Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury
Abstract Introduction Electroacupuncture protects neurons and myelinated axons after spinal cord injury by mitigating the inflammatory response and oxidative stress, but how it exerts these effects is unclear. Methods and results Spinal cord injury was induced in C57BL/6 wild‐type and apolipoprotein...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-09-01
|
| Series: | Brain and Behavior |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/brb3.2328 |
| _version_ | 1828916123934916608 |
|---|---|
| author | Ni Dai Chenglin Tang Hui Liu Siqin Huang |
| author_facet | Ni Dai Chenglin Tang Hui Liu Siqin Huang |
| author_sort | Ni Dai |
| collection | DOAJ |
| description | Abstract Introduction Electroacupuncture protects neurons and myelinated axons after spinal cord injury by mitigating the inflammatory response and oxidative stress, but how it exerts these effects is unclear. Methods and results Spinal cord injury was induced in C57BL/6 wild‐type and apolipoprotein E (ApoE) knockout (ApoE–/–) mice, followed by electroacupuncture or ApoE mimetic peptide COG112 treatment. Mice with spinal cord injury suffered loss of myelinated axons and hindlimb motor function through the detections of Basso mouse scale, histology, and transmission electron microscopy; electroacupuncture partially reversed these effects in wild‐type mice but not in ApoE–/– mice. Combining exogenous ApoE administration with electroacupuncture significantly mitigated the effects of spinal cord injury in both mouse strains, and these effects were associated with up‐regulation of anti‐inflammatory cytokines and down‐regulation of pro‐inflammatory cytokines which were detected by quantitative reverse transcription‐polymerase chain reaction. Combination treatment also reduced oxidative stress by up‐regulating ApoE and Nrf2/HO‐1 signaling pathway through the detections of immunofluorescence and western blot analysis. Conclusions These results suggest that electroacupuncture protects neurons and myelinated axons following spinal cord injury through an ApoE‐dependent mechanism. |
| first_indexed | 2024-12-13T20:26:58Z |
| format | Article |
| id | doaj.art-eb61fcfee1f1435f866a53f8ccb86895 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-12-13T20:26:58Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-eb61fcfee1f1435f866a53f8ccb868952022-12-21T23:32:32ZengWileyBrain and Behavior2162-32792021-09-01119n/an/a10.1002/brb3.2328Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injuryNi Dai0Chenglin Tang1Hui Liu2Siqin Huang3Traditional Chinese Medicine College Chongqing Medical University Chongqing ChinaTraditional Chinese Medicine College Chongqing Medical University Chongqing ChinaInstitute of Neuroscience Chongqing Medical University Chongqing ChinaTraditional Chinese Medicine College Chongqing Medical University Chongqing ChinaAbstract Introduction Electroacupuncture protects neurons and myelinated axons after spinal cord injury by mitigating the inflammatory response and oxidative stress, but how it exerts these effects is unclear. Methods and results Spinal cord injury was induced in C57BL/6 wild‐type and apolipoprotein E (ApoE) knockout (ApoE–/–) mice, followed by electroacupuncture or ApoE mimetic peptide COG112 treatment. Mice with spinal cord injury suffered loss of myelinated axons and hindlimb motor function through the detections of Basso mouse scale, histology, and transmission electron microscopy; electroacupuncture partially reversed these effects in wild‐type mice but not in ApoE–/– mice. Combining exogenous ApoE administration with electroacupuncture significantly mitigated the effects of spinal cord injury in both mouse strains, and these effects were associated with up‐regulation of anti‐inflammatory cytokines and down‐regulation of pro‐inflammatory cytokines which were detected by quantitative reverse transcription‐polymerase chain reaction. Combination treatment also reduced oxidative stress by up‐regulating ApoE and Nrf2/HO‐1 signaling pathway through the detections of immunofluorescence and western blot analysis. Conclusions These results suggest that electroacupuncture protects neurons and myelinated axons following spinal cord injury through an ApoE‐dependent mechanism.https://doi.org/10.1002/brb3.2328apolipoprotein Eelectroacupunctureinflammatory responsenuclear factor erythroid 2‐related factor 2oxidative stressspinal cord injury |
| spellingShingle | Ni Dai Chenglin Tang Hui Liu Siqin Huang Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury Brain and Behavior apolipoprotein E electroacupuncture inflammatory response nuclear factor erythroid 2‐related factor 2 oxidative stress spinal cord injury |
| title | Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury |
| title_full | Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury |
| title_fullStr | Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury |
| title_full_unstemmed | Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury |
| title_short | Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury |
| title_sort | effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating apoe and nrf2 in a mouse model of spinal cord injury |
| topic | apolipoprotein E electroacupuncture inflammatory response nuclear factor erythroid 2‐related factor 2 oxidative stress spinal cord injury |
| url | https://doi.org/10.1002/brb3.2328 |
| work_keys_str_mv | AT nidai effectofelectroacupunctureoninhibitionofinflammatoryresponseandoxidativestressthroughactivatingapoeandnrf2inamousemodelofspinalcordinjury AT chenglintang effectofelectroacupunctureoninhibitionofinflammatoryresponseandoxidativestressthroughactivatingapoeandnrf2inamousemodelofspinalcordinjury AT huiliu effectofelectroacupunctureoninhibitionofinflammatoryresponseandoxidativestressthroughactivatingapoeandnrf2inamousemodelofspinalcordinjury AT siqinhuang effectofelectroacupunctureoninhibitionofinflammatoryresponseandoxidativestressthroughactivatingapoeandnrf2inamousemodelofspinalcordinjury |