Effect of Hedera Helix on Lung Histopathology in Chronic Asthma
Hedera helix is widely used to treat bronchial asthma for many years. However, effects of this herb on lung histopathology is still far from clear. We aimed to determine the effect of oral administration of Hedera helix on lung histopathology in a murine model of chronic asthma. BALB/c mice were ...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-12-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/546 |
| _version_ | 1818127174012502016 |
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| author | Arzu Babayigit Hocaoglu Ozkan Karaman Duygu Olmez Erge Guven Erbil Osman Yilmaz Bijen Kivcak H Alper Bagriyanik Nevin Uzuner |
| author_facet | Arzu Babayigit Hocaoglu Ozkan Karaman Duygu Olmez Erge Guven Erbil Osman Yilmaz Bijen Kivcak H Alper Bagriyanik Nevin Uzuner |
| author_sort | Arzu Babayigit Hocaoglu |
| collection | DOAJ |
| description | Hedera helix is widely used to treat bronchial asthma for many years. However, effects of this herb on lung histopathology is still far from clear. We aimed to determine the effect of oral administration of Hedera helix on lung histopathology in a murine model of chronic asthma.
BALB/c mice were divided into four groups; I (Placebo), II (Hedera helix), III (Dexamethasone) and IV (Control). All mice except controls were sensitized and challenged with ovalbumin. Then, mice in group I received saline, group II 100 mg/kg Hedera helix and group III 1 mg/kg dexamethasone via orogastic gavage once daily for one week. Airway histopathology was evaluated by using light and electron microscopy in all groups.
Goblet cell numbers and thicknesses of basement membrane were found significantly lower in group II, but there was no statistically significant difference in terms of number of mast cells, thicknesses of epithelium and subepithelial smooth muscle layers between group I and II. When Hedera helix and dexamethasone groups were compared with each other, thickness of epithelium, subepithelial muscle layers, number of mast cells and goblet cells of group III were significantly ameliorated when compared with the group II.
Although Hedera helix administration reduced only goblet cell counts and the thicknesses of basement membrane in the asthmatic airways, dexamethasone ameliorated all histopathologic parameters except thickness of basement membrane better than Hedera helix. |
| first_indexed | 2024-12-11T07:13:09Z |
| format | Article |
| id | doaj.art-eb5b489d1f86440da1d589e790c29e3b |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-11T07:13:09Z |
| publishDate | 2012-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-eb5b489d1f86440da1d589e790c29e3b2022-12-22T01:16:18ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492012-12-01114511Effect of Hedera Helix on Lung Histopathology in Chronic AsthmaArzu Babayigit Hocaoglu0Ozkan Karaman1Duygu Olmez Erge2Guven Erbil3Osman Yilmaz4Bijen Kivcak5H Alper Bagriyanik6Nevin Uzuner7Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, TurkeyDepartment of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, TurkeyDepartment of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, TurkeyDepartment of Histology and Embriology, Dokuz Eylul University Hospital, Balcova, Izmir, TurkeyDepartment of Multidisciplinary Animal Laboratory Balcova, Dokuz Eylul University Hospital, Izmir, TurkeyDepartment of Pharmacognosy, Ege University, Faculty of Pharmacy, Bornova, Izmir, TurkeyDepartment of Histology and Embriology, Dokuz Eylul University Hospital, Balcova, Izmir, TurkeyDepartment of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, TurkeyHedera helix is widely used to treat bronchial asthma for many years. However, effects of this herb on lung histopathology is still far from clear. We aimed to determine the effect of oral administration of Hedera helix on lung histopathology in a murine model of chronic asthma. BALB/c mice were divided into four groups; I (Placebo), II (Hedera helix), III (Dexamethasone) and IV (Control). All mice except controls were sensitized and challenged with ovalbumin. Then, mice in group I received saline, group II 100 mg/kg Hedera helix and group III 1 mg/kg dexamethasone via orogastic gavage once daily for one week. Airway histopathology was evaluated by using light and electron microscopy in all groups. Goblet cell numbers and thicknesses of basement membrane were found significantly lower in group II, but there was no statistically significant difference in terms of number of mast cells, thicknesses of epithelium and subepithelial smooth muscle layers between group I and II. When Hedera helix and dexamethasone groups were compared with each other, thickness of epithelium, subepithelial muscle layers, number of mast cells and goblet cells of group III were significantly ameliorated when compared with the group II. Although Hedera helix administration reduced only goblet cell counts and the thicknesses of basement membrane in the asthmatic airways, dexamethasone ameliorated all histopathologic parameters except thickness of basement membrane better than Hedera helix.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/546Airway remodelingAsthmaBALB c miceHedera helixMicroscopy electron |
| spellingShingle | Arzu Babayigit Hocaoglu Ozkan Karaman Duygu Olmez Erge Guven Erbil Osman Yilmaz Bijen Kivcak H Alper Bagriyanik Nevin Uzuner Effect of Hedera Helix on Lung Histopathology in Chronic Asthma Iranian Journal of Allergy, Asthma and Immunology Airway remodeling Asthma BALB c mice Hedera helix Microscopy electron |
| title | Effect of Hedera Helix on Lung Histopathology in Chronic Asthma |
| title_full | Effect of Hedera Helix on Lung Histopathology in Chronic Asthma |
| title_fullStr | Effect of Hedera Helix on Lung Histopathology in Chronic Asthma |
| title_full_unstemmed | Effect of Hedera Helix on Lung Histopathology in Chronic Asthma |
| title_short | Effect of Hedera Helix on Lung Histopathology in Chronic Asthma |
| title_sort | effect of hedera helix on lung histopathology in chronic asthma |
| topic | Airway remodeling Asthma BALB c mice Hedera helix Microscopy electron |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/546 |
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