Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action
Estradiol (E2) treatment has been known to induce changes in food intake, energy expenditure, and weight gain. However, its direct effects on adipose tissue macrophages (ATM) in vivo are not fully understood. Thus, we aimed to explore this aspect at cellular and molecular levels in ovariectomized ob...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-09-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2022.2128871 |
| _version_ | 1798030223897264128 |
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| author | Kyeong-dae Kim Jeong Min Choe Soomin Myoung Seung Hyun Lee Minkyu Kim Jae-Hoon Choi Hyun Tae Park |
| author_facet | Kyeong-dae Kim Jeong Min Choe Soomin Myoung Seung Hyun Lee Minkyu Kim Jae-Hoon Choi Hyun Tae Park |
| author_sort | Kyeong-dae Kim |
| collection | DOAJ |
| description | Estradiol (E2) treatment has been known to induce changes in food intake, energy expenditure, and weight gain. However, its direct effects on adipose tissue macrophages (ATM) in vivo are not fully understood. Thus, we aimed to explore this aspect at cellular and molecular levels in ovariectomized obese mice. We examined the changes in ATMs after eight weeks of a high-fat diet (HFD) in male, female, and ovariectomized (OVX) mice. After eight weeks, osmotic pumps were inserted into OVX mice to provide two weeks of E2 treatment. We additionally set up a vehicle Pair-Fed (PF) control group that supplied the same amount of HFD consumed by the E2-treated group. We then investigated the in vivo phenotypic changes of visceral adipose tissue (VAT) macrophages. The percentage of M1-like ATMs decreased by the anorectic effect of E2, while M2-like ATMs increased regardless of the anorexia. E2 treatment increased the expression of anti-inflammatory genes but decreased pro-inflammatory genes in VAT. Monocyte recruitment and local proliferation contributed to M2-like ATMs. Furthermore, M2-like phenotypes were induced by E2 treatment in human macrophages. E2 treatment increases M2-like macrophages and improves the tissue milieu of VAT regardless of the anorectic reaction of E2. |
| first_indexed | 2024-04-11T19:37:51Z |
| format | Article |
| id | doaj.art-eb6c2d31b9a14c289df7e709744dd362 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-04-11T19:37:51Z |
| publishDate | 2022-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-eb6c2d31b9a14c289df7e709744dd3622022-12-22T04:06:48ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852022-09-0126524325310.1080/19768354.2022.2128871Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic actionKyeong-dae Kim0Jeong Min Choe1Soomin Myoung2Seung Hyun Lee3Minkyu Kim4Jae-Hoon Choi5Hyun Tae Park6Department of Life Science, College of Natural Sciences, Research Institute for Natural Sciences, Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Republic of KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Republic of KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Republic of KoreaDepartment of Life Science, College of Natural Sciences, Research Institute for Natural Sciences, Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Republic of KoreaDepartment of Life Science, College of Natural Sciences, Research Institute for Natural Sciences, Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Republic of KoreaDepartment of Life Science, College of Natural Sciences, Research Institute for Natural Sciences, Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Republic of KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Korea University, Seoul, Republic of KoreaEstradiol (E2) treatment has been known to induce changes in food intake, energy expenditure, and weight gain. However, its direct effects on adipose tissue macrophages (ATM) in vivo are not fully understood. Thus, we aimed to explore this aspect at cellular and molecular levels in ovariectomized obese mice. We examined the changes in ATMs after eight weeks of a high-fat diet (HFD) in male, female, and ovariectomized (OVX) mice. After eight weeks, osmotic pumps were inserted into OVX mice to provide two weeks of E2 treatment. We additionally set up a vehicle Pair-Fed (PF) control group that supplied the same amount of HFD consumed by the E2-treated group. We then investigated the in vivo phenotypic changes of visceral adipose tissue (VAT) macrophages. The percentage of M1-like ATMs decreased by the anorectic effect of E2, while M2-like ATMs increased regardless of the anorexia. E2 treatment increased the expression of anti-inflammatory genes but decreased pro-inflammatory genes in VAT. Monocyte recruitment and local proliferation contributed to M2-like ATMs. Furthermore, M2-like phenotypes were induced by E2 treatment in human macrophages. E2 treatment increases M2-like macrophages and improves the tissue milieu of VAT regardless of the anorectic reaction of E2.https://www.tandfonline.com/doi/10.1080/19768354.2022.2128871Adipose tissueappetite depressantestradiolmacrophageobesity |
| spellingShingle | Kyeong-dae Kim Jeong Min Choe Soomin Myoung Seung Hyun Lee Minkyu Kim Jae-Hoon Choi Hyun Tae Park Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action Animal Cells and Systems Adipose tissue appetite depressant estradiol macrophage obesity |
| title | Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| title_full | Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| title_fullStr | Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| title_full_unstemmed | Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| title_short | Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| title_sort | estradiol treatment increases m2 like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action |
| topic | Adipose tissue appetite depressant estradiol macrophage obesity |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2022.2128871 |
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