Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro
Induced pluripotent stem cell secretome (iPSC-CM) mitigate organ injury and help in repair. Macrophages play a critical role in tissue repair and regeneration and can be directed to promote tissue repair by iPSC-CM, although the exact mechanisms are not known. In the current investigative study, we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/958 |
| _version_ | 1797409777143250944 |
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| author | Luca Tamò Kleanthis Fytianos Fabienne Caldana Cedric Simillion Anis Feki Izabela Nita Manfred Heller Thomas Geiser Amiq Gazdhar |
| author_facet | Luca Tamò Kleanthis Fytianos Fabienne Caldana Cedric Simillion Anis Feki Izabela Nita Manfred Heller Thomas Geiser Amiq Gazdhar |
| author_sort | Luca Tamò |
| collection | DOAJ |
| description | Induced pluripotent stem cell secretome (iPSC-CM) mitigate organ injury and help in repair. Macrophages play a critical role in tissue repair and regeneration and can be directed to promote tissue repair by iPSC-CM, although the exact mechanisms are not known. In the current investigative study, we evaluated the possible mechanism by which iPSC-CM regulates the phenotype and secretory pattern of macrophages in vitro. Macrophages were obtained from human peripheral blood mononuclear cells and differentiated to various subpopulations and treated with either iPSC-CM or control media in vitro. Macrophage phenotype was assessed by flow cytometry, gene expression changes by qRT PCR and secretory pattern by multiplex protein analysis. The protein and gene interaction network revealed the involvement of Amyloid precursor protein (APP) and ELAV-like protein 1 (ELAVL-1) both present in the iPSC-CM to play an important role in regulating the macrophage phenotype and their secretory pattern. This exploratory study reveals, in part, the possible mechanism and identifies two potential targets by which iPSC-CM regulate macrophages and help in repair and regeneration. |
| first_indexed | 2024-03-09T04:19:30Z |
| format | Article |
| id | doaj.art-eb5d93a0ecd64ea59276c6ea13105ba8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:19:30Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eb5d93a0ecd64ea59276c6ea13105ba82023-12-03T13:49:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122295810.3390/ijms22020958Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In VitroLuca Tamò0Kleanthis Fytianos1Fabienne Caldana2Cedric Simillion3Anis Feki4Izabela Nita5Manfred Heller6Thomas Geiser7Amiq Gazdhar8Department of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandDepartment of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandDepartment of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandDepartment of BioMedical Research, University of Bern, 3008 Bern, SwitzerlandDepartment of Gynecology and Obstetrics, Cantonal Hospital Fribourg, 1702 Fribourg, SwitzerlandDepartment of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandDepartment of BioMedical Research, University of Bern, 3008 Bern, SwitzerlandDepartment of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandDepartment of Pulmonary Medicine, University Hospital Bern, 3008 Bern, SwitzerlandInduced pluripotent stem cell secretome (iPSC-CM) mitigate organ injury and help in repair. Macrophages play a critical role in tissue repair and regeneration and can be directed to promote tissue repair by iPSC-CM, although the exact mechanisms are not known. In the current investigative study, we evaluated the possible mechanism by which iPSC-CM regulates the phenotype and secretory pattern of macrophages in vitro. Macrophages were obtained from human peripheral blood mononuclear cells and differentiated to various subpopulations and treated with either iPSC-CM or control media in vitro. Macrophage phenotype was assessed by flow cytometry, gene expression changes by qRT PCR and secretory pattern by multiplex protein analysis. The protein and gene interaction network revealed the involvement of Amyloid precursor protein (APP) and ELAV-like protein 1 (ELAVL-1) both present in the iPSC-CM to play an important role in regulating the macrophage phenotype and their secretory pattern. This exploratory study reveals, in part, the possible mechanism and identifies two potential targets by which iPSC-CM regulate macrophages and help in repair and regeneration.https://www.mdpi.com/1422-0067/22/2/958lung fibrosismacrophagesinduced pluripotent stem cellsstem cells secretomelung repair and regeneration |
| spellingShingle | Luca Tamò Kleanthis Fytianos Fabienne Caldana Cedric Simillion Anis Feki Izabela Nita Manfred Heller Thomas Geiser Amiq Gazdhar Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro International Journal of Molecular Sciences lung fibrosis macrophages induced pluripotent stem cells stem cells secretome lung repair and regeneration |
| title | Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro |
| title_full | Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro |
| title_fullStr | Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro |
| title_full_unstemmed | Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro |
| title_short | Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro |
| title_sort | interactome analysis of ipsc secretome and its effect on macrophages in vitro |
| topic | lung fibrosis macrophages induced pluripotent stem cells stem cells secretome lung repair and regeneration |
| url | https://www.mdpi.com/1422-0067/22/2/958 |
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