Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis
Long-chain fatty acids (LCFAs) serve as energy sources, components of cell membranes, and precursors for signaling molecules. Uremia alters LCFA metabolism so that the risk of cardiovascular events in chronic kidney disease (CKD) is increased. End-stage renal disease (ESRD) patients undergoing dialy...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/3/269 |
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| author | Tong Liu Inci Dogan Michael Rothe Jana Reichardt Felix Knauf Maik Gollasch Friedrich C. Luft Benjamin Gollasch |
| author_facet | Tong Liu Inci Dogan Michael Rothe Jana Reichardt Felix Knauf Maik Gollasch Friedrich C. Luft Benjamin Gollasch |
| author_sort | Tong Liu |
| collection | DOAJ |
| description | Long-chain fatty acids (LCFAs) serve as energy sources, components of cell membranes, and precursors for signaling molecules. Uremia alters LCFA metabolism so that the risk of cardiovascular events in chronic kidney disease (CKD) is increased. End-stage renal disease (ESRD) patients undergoing dialysis are particularly affected and their hemodialysis (HD) treatment could influence blood LCFA bioaccumulation and transformation. We investigated blood LCFA in HD patients and studied LCFA profiles in vivo by analyzing arterio–venous (A–V) LFCA differences in upper limbs. We collected arterial and venous blood samples from 12 ESRD patients, before and after HD, and analyzed total LCFA levels in red blood cells (RBCs) and plasma by LC–MS/MS tandem mass spectrometry. We observed that differences in arterial and venous LFCA contents within RBCs (RBC LCFA A–V differences) were affected by HD treatment. Numerous saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) n-6 showed negative A–V differences, accumulated during peripheral tissue perfusion of the upper limbs, in RBCs before HD. HD reduced these differences. The omega-3 quotient in the erythrocyte membranes was not affected by HD in either arterial or venous blood. Our data demonstrate that A–V differences in fatty acids status of LCFA are present and active in mature erythrocytes and their bioaccumulation is sensitive to single HD treatment. |
| first_indexed | 2024-03-09T13:19:55Z |
| format | Article |
| id | doaj.art-eb615ec8d07c44738657c280d00dc795 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T13:19:55Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-eb615ec8d07c44738657c280d00dc7952023-11-30T21:31:32ZengMDPI AGMetabolites2218-19892022-03-0112326910.3390/metabo12030269Bioaccumulation of Blood Long-Chain Fatty Acids during HemodialysisTong Liu0Inci Dogan1Michael Rothe2Jana Reichardt3Felix Knauf4Maik Gollasch5Friedrich C. Luft6Benjamin Gollasch7Experimental and Clinical Research Center (ECRC), a Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, 13125 Berlin, GermanyLipidomix, GmbH, Robert-Rössle-Straße 10, 13125 Berlin, GermanyLipidomix, GmbH, Robert-Rössle-Straße 10, 13125 Berlin, GermanyDepartment of Nephrology and Medical Intensive Care, Charité—Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Nephrology and Medical Intensive Care, Charité—Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyExperimental and Clinical Research Center (ECRC), a Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, 13125 Berlin, GermanyExperimental and Clinical Research Center (ECRC), a Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, 13125 Berlin, GermanyExperimental and Clinical Research Center (ECRC), a Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, 13125 Berlin, GermanyLong-chain fatty acids (LCFAs) serve as energy sources, components of cell membranes, and precursors for signaling molecules. Uremia alters LCFA metabolism so that the risk of cardiovascular events in chronic kidney disease (CKD) is increased. End-stage renal disease (ESRD) patients undergoing dialysis are particularly affected and their hemodialysis (HD) treatment could influence blood LCFA bioaccumulation and transformation. We investigated blood LCFA in HD patients and studied LCFA profiles in vivo by analyzing arterio–venous (A–V) LFCA differences in upper limbs. We collected arterial and venous blood samples from 12 ESRD patients, before and after HD, and analyzed total LCFA levels in red blood cells (RBCs) and plasma by LC–MS/MS tandem mass spectrometry. We observed that differences in arterial and venous LFCA contents within RBCs (RBC LCFA A–V differences) were affected by HD treatment. Numerous saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) n-6 showed negative A–V differences, accumulated during peripheral tissue perfusion of the upper limbs, in RBCs before HD. HD reduced these differences. The omega-3 quotient in the erythrocyte membranes was not affected by HD in either arterial or venous blood. Our data demonstrate that A–V differences in fatty acids status of LCFA are present and active in mature erythrocytes and their bioaccumulation is sensitive to single HD treatment.https://www.mdpi.com/2218-1989/12/3/269exerciselipidomicserythrocytesfatty acidschronic kidney diseasehemodialysis |
| spellingShingle | Tong Liu Inci Dogan Michael Rothe Jana Reichardt Felix Knauf Maik Gollasch Friedrich C. Luft Benjamin Gollasch Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis Metabolites exercise lipidomics erythrocytes fatty acids chronic kidney disease hemodialysis |
| title | Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis |
| title_full | Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis |
| title_fullStr | Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis |
| title_full_unstemmed | Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis |
| title_short | Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis |
| title_sort | bioaccumulation of blood long chain fatty acids during hemodialysis |
| topic | exercise lipidomics erythrocytes fatty acids chronic kidney disease hemodialysis |
| url | https://www.mdpi.com/2218-1989/12/3/269 |
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