Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix
Ophiopogonis Radix (OR) is a traditional Chinese medicine. In recent years, in order to achieve the purpose of drying, bleaching, sterilizing and being antiseptic, improving appearance, and easy storage, people often use sulfur fumigation for its processing. However, changes in the chemical composit...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/3/702 |
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| author | Yanan Li Pingping Dong Zhanpeng Shang Long Dai Shaoping Wang Jiayu Zhang |
| author_facet | Yanan Li Pingping Dong Zhanpeng Shang Long Dai Shaoping Wang Jiayu Zhang |
| author_sort | Yanan Li |
| collection | DOAJ |
| description | Ophiopogonis Radix (OR) is a traditional Chinese medicine. In recent years, in order to achieve the purpose of drying, bleaching, sterilizing and being antiseptic, improving appearance, and easy storage, people often use sulfur fumigation for its processing. However, changes in the chemical composition of medicinal herbs caused by sulfur fumigation can lead to the transformation and loss of potent substances. Therefore, the development of methods to rapidly reveal the chemical transformation of medicinal herbs induced by sulfur fumigation can guarantee the safe clinical use of medicines. In this study, a combined full scan-parent ions list-dynamic exclusion acquisition-diagnostic product ions analysis strategy based on UHPLC-LTQ-Orbitrap MS was proposed for the analysis of steroidal saponins and their transformed components in sulfur-fumigated Ophiopogonis Radix (SF-OR). Based on precise mass measurements, chromatographic behavior, neutral loss ions, and diagnostic product ions, 286 constituents were screened and identified from SF-OR, including 191 steroidal saponins and 95 sulfur-containing derivatives (sulfates or sulfites). The results indicated that the established strategy was a valuable and effective analytical tool for comprehensively characterizing the material basis of SF-OR, and also provided a basis for potential chemical changes in other sulfur-fumigated herbs. |
| first_indexed | 2024-03-08T03:52:14Z |
| format | Article |
| id | doaj.art-eb5ccaea2cb845b7b0ffd27fbe04eb8a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T03:52:14Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-eb5ccaea2cb845b7b0ffd27fbe04eb8a2024-02-09T15:19:08ZengMDPI AGMolecules1420-30492024-02-0129370210.3390/molecules29030702Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis RadixYanan Li0Pingping Dong1Zhanpeng Shang2Long Dai3Shaoping Wang4Jiayu Zhang5School of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, ChinaState Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Macao SAR 999078, ChinaSchool of Pharmacy, Beijing University of Chinese Medicine, Beijing 100191, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaSchool of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, ChinaSchool of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, ChinaOphiopogonis Radix (OR) is a traditional Chinese medicine. In recent years, in order to achieve the purpose of drying, bleaching, sterilizing and being antiseptic, improving appearance, and easy storage, people often use sulfur fumigation for its processing. However, changes in the chemical composition of medicinal herbs caused by sulfur fumigation can lead to the transformation and loss of potent substances. Therefore, the development of methods to rapidly reveal the chemical transformation of medicinal herbs induced by sulfur fumigation can guarantee the safe clinical use of medicines. In this study, a combined full scan-parent ions list-dynamic exclusion acquisition-diagnostic product ions analysis strategy based on UHPLC-LTQ-Orbitrap MS was proposed for the analysis of steroidal saponins and their transformed components in sulfur-fumigated Ophiopogonis Radix (SF-OR). Based on precise mass measurements, chromatographic behavior, neutral loss ions, and diagnostic product ions, 286 constituents were screened and identified from SF-OR, including 191 steroidal saponins and 95 sulfur-containing derivatives (sulfates or sulfites). The results indicated that the established strategy was a valuable and effective analytical tool for comprehensively characterizing the material basis of SF-OR, and also provided a basis for potential chemical changes in other sulfur-fumigated herbs.https://www.mdpi.com/1420-3049/29/3/702Ophiopogonis Radixsulphur fumigationUHPLC-LTQ-Orbitrapsteroidal saponinspaired-diagnostic product ionneutral loss filter |
| spellingShingle | Yanan Li Pingping Dong Zhanpeng Shang Long Dai Shaoping Wang Jiayu Zhang Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix Molecules Ophiopogonis Radix sulphur fumigation UHPLC-LTQ-Orbitrap steroidal saponins paired-diagnostic product ion neutral loss filter |
| title | Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix |
| title_full | Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix |
| title_fullStr | Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix |
| title_full_unstemmed | Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix |
| title_short | Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS—A Case Study on Steroidal Saponins in Ophiopogonis Radix |
| title_sort | unveiling the chemical composition of sulfur fumigated herbs a triple synthesis approach using uhplc ltq orbitrap ms a case study on steroidal saponins in ophiopogonis radix |
| topic | Ophiopogonis Radix sulphur fumigation UHPLC-LTQ-Orbitrap steroidal saponins paired-diagnostic product ion neutral loss filter |
| url | https://www.mdpi.com/1420-3049/29/3/702 |
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