifeng HeGuangcun HeRice (Oryza sativa L.) and brown planthoppers (BPH) (Nilaparvata lugens Stål) provide an ideal system for studying molecular mechanisms involved in the interactions between plants and phloem-feeding insects. The phenotypic responses and changes in transcript profiles of seedlings representing two rice cultivars differing in resistance to the BPH were analyzed. In the BPH-compatible (susceptible) cv. MH63, BPH feeding reduced three examined plant growth parameters (leaf area expansion, height increases, and dry weight increases) and photosynthetic rates of the leaves. In the BPH-incompatible (resistant) cv. B5, BPH feeding caused slight reductions in protein and sucrose contents, but the plants maintained their photosynthetic activity and grew normally. A cDNA microarray containing 1,920 suppression subtractive hybridization clones was used to explore the transcript profiles differences in the two cultivars under control and BPH-feeding conditions. In total, 160 unique genes were detected as being significantly affected by BPH feeding in rice plants, covering a wide range of functional categories, and there were 38 genes that showed the similar transcript pattern in both genotypes. The physiological responses and transcript profiles of plants represented in both genotypes suggested that multiple pathways might be involved in reprogramming of BPH-infested rice plants. The differences in transcript levels between the compatible and incompatible interactions revealed in this study were not only the reaction of resistance and susceptibility but also reflections of different damage rates and genotypic backgrounds of the rice cultivars.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-1-0122BPH-responsive geneplant–herbivore interactions |
| spellingShingle | Yuanyuan Wang Xiaolan Wang Hongyu Yuan Rongzhi Chen Lili Zhu Ruifeng He Guangcun He Responses of Two Contrasting Genotypes of Rice to Brown Planthopper Molecular Plant-Microbe Interactions BPH-responsive gene plant–herbivore interactions |
| title | Responses of Two Contrasting Genotypes of Rice to Brown Planthopper |
| title_full | Responses of Two Contrasting Genotypes of Rice to Brown Planthopper |
| title_fullStr | Responses of Two Contrasting Genotypes of Rice to Brown Planthopper |
| title_full_unstemmed | Responses of Two Contrasting Genotypes of Rice to Brown Planthopper |
| title_short | Responses of Two Contrasting Genotypes of Rice to Brown Planthopper |
| title_sort | responses of two contrasting genotypes of rice to brown planthopper |
| topic | BPH-responsive gene plant–herbivore interactions |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-1-0122 |
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