Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>)
In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that non...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-09-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/11/10/887 |
| _version_ | 1818318538823172096 |
|---|---|
| author | Brigitta Zana Gábor Kemenesi Dóra Buzás Gábor Csorba Tamás Görföl Faisal Ali Anwarali Khan Nurul Farah Diyana Ahmad Tahir Safia Zeghbib Mónika Madai Henrietta Papp Fanni Földes Péter Urbán Róbert Herczeg Gábor Endre Tóth Ferenc Jakab |
| author_facet | Brigitta Zana Gábor Kemenesi Dóra Buzás Gábor Csorba Tamás Görföl Faisal Ali Anwarali Khan Nurul Farah Diyana Ahmad Tahir Safia Zeghbib Mónika Madai Henrietta Papp Fanni Földes Péter Urbán Róbert Herczeg Gábor Endre Tóth Ferenc Jakab |
| author_sort | Brigitta Zana |
| collection | DOAJ |
| description | In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the <i>Murina aenea</i> bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the <i>M. aenea</i> bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the <i>Mobatvirus</i> genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses. |
| first_indexed | 2024-12-13T09:54:49Z |
| format | Article |
| id | doaj.art-eb74670f193c45e3a086c88a9e3c858d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T09:54:49Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-eb74670f193c45e3a086c88a9e3c858d2022-12-21T23:51:50ZengMDPI AGViruses1999-49152019-09-01111088710.3390/v11100887v11100887Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>)Brigitta Zana0Gábor Kemenesi1Dóra Buzás2Gábor Csorba3Tamás Görföl4Faisal Ali Anwarali Khan5Nurul Farah Diyana Ahmad Tahir6Safia Zeghbib7Mónika Madai8Henrietta Papp9Fanni Földes10Péter Urbán11Róbert Herczeg12Gábor Endre Tóth13Ferenc Jakab14Szentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungaryDepartment of Zoology, Hungarian Natural History Museum, 1083 Budapest, HungaryDepartment of Zoology, Hungarian Natural History Museum, 1083 Budapest, HungaryFaculty of Resource Science and Technology, UniversitiMalaysia Sarawak, Kota Samarahan 94300, MalaysiaFaculty of Resource Science and Technology, UniversitiMalaysia Sarawak, Kota Samarahan 94300, MalaysiaSzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungaryInstitute of Biology, Faculty of Sciences, University of Pécs, 7622 Pécs, HungarySzentágothai Research Centre, Bioinformatics Core Facility, Bioinformatics Research Group, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungarySzentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, HungaryIn the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the <i>Murina aenea</i> bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the <i>M. aenea</i> bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the <i>Mobatvirus</i> genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses.https://www.mdpi.com/1999-4915/11/10/887Sarawak mobatvirusMinIONTb1-Lu<i>Mobatvirus</i>one health concept |
| spellingShingle | Brigitta Zana Gábor Kemenesi Dóra Buzás Gábor Csorba Tamás Görföl Faisal Ali Anwarali Khan Nurul Farah Diyana Ahmad Tahir Safia Zeghbib Mónika Madai Henrietta Papp Fanni Földes Péter Urbán Róbert Herczeg Gábor Endre Tóth Ferenc Jakab Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) Viruses Sarawak mobatvirus MinION Tb1-Lu <i>Mobatvirus</i> one health concept |
| title | Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) |
| title_full | Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) |
| title_fullStr | Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) |
| title_full_unstemmed | Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) |
| title_short | Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (<i>Murina aenea</i>) |
| title_sort | molecular identification of a novel hantavirus in malaysian bronze tube nosed bats i murina aenea i |
| topic | Sarawak mobatvirus MinION Tb1-Lu <i>Mobatvirus</i> one health concept |
| url | https://www.mdpi.com/1999-4915/11/10/887 |
| work_keys_str_mv | AT brigittazana molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT gaborkemenesi molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT dorabuzas molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT gaborcsorba molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT tamasgorfol molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT faisalalianwaralikhan molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT nurulfarahdiyanaahmadtahir molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT safiazeghbib molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT monikamadai molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT henriettapapp molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT fannifoldes molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT peterurban molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT robertherczeg molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT gaborendretoth molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai AT ferencjakab molecularidentificationofanovelhantavirusinmalaysianbronzetubenosedbatsimurinaaeneai |