β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction
Uncontrollable stress exposure impairs working memory and reduces the firing of dorsolateral prefrontal cortex (dlPFC) “Delay cells”, involving high levels of norepinephrine and dopamine release. Previous work has focused on catecholamine actions on dlPFC pyramidal cells, but inhibitory interneurons...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Neurobiology of Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352289524000249 |
| _version_ | 1797249183823953920 |
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| author | M.K.P. Joyce S. Yang K. Morin A. Duque J. Arellano D. Datta M. Wang A.F.T. Arnsten |
| author_facet | M.K.P. Joyce S. Yang K. Morin A. Duque J. Arellano D. Datta M. Wang A.F.T. Arnsten |
| author_sort | M.K.P. Joyce |
| collection | DOAJ |
| description | Uncontrollable stress exposure impairs working memory and reduces the firing of dorsolateral prefrontal cortex (dlPFC) “Delay cells”, involving high levels of norepinephrine and dopamine release. Previous work has focused on catecholamine actions on dlPFC pyramidal cells, but inhibitory interneurons may contribute as well. The current study combined immunohistochemistry and multi-scale microscopy with iontophoretic physiology and behavioral analyses to examine the effects of beta1-noradrenergic receptors (β1-ARs) on inhibitory neurons in layer III dlPFC. We found β1-AR robustly expressed on different classes of inhibitory neurons labeled by the calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV). Immunoelectron microscopy confirmed β1-AR expression on the plasma membrane of PV-expressing dendrites. PV interneurons can be identified as fast-spiking (FS) in physiological recordings, and thus were studied in macaques performing a working memory task. Iontophoresis of a β1-AR agonist had a mixed effect, increasing the firing of a subset and decreasing the firing of others, likely reflecting loss of firing of the entire microcircuit. This loss of overall firing likely contributes to impaired working memory during stress, as pretreatment with the selective β1-AR antagonist, nebivolol, prevented stress-induced working memory deficits. Thus, selective β1-AR antagonists may be helpful in treating stress-related disorders. |
| first_indexed | 2024-04-24T20:26:26Z |
| format | Article |
| id | doaj.art-eb6ec7a3a9e3403baf13a4f089ed8707 |
| institution | Directory Open Access Journal |
| issn | 2352-2895 |
| language | English |
| last_indexed | 2024-04-24T20:26:26Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Stress |
| spelling | doaj.art-eb6ec7a3a9e3403baf13a4f089ed87072024-03-22T05:39:53ZengElsevierNeurobiology of Stress2352-28952024-05-0130100628β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunctionM.K.P. Joyce0S. Yang1K. Morin2A. Duque3J. Arellano4D. Datta5M. Wang6A.F.T. Arnsten7Dept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USADept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USACorresponding author. Dept. Neuroscience, Yale Medical School, 333 Cedar St. New Haven, CT, 06510, USA.; Dept. Neuroscience, Yale Medical School, New Haven, CT, 06510, USAUncontrollable stress exposure impairs working memory and reduces the firing of dorsolateral prefrontal cortex (dlPFC) “Delay cells”, involving high levels of norepinephrine and dopamine release. Previous work has focused on catecholamine actions on dlPFC pyramidal cells, but inhibitory interneurons may contribute as well. The current study combined immunohistochemistry and multi-scale microscopy with iontophoretic physiology and behavioral analyses to examine the effects of beta1-noradrenergic receptors (β1-ARs) on inhibitory neurons in layer III dlPFC. We found β1-AR robustly expressed on different classes of inhibitory neurons labeled by the calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV). Immunoelectron microscopy confirmed β1-AR expression on the plasma membrane of PV-expressing dendrites. PV interneurons can be identified as fast-spiking (FS) in physiological recordings, and thus were studied in macaques performing a working memory task. Iontophoresis of a β1-AR agonist had a mixed effect, increasing the firing of a subset and decreasing the firing of others, likely reflecting loss of firing of the entire microcircuit. This loss of overall firing likely contributes to impaired working memory during stress, as pretreatment with the selective β1-AR antagonist, nebivolol, prevented stress-induced working memory deficits. Thus, selective β1-AR antagonists may be helpful in treating stress-related disorders.http://www.sciencedirect.com/science/article/pii/S2352289524000249NorepinephrineStressPrefrontal cortexWorking memoryBeta-adrenergic receptorsADRAB1 |
| spellingShingle | M.K.P. Joyce S. Yang K. Morin A. Duque J. Arellano D. Datta M. Wang A.F.T. Arnsten β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction Neurobiology of Stress Norepinephrine Stress Prefrontal cortex Working memory Beta-adrenergic receptors ADRAB1 |
| title | β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction |
| title_full | β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction |
| title_fullStr | β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction |
| title_full_unstemmed | β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction |
| title_short | β1-adrenoceptor expression on GABAergic interneurons in primate dorsolateral prefrontal cortex: potential role in stress-induced cognitive dysfunction |
| title_sort | β1 adrenoceptor expression on gabaergic interneurons in primate dorsolateral prefrontal cortex potential role in stress induced cognitive dysfunction |
| topic | Norepinephrine Stress Prefrontal cortex Working memory Beta-adrenergic receptors ADRAB1 |
| url | http://www.sciencedirect.com/science/article/pii/S2352289524000249 |
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