CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Abstract CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an aut...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-06-01
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| Series: | Genome Biology |
| Online Access: | http://link.springer.com/article/10.1186/s13059-017-1237-8 |