Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens
ABSTRACT: Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world....
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912300648X |
| _version_ | 1827593761486536704 |
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| author | Juan Jaton Evangelina Gómez María Soledad Lucero María José Gravisaco Silvina Pinto Ariel Vagnozzi María Isabel Craig Sebastián Di Giacomo Analía Berinstein Silvina Chimeno Zoth |
| author_facet | Juan Jaton Evangelina Gómez María Soledad Lucero María José Gravisaco Silvina Pinto Ariel Vagnozzi María Isabel Craig Sebastián Di Giacomo Analía Berinstein Silvina Chimeno Zoth |
| author_sort | Juan Jaton |
| collection | DOAJ |
| description | ABSTRACT: Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world. IBDV infects immature B lymphocytes, affecting the immune status of birds and facilitating infections by other pathogens such as avian infectious bronchitis virus (IBV). Although it has been reported that the interaction between these viruses increases IBV clinical signs, there are no actual studies about the interaction between regional circulating isolates that validate this statement. In this context, the objective of our work was to evaluate the effect of the interaction between local isolates of IBDV (belonging to genogroup 4) and IBV (lineage GI-16) in chickens. Thus, specific pathogen-free chickens were orally inoculated with IBDV genogroup (G) 4 or with PBS at 5 d of age. At 14-days postinoculation (dpi) the animals were intratracheally inoculated with a GI-16 IBV or with PBS. At multiple time points, groups of birds were euthanized and different parameters such as histological damage, viral load, lymphocyte populations and specific antibodies were evaluated. The success of IBDV infection was confirmed by the severity of bursal atrophy, viral detection, and presence of anti-IBDV antibodies. In IBV-infected animals, the presence of viral genome was detected in both kidney and bursa. The coinfected animals showed higher degree of lymphocyte infiltration in kidney, higher rate of animals with IBV viral genome in bursa at 28 dpi, and a clear decrease in antibody response against IBV at 28, 35, and 40 dpi. The results indicate that the infection with the local isolate of IBDV affects the immune status of the chickens, causing major severe damage, in response to IBV infection, which could consequently severely affect the local poultry industry. |
| first_indexed | 2024-03-09T02:16:20Z |
| format | Article |
| id | doaj.art-eb6f6ad3a689462b8702bcc9ec8043d5 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-09T02:16:20Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-eb6f6ad3a689462b8702bcc9ec8043d52023-12-07T05:27:18ZengElsevierPoultry Science0032-57912023-12-0110212103129Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickensJuan Jaton0Evangelina Gómez1María Soledad Lucero2María José Gravisaco3Silvina Pinto4Ariel Vagnozzi5María Isabel Craig6Sebastián Di Giacomo7Analía Berinstein8Silvina Chimeno Zoth9Laboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, ArgentinaLaboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, ArgentinaLaboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, ArgentinaLaboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, ArgentinaDepartment of Pathology, Faculty of Veterinary Sciences, University of Buenos Aires, Buenos Aires, ArgentinaPoultry Laboratory, Institute of Virology and Technological Innovations, INTA-CONICET, Buenos Aires, ArgentinaPoultry Laboratory, Institute of Virology and Technological Innovations, INTA-CONICET, Buenos Aires, ArgentinaPoultry Laboratory, Institute of Virology and Technological Innovations, INTA-CONICET, Buenos Aires, ArgentinaLaboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, ArgentinaLaboratory of Avian Immunology and Vaccines, Institute of Agrobiotechnology and Molecular Biology, INTA-CONICET, Buenos Aires, Argentina; Corresponding author:ABSTRACT: Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world. IBDV infects immature B lymphocytes, affecting the immune status of birds and facilitating infections by other pathogens such as avian infectious bronchitis virus (IBV). Although it has been reported that the interaction between these viruses increases IBV clinical signs, there are no actual studies about the interaction between regional circulating isolates that validate this statement. In this context, the objective of our work was to evaluate the effect of the interaction between local isolates of IBDV (belonging to genogroup 4) and IBV (lineage GI-16) in chickens. Thus, specific pathogen-free chickens were orally inoculated with IBDV genogroup (G) 4 or with PBS at 5 d of age. At 14-days postinoculation (dpi) the animals were intratracheally inoculated with a GI-16 IBV or with PBS. At multiple time points, groups of birds were euthanized and different parameters such as histological damage, viral load, lymphocyte populations and specific antibodies were evaluated. The success of IBDV infection was confirmed by the severity of bursal atrophy, viral detection, and presence of anti-IBDV antibodies. In IBV-infected animals, the presence of viral genome was detected in both kidney and bursa. The coinfected animals showed higher degree of lymphocyte infiltration in kidney, higher rate of animals with IBV viral genome in bursa at 28 dpi, and a clear decrease in antibody response against IBV at 28, 35, and 40 dpi. The results indicate that the infection with the local isolate of IBDV affects the immune status of the chickens, causing major severe damage, in response to IBV infection, which could consequently severely affect the local poultry industry.http://www.sciencedirect.com/science/article/pii/S003257912300648XcoinfectionIBDVIBVspecific pathogen-free chickenlocal strain |
| spellingShingle | Juan Jaton Evangelina Gómez María Soledad Lucero María José Gravisaco Silvina Pinto Ariel Vagnozzi María Isabel Craig Sebastián Di Giacomo Analía Berinstein Silvina Chimeno Zoth Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens Poultry Science coinfection IBDV IBV specific pathogen-free chicken local strain |
| title | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_full | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_fullStr | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_full_unstemmed | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_short | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_sort | study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen free chickens |
| topic | coinfection IBDV IBV specific pathogen-free chicken local strain |
| url | http://www.sciencedirect.com/science/article/pii/S003257912300648X |
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