The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study)
Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using po...
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| Format: | Article |
| Language: | English |
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Universidad de Cordoba
2015-01-01
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| Series: | Revista MVZ Cordoba |
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| Online Access: | http://revistas.unicordoba.edu.co/index.php/revistamvz/article/view/78 |
| _version_ | 1818938847829950464 |
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| author | Rosa Gagliardi B Diana Martínez A Beatriz Tellechea H Pedro Sitjar Q Silvia Llambí D María Arruga L |
| author_facet | Rosa Gagliardi B Diana Martínez A Beatriz Tellechea H Pedro Sitjar Q Silvia Llambí D María Arruga L |
| author_sort | Rosa Gagliardi B |
| collection | DOAJ |
| description | Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using potassium acetate with a subsequent degradation from RNA with RNAsaH. The concentration and quality of the DNA obtained was evaluated with a Nanodrop, ND-1000 spectrophotometer. To determine the presence or absence of the mdr1-1Δ mutation, DNA samples were sent to Gene Seek, Neogen Corporation of Chicago, United States, for genotyping. Results. In all 95 animals studied, the mdr1-1Δ mutation was not present. Conclusions. Based on the preliminary results obtained, other elements that may cause adverse drug reactions must be considered: unidentified mutations in other regions of the MDR1 gene; mutations in other genes involved in the transport of drugs from the same subfamily or another; mutations in enzymes involved in drug metabolism (e.g. Cytochrome P450). Moreover, especially with Border Collies and Greyhounds, it is advisable to increase the number of animals in the study.
Key words: Dogs, ivermectine, P-glycoprotein (Source: CAB). |
| first_indexed | 2024-12-20T06:14:22Z |
| format | Article |
| id | doaj.art-eb6a621caa6540109e26de8c7309afb4 |
| institution | Directory Open Access Journal |
| issn | 0122-0268 1909-0544 |
| language | English |
| last_indexed | 2024-12-20T06:14:22Z |
| publishDate | 2015-01-01 |
| publisher | Universidad de Cordoba |
| record_format | Article |
| series | Revista MVZ Cordoba |
| spelling | doaj.art-eb6a621caa6540109e26de8c7309afb42022-12-21T19:50:34ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442015-01-0120110.21897/rmvz.78The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study)Rosa Gagliardi B0Diana Martínez A1Beatriz Tellechea H2Pedro Sitjar Q3Silvia Llambí D4María Arruga L5Universidad de la República, Facultad de Veterinaria, Área Genética. Montevideo, C.P. 11600, UruguayVeterinarios con ejercicio liberal de la profesión.Veterinarios con ejercicio liberal de la profesión.Veterinarios con ejercicio liberal de la profesión.Universidad de la República, Facultad de Veterinaria, Área Genética. Montevideo, C.P. 11600, UruguayUniversidad de Zaragoza, Facultad de Veterinaria, Laboratorio de Citogenética y Genética Molecular. Zaragoza, C. P. 50013, España.Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using potassium acetate with a subsequent degradation from RNA with RNAsaH. The concentration and quality of the DNA obtained was evaluated with a Nanodrop, ND-1000 spectrophotometer. To determine the presence or absence of the mdr1-1Δ mutation, DNA samples were sent to Gene Seek, Neogen Corporation of Chicago, United States, for genotyping. Results. In all 95 animals studied, the mdr1-1Δ mutation was not present. Conclusions. Based on the preliminary results obtained, other elements that may cause adverse drug reactions must be considered: unidentified mutations in other regions of the MDR1 gene; mutations in other genes involved in the transport of drugs from the same subfamily or another; mutations in enzymes involved in drug metabolism (e.g. Cytochrome P450). Moreover, especially with Border Collies and Greyhounds, it is advisable to increase the number of animals in the study. Key words: Dogs, ivermectine, P-glycoprotein (Source: CAB).http://revistas.unicordoba.edu.co/index.php/revistamvz/article/view/78DogsivermectineP-glycoprotein |
| spellingShingle | Rosa Gagliardi B Diana Martínez A Beatriz Tellechea H Pedro Sitjar Q Silvia Llambí D María Arruga L The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) Revista MVZ Cordoba Dogs ivermectine P-glycoprotein |
| title | The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) |
| title_full | The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) |
| title_fullStr | The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) |
| title_full_unstemmed | The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) |
| title_short | The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study) |
| title_sort | search for the mdr1 1δ mutation of the mdr1 gene in four canine breeds in uruguay preliminary study |
| topic | Dogs ivermectine P-glycoprotein |
| url | http://revistas.unicordoba.edu.co/index.php/revistamvz/article/view/78 |
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