The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract
<i>Merremia umbellata</i> Hallier f. (MU) has been used as an anti-inflammatory agent to treat burns and scales. However, the potential anti-inflammatory mechanisms of action of this plant have not been elucidated. This study aimed to assess the antioxidant and anti-inflammatory effects...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/12/2037 |
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| author | Sunny Chung Lee Jongmin Ahn Jina Kim Joo-Yeon Lee Juhae Kim Md. Salah Uddin Sang Woo Lee Choon Young Kim |
| author_facet | Sunny Chung Lee Jongmin Ahn Jina Kim Joo-Yeon Lee Juhae Kim Md. Salah Uddin Sang Woo Lee Choon Young Kim |
| author_sort | Sunny Chung Lee |
| collection | DOAJ |
| description | <i>Merremia umbellata</i> Hallier f. (MU) has been used as an anti-inflammatory agent to treat burns and scales. However, the potential anti-inflammatory mechanisms of action of this plant have not been elucidated. This study aimed to assess the antioxidant and anti-inflammatory effects of the leaf and shoot of MU grown in Bangladesh. The MU extract exhibited antioxidant activities as demonstrated by DPPH and ABTS free-radical-scavenging activities and the total polyphenol and total flavonoid contents. MU extract significantly reduced the lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW264.7 macrophage. Accordingly, the gene levels of inducible NO synthase and cyclooxygenase-2 were suppressed. The MU extract alleviated the LPS-induced expression of TLR4, NF-κB, and inflammatory cytokines (<i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i>). The constituents of a MU extract were tentatively identified using UHPLC-PDA-QTOF/MS techniques. The main compounds were identified as 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, quercitrin, and 4,5-dicaffeoylquinic acid. Molecular docking analysis revealed that these compounds interact with TLR4 protein, with quercitrin showing the highest binding affinity among them. Overall, our findings demonstrate the antioxidant and in vitro anti-inflammatory activities of MU and its potential compounds to target the TLR4-NF-κB signaling pathway. These findings are potentially used to further explore promising natural food ingredients that are effective in regulating inflammation. |
| first_indexed | 2024-03-08T21:03:03Z |
| format | Article |
| id | doaj.art-eb585d12703c420eb91f1183366fcb10 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T21:03:03Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-eb585d12703c420eb91f1183366fcb102023-12-22T13:48:18ZengMDPI AGAntioxidants2076-39212023-11-011212203710.3390/antiox12122037The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> ExtractSunny Chung Lee0Jongmin Ahn1Jina Kim2Joo-Yeon Lee3Juhae Kim4Md. Salah Uddin5Sang Woo Lee6Choon Young Kim7Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095-1735, USANatural Product Central Bank & Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Chungbuk, Republic of KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaResearch Institute of Human Ecology, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaEthnobotanical Database of Bangladesh, Tejgaon, Dhaka 1208, BangladeshInternational Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of Korea<i>Merremia umbellata</i> Hallier f. (MU) has been used as an anti-inflammatory agent to treat burns and scales. However, the potential anti-inflammatory mechanisms of action of this plant have not been elucidated. This study aimed to assess the antioxidant and anti-inflammatory effects of the leaf and shoot of MU grown in Bangladesh. The MU extract exhibited antioxidant activities as demonstrated by DPPH and ABTS free-radical-scavenging activities and the total polyphenol and total flavonoid contents. MU extract significantly reduced the lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW264.7 macrophage. Accordingly, the gene levels of inducible NO synthase and cyclooxygenase-2 were suppressed. The MU extract alleviated the LPS-induced expression of TLR4, NF-κB, and inflammatory cytokines (<i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i>). The constituents of a MU extract were tentatively identified using UHPLC-PDA-QTOF/MS techniques. The main compounds were identified as 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, quercitrin, and 4,5-dicaffeoylquinic acid. Molecular docking analysis revealed that these compounds interact with TLR4 protein, with quercitrin showing the highest binding affinity among them. Overall, our findings demonstrate the antioxidant and in vitro anti-inflammatory activities of MU and its potential compounds to target the TLR4-NF-κB signaling pathway. These findings are potentially used to further explore promising natural food ingredients that are effective in regulating inflammation.https://www.mdpi.com/2076-3921/12/12/2037<i>Merremia umbellata</i> Hallier f.antioxidant activityanti-inflammatory activityUHPLC-PDA-QTOFTLR4/NF-κB pathway |
| spellingShingle | Sunny Chung Lee Jongmin Ahn Jina Kim Joo-Yeon Lee Juhae Kim Md. Salah Uddin Sang Woo Lee Choon Young Kim The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract Antioxidants <i>Merremia umbellata</i> Hallier f. antioxidant activity anti-inflammatory activity UHPLC-PDA-QTOF TLR4/NF-κB pathway |
| title | The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract |
| title_full | The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract |
| title_fullStr | The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract |
| title_full_unstemmed | The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract |
| title_short | The Antioxidant and Anti-Inflammatory Properties of <i>Merremia umbellata</i> Extract |
| title_sort | antioxidant and anti inflammatory properties of i merremia umbellata i extract |
| topic | <i>Merremia umbellata</i> Hallier f. antioxidant activity anti-inflammatory activity UHPLC-PDA-QTOF TLR4/NF-κB pathway |
| url | https://www.mdpi.com/2076-3921/12/12/2037 |
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