Human gut microbiota changes reveal the progression of glucose intolerance.
To explore the relationship of gut microbiota with the development of type 2 diabetes (T2DM), we analyzed 121 subjects who were divided into 3 groups based on their glucose intolerance status: normal glucose tolerance (NGT; n = 44), prediabetes (Pre-DM; n = 64), or newly diagnosed T2DM (n = 13). Gut...
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| Format: | Article |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3754967?pdf=render |
| _version_ | 1819265616662495232 |
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| author | Xiuying Zhang Dongqian Shen Zhiwei Fang Zhuye Jie Xinmin Qiu Chunfang Zhang Yingli Chen Linong Ji |
| author_facet | Xiuying Zhang Dongqian Shen Zhiwei Fang Zhuye Jie Xinmin Qiu Chunfang Zhang Yingli Chen Linong Ji |
| author_sort | Xiuying Zhang |
| collection | DOAJ |
| description | To explore the relationship of gut microbiota with the development of type 2 diabetes (T2DM), we analyzed 121 subjects who were divided into 3 groups based on their glucose intolerance status: normal glucose tolerance (NGT; n = 44), prediabetes (Pre-DM; n = 64), or newly diagnosed T2DM (n = 13). Gut microbiota characterizations were determined with 16S rDNA-based high-throughput sequencing. T2DM-related dysbiosis was observed, including the separation of microbial communities and a change of alpha diversity between the different glucose intolerance statuses. To assess the correlation between metabolic parameters and microbiota diversity, clinical characteristics were also measured and a significant association between metabolic parameters (FPG, CRP) and gut microbiota was found. In addition, a total of 28 operational taxonomic units (OTUs) were found to be related to T2DM status by the Kruskal-Wallis H test, most of which were enriched in the T2DM group. Butyrate-producing bacteria (e.g. Akkermansia muciniphila ATCCBAA-835, and Faecalibacterium prausnitzii L2-6) had a higher abundance in the NGT group than in the pre-DM group. At genus level, the abundance of Bacteroides in the T2DM group was only half that of the NGT and Pre-DM groups. Previously reported T2DM-related markers were also compared with the data in this study, and some inconsistencies were noted. We found that Verrucomicrobiae may be a potential marker of T2DM as it had a significantly lower abundance in both the pre-DM and T2DM groups. In conclusion, this research provides further evidence of the structural modulation of gut microbiota in the pathogenesis of diabetes. |
| first_indexed | 2024-12-23T20:48:13Z |
| format | Article |
| id | doaj.art-eb58d9ac56aa462fb32944fc146195c4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T20:48:13Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-eb58d9ac56aa462fb32944fc146195c42022-12-21T17:31:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7110810.1371/journal.pone.0071108Human gut microbiota changes reveal the progression of glucose intolerance.Xiuying ZhangDongqian ShenZhiwei FangZhuye JieXinmin QiuChunfang ZhangYingli ChenLinong JiTo explore the relationship of gut microbiota with the development of type 2 diabetes (T2DM), we analyzed 121 subjects who were divided into 3 groups based on their glucose intolerance status: normal glucose tolerance (NGT; n = 44), prediabetes (Pre-DM; n = 64), or newly diagnosed T2DM (n = 13). Gut microbiota characterizations were determined with 16S rDNA-based high-throughput sequencing. T2DM-related dysbiosis was observed, including the separation of microbial communities and a change of alpha diversity between the different glucose intolerance statuses. To assess the correlation between metabolic parameters and microbiota diversity, clinical characteristics were also measured and a significant association between metabolic parameters (FPG, CRP) and gut microbiota was found. In addition, a total of 28 operational taxonomic units (OTUs) were found to be related to T2DM status by the Kruskal-Wallis H test, most of which were enriched in the T2DM group. Butyrate-producing bacteria (e.g. Akkermansia muciniphila ATCCBAA-835, and Faecalibacterium prausnitzii L2-6) had a higher abundance in the NGT group than in the pre-DM group. At genus level, the abundance of Bacteroides in the T2DM group was only half that of the NGT and Pre-DM groups. Previously reported T2DM-related markers were also compared with the data in this study, and some inconsistencies were noted. We found that Verrucomicrobiae may be a potential marker of T2DM as it had a significantly lower abundance in both the pre-DM and T2DM groups. In conclusion, this research provides further evidence of the structural modulation of gut microbiota in the pathogenesis of diabetes.http://europepmc.org/articles/PMC3754967?pdf=render |
| spellingShingle | Xiuying Zhang Dongqian Shen Zhiwei Fang Zhuye Jie Xinmin Qiu Chunfang Zhang Yingli Chen Linong Ji Human gut microbiota changes reveal the progression of glucose intolerance. PLoS ONE |
| title | Human gut microbiota changes reveal the progression of glucose intolerance. |
| title_full | Human gut microbiota changes reveal the progression of glucose intolerance. |
| title_fullStr | Human gut microbiota changes reveal the progression of glucose intolerance. |
| title_full_unstemmed | Human gut microbiota changes reveal the progression of glucose intolerance. |
| title_short | Human gut microbiota changes reveal the progression of glucose intolerance. |
| title_sort | human gut microbiota changes reveal the progression of glucose intolerance |
| url | http://europepmc.org/articles/PMC3754967?pdf=render |
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