Reverse engineering the yeast RNR1 transcriptional control system.
Transcription is controlled by multi-protein complexes binding to short non-coding regions of genomic DNA. These complexes interact combinatorially. A major goal of modern biology is to provide simple models that predict this complex behavior. The yeast gene RNR1 is transcribed periodically during t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2982837?pdf=render |
| _version_ | 1818841290258776064 |
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| author | Grace Mao James P Brody |
| author_facet | Grace Mao James P Brody |
| author_sort | Grace Mao |
| collection | DOAJ |
| description | Transcription is controlled by multi-protein complexes binding to short non-coding regions of genomic DNA. These complexes interact combinatorially. A major goal of modern biology is to provide simple models that predict this complex behavior. The yeast gene RNR1 is transcribed periodically during the cell cycle. Here, we present a pilot study to demonstrate a new method of deciphering the logic behind transcriptional regulation. We took regular samples from cell cycle synchronized cultures of Saccharomyces cerevisiae and extracted nuclear protein. We tested these samples to measure the amount of protein that bound to seven different 16 base pair sequences of DNA that have been previously identified as protein binding locations in the promoter of the RNR1 gene. These tests were performed using surface plasmon resonance. We found that the surface plasmon resonance signals showed significant variation throughout the cell cycle. We correlated the protein binding data with previously published mRNA expression data and interpreted this to show that transcription requires protein bound to a particular site and either five different sites or one additional sites. We conclude that this demonstrates the feasibility of this approach to decipher the combinatorial logic of transcription. |
| first_indexed | 2024-12-19T04:23:44Z |
| format | Article |
| id | doaj.art-eb597d84cc5949a298461dd8caf68d26 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:23:44Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eb597d84cc5949a298461dd8caf68d262022-12-21T20:36:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01511e1389510.1371/journal.pone.0013895Reverse engineering the yeast RNR1 transcriptional control system.Grace MaoJames P BrodyTranscription is controlled by multi-protein complexes binding to short non-coding regions of genomic DNA. These complexes interact combinatorially. A major goal of modern biology is to provide simple models that predict this complex behavior. The yeast gene RNR1 is transcribed periodically during the cell cycle. Here, we present a pilot study to demonstrate a new method of deciphering the logic behind transcriptional regulation. We took regular samples from cell cycle synchronized cultures of Saccharomyces cerevisiae and extracted nuclear protein. We tested these samples to measure the amount of protein that bound to seven different 16 base pair sequences of DNA that have been previously identified as protein binding locations in the promoter of the RNR1 gene. These tests were performed using surface plasmon resonance. We found that the surface plasmon resonance signals showed significant variation throughout the cell cycle. We correlated the protein binding data with previously published mRNA expression data and interpreted this to show that transcription requires protein bound to a particular site and either five different sites or one additional sites. We conclude that this demonstrates the feasibility of this approach to decipher the combinatorial logic of transcription.http://europepmc.org/articles/PMC2982837?pdf=render |
| spellingShingle | Grace Mao James P Brody Reverse engineering the yeast RNR1 transcriptional control system. PLoS ONE |
| title | Reverse engineering the yeast RNR1 transcriptional control system. |
| title_full | Reverse engineering the yeast RNR1 transcriptional control system. |
| title_fullStr | Reverse engineering the yeast RNR1 transcriptional control system. |
| title_full_unstemmed | Reverse engineering the yeast RNR1 transcriptional control system. |
| title_short | Reverse engineering the yeast RNR1 transcriptional control system. |
| title_sort | reverse engineering the yeast rnr1 transcriptional control system |
| url | http://europepmc.org/articles/PMC2982837?pdf=render |
| work_keys_str_mv | AT gracemao reverseengineeringtheyeastrnr1transcriptionalcontrolsystem AT jamespbrody reverseengineeringtheyeastrnr1transcriptionalcontrolsystem |