Pumilio2 Promotes Growth of Mature Neurons
RNA-binding proteins (RBPs) are essential regulators controlling both the cellular transcriptome and translatome. These processes enable cellular plasticity, an important prerequisite for growth. Cellular growth is a complex, tightly controlled process. Using cancer cells as model, we looked for RBP...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8998 |
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| author | Rico Schieweck Elisa-Charlott Schöneweiss Max Harner Daniela Rieger Christin Illig Barbara Saccà Bastian Popper Michael A. Kiebler |
| author_facet | Rico Schieweck Elisa-Charlott Schöneweiss Max Harner Daniela Rieger Christin Illig Barbara Saccà Bastian Popper Michael A. Kiebler |
| author_sort | Rico Schieweck |
| collection | DOAJ |
| description | RNA-binding proteins (RBPs) are essential regulators controlling both the cellular transcriptome and translatome. These processes enable cellular plasticity, an important prerequisite for growth. Cellular growth is a complex, tightly controlled process. Using cancer cells as model, we looked for RBPs displaying strong expression in published transcriptome datasets. Interestingly, we found the Pumilio (Pum) protein family to be highly expressed in all these cells. Moreover, we observed that Pum2 is regulated by basic fibroblast growth factor (bFGF). bFGF selectively enhances protein levels of Pum2 and the eukaryotic initiation factor 4E (eIF4E). Exploiting atomic force microscopy and in vitro pulldown assays, we show that Pum2 selects for <i>eIF4E</i> mRNA binding. Loss of Pum2 reduces eIF4E translation. Accordingly, depletion of Pum2 led to decreased soma size and dendritic branching of mature neurons, which was accompanied by a reduction in essential growth factors. In conclusion, we identify Pum2 as an important growth factor for mature neurons. Consequently, it is tempting to speculate that Pum2 may promote cancer growth. |
| first_indexed | 2024-03-10T08:44:23Z |
| format | Article |
| id | doaj.art-eb5e55f424c740aaaaa93a4ab1fb08d7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:23Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eb5e55f424c740aaaaa93a4ab1fb08d72023-11-22T08:03:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216899810.3390/ijms22168998Pumilio2 Promotes Growth of Mature NeuronsRico Schieweck0Elisa-Charlott Schöneweiss1Max Harner2Daniela Rieger3Christin Illig4Barbara Saccà5Bastian Popper6Michael A. Kiebler7Biomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyZentrum für Medizinische Biotechnologie (ZMB), University of Duisburg-Essen, 41541 Duisburg, GermanyBiomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyBiomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyBiomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyZentrum für Medizinische Biotechnologie (ZMB), University of Duisburg-Essen, 41541 Duisburg, GermanyBiomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyBiomedical Center (BMC), Department for Cell Biology & Anatomy, Medical Faculty, Ludwig-Maximilians-University, 82152 München, GermanyRNA-binding proteins (RBPs) are essential regulators controlling both the cellular transcriptome and translatome. These processes enable cellular plasticity, an important prerequisite for growth. Cellular growth is a complex, tightly controlled process. Using cancer cells as model, we looked for RBPs displaying strong expression in published transcriptome datasets. Interestingly, we found the Pumilio (Pum) protein family to be highly expressed in all these cells. Moreover, we observed that Pum2 is regulated by basic fibroblast growth factor (bFGF). bFGF selectively enhances protein levels of Pum2 and the eukaryotic initiation factor 4E (eIF4E). Exploiting atomic force microscopy and in vitro pulldown assays, we show that Pum2 selects for <i>eIF4E</i> mRNA binding. Loss of Pum2 reduces eIF4E translation. Accordingly, depletion of Pum2 led to decreased soma size and dendritic branching of mature neurons, which was accompanied by a reduction in essential growth factors. In conclusion, we identify Pum2 as an important growth factor for mature neurons. Consequently, it is tempting to speculate that Pum2 may promote cancer growth.https://www.mdpi.com/1422-0067/22/16/8998Pumilio2neuronal growtheIF4Eatomic force microscopy |
| spellingShingle | Rico Schieweck Elisa-Charlott Schöneweiss Max Harner Daniela Rieger Christin Illig Barbara Saccà Bastian Popper Michael A. Kiebler Pumilio2 Promotes Growth of Mature Neurons International Journal of Molecular Sciences Pumilio2 neuronal growth eIF4E atomic force microscopy |
| title | Pumilio2 Promotes Growth of Mature Neurons |
| title_full | Pumilio2 Promotes Growth of Mature Neurons |
| title_fullStr | Pumilio2 Promotes Growth of Mature Neurons |
| title_full_unstemmed | Pumilio2 Promotes Growth of Mature Neurons |
| title_short | Pumilio2 Promotes Growth of Mature Neurons |
| title_sort | pumilio2 promotes growth of mature neurons |
| topic | Pumilio2 neuronal growth eIF4E atomic force microscopy |
| url | https://www.mdpi.com/1422-0067/22/16/8998 |
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