Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake
Viruses in aquatic ecosystems exhibit remarkable abundance and diversity. However, scattered studies have been conducted to mine uncultured viruses and identify them taxonomically in lake water. Here, whole genomes (29–173 kbp) of seven uncultured dsDNA bacteriophages were discovered in Dishui Lake,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/10/2038 |
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| author | Haoyun Cai Yifan Zhou Xiefei Li Tianqi Xu Yimin Ni Shuang Wu Yongxin Yu Yongjie Wang |
| author_facet | Haoyun Cai Yifan Zhou Xiefei Li Tianqi Xu Yimin Ni Shuang Wu Yongxin Yu Yongjie Wang |
| author_sort | Haoyun Cai |
| collection | DOAJ |
| description | Viruses in aquatic ecosystems exhibit remarkable abundance and diversity. However, scattered studies have been conducted to mine uncultured viruses and identify them taxonomically in lake water. Here, whole genomes (29–173 kbp) of seven uncultured dsDNA bacteriophages were discovered in Dishui Lake, the largest artificial lake in Shanghai. We analyzed their genomic signatures and found a series of viral auxiliary metabolic genes closely associated with protein synthesis and host metabolism. Dishui Lake phages shared more genes with uncultivated environmental viruses than with reference viruses based on the gene-sharing network classification. Phylogeny of proteomes and comparative genomics delineated three new genera within two known viral families of <i>Kyanoviridae</i> and <i>Autographiviridae</i>, and four new families in <i>Caudoviricetes</i> for these seven novel phages. Their potential hosts appeared to be from the dominant bacterial phyla in Dishui Lake. Altogether, our study provides initial insights into the composition and diversity of bacteriophage communities in Dishui Lake, contributing valuable knowledge to the ongoing research on the roles played by viruses in freshwater ecosystems. |
| first_indexed | 2024-03-10T20:50:21Z |
| format | Article |
| id | doaj.art-eb711098fb5c4f70b0d02bd3a2bb7a37 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:50:21Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-eb711098fb5c4f70b0d02bd3a2bb7a372023-11-19T18:27:19ZengMDPI AGViruses1999-49152023-09-011510203810.3390/v15102038Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui LakeHaoyun Cai0Yifan Zhou1Xiefei Li2Tianqi Xu3Yimin Ni4Shuang Wu5Yongxin Yu6Yongjie Wang7College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaViruses in aquatic ecosystems exhibit remarkable abundance and diversity. However, scattered studies have been conducted to mine uncultured viruses and identify them taxonomically in lake water. Here, whole genomes (29–173 kbp) of seven uncultured dsDNA bacteriophages were discovered in Dishui Lake, the largest artificial lake in Shanghai. We analyzed their genomic signatures and found a series of viral auxiliary metabolic genes closely associated with protein synthesis and host metabolism. Dishui Lake phages shared more genes with uncultivated environmental viruses than with reference viruses based on the gene-sharing network classification. Phylogeny of proteomes and comparative genomics delineated three new genera within two known viral families of <i>Kyanoviridae</i> and <i>Autographiviridae</i>, and four new families in <i>Caudoviricetes</i> for these seven novel phages. Their potential hosts appeared to be from the dominant bacterial phyla in Dishui Lake. Altogether, our study provides initial insights into the composition and diversity of bacteriophage communities in Dishui Lake, contributing valuable knowledge to the ongoing research on the roles played by viruses in freshwater ecosystems.https://www.mdpi.com/1999-4915/15/10/2038bacteriophagegenometaxonomyfreshwater lakevirus diversity |
| spellingShingle | Haoyun Cai Yifan Zhou Xiefei Li Tianqi Xu Yimin Ni Shuang Wu Yongxin Yu Yongjie Wang Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake Viruses bacteriophage genome taxonomy freshwater lake virus diversity |
| title | Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake |
| title_full | Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake |
| title_fullStr | Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake |
| title_full_unstemmed | Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake |
| title_short | Genomic Analysis and Taxonomic Characterization of Seven Bacteriophage Genomes Metagenomic-Assembled from the Dishui Lake |
| title_sort | genomic analysis and taxonomic characterization of seven bacteriophage genomes metagenomic assembled from the dishui lake |
| topic | bacteriophage genome taxonomy freshwater lake virus diversity |
| url | https://www.mdpi.com/1999-4915/15/10/2038 |
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