Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai)
A considerable amount of bioactive compounds have been used for the biopharmaceutical engineering to help human health and nutrition. Hairy root culture (HRC) or transgenic root is a favourable alternative technique for phytochemical production. <i>Ligularia fischeri</i> is a significant...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1586 |
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| author | Mohammad Azam Ansari Ill-Min Chung Govindasamy Rajakumar Mohammad A. Alzohairy Ahmad Almatroudi Venkatesan Gopiesh Khanna Muthu Thiruvengadam |
| author_facet | Mohammad Azam Ansari Ill-Min Chung Govindasamy Rajakumar Mohammad A. Alzohairy Ahmad Almatroudi Venkatesan Gopiesh Khanna Muthu Thiruvengadam |
| author_sort | Mohammad Azam Ansari |
| collection | DOAJ |
| description | A considerable amount of bioactive compounds have been used for the biopharmaceutical engineering to help human health and nutrition. Hairy root culture (HRC) or transgenic root is a favourable alternative technique for phytochemical production. <i>Ligularia fischeri</i> is a significant source of pharmaceutically important active compounds with an enormous range of health care applications. HRC of <i>L. fischeri</i> was developed using <i>Agrobacterium rhizogenes</i> for the production of polyphenolic compounds with antioxidant, antimicrobial, antidiabetic, anticancer and anti-inflammatory pharmaceutical activities. Hairy roots (HRs) were selected by morphological assessment, genetic and molecular analyses. The maximum accumulation of fresh mass (94.15 g/L) and dry mass (9.45 g/L) was recorded in MS liquid medium supplemented with 30 g/L sucrose at 28 days. Furthermore, HRs successfully produced numerous polyphenolic compounds, including six hydroxycinnamic acids, seven flavonols, seven hydroxybenzoic acids, vanillin, resveratrol, pyrogallol, homogentisic, and veratric acids, which were identified by UHPLC analysis. HRs produced higher total phenolic (185.65 mg/g), and flavonoid (5.25 mg/g) contents than non-transformed roots (125.55 mg/g and 3.75 mg/g). As a result of these metabolic changes, pharmaceutical activities were found higher in HRs than non-transformed roots (NTRs). The present study indicates that HRC has the potential to increase the content of beneficial polyphenolic compounds with higher potential pharmaceutical activities. To the best of our knowledge, the present study is the first report on enhancing the production of polyphenolic compounds with pharmaceutical activities from the HRCs of <i>L. fischeri</i>. |
| first_indexed | 2024-12-21T07:18:21Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T07:18:21Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-eb79de84656a403da9ea37da8ddb36dc2022-12-21T19:11:51ZengMDPI AGMolecules1420-30492019-04-01248158610.3390/molecules24081586molecules24081586Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai)Mohammad Azam Ansari0Ill-Min Chung1Govindasamy Rajakumar2Mohammad A. Alzohairy3Ahmad Almatroudi4Venkatesan Gopiesh Khanna5Muthu Thiruvengadam6Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi ArabiaDepartment of Biotechnology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Vels University, Pallavaram, Chennai 600117, Tamil Nadu, IndiaDepartment of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, KoreaA considerable amount of bioactive compounds have been used for the biopharmaceutical engineering to help human health and nutrition. Hairy root culture (HRC) or transgenic root is a favourable alternative technique for phytochemical production. <i>Ligularia fischeri</i> is a significant source of pharmaceutically important active compounds with an enormous range of health care applications. HRC of <i>L. fischeri</i> was developed using <i>Agrobacterium rhizogenes</i> for the production of polyphenolic compounds with antioxidant, antimicrobial, antidiabetic, anticancer and anti-inflammatory pharmaceutical activities. Hairy roots (HRs) were selected by morphological assessment, genetic and molecular analyses. The maximum accumulation of fresh mass (94.15 g/L) and dry mass (9.45 g/L) was recorded in MS liquid medium supplemented with 30 g/L sucrose at 28 days. Furthermore, HRs successfully produced numerous polyphenolic compounds, including six hydroxycinnamic acids, seven flavonols, seven hydroxybenzoic acids, vanillin, resveratrol, pyrogallol, homogentisic, and veratric acids, which were identified by UHPLC analysis. HRs produced higher total phenolic (185.65 mg/g), and flavonoid (5.25 mg/g) contents than non-transformed roots (125.55 mg/g and 3.75 mg/g). As a result of these metabolic changes, pharmaceutical activities were found higher in HRs than non-transformed roots (NTRs). The present study indicates that HRC has the potential to increase the content of beneficial polyphenolic compounds with higher potential pharmaceutical activities. To the best of our knowledge, the present study is the first report on enhancing the production of polyphenolic compounds with pharmaceutical activities from the HRCs of <i>L. fischeri</i>.https://www.mdpi.com/1420-3049/24/8/1586<i>Ligularia fischeri</i><i>Agrobacterium rhizogenes</i>hairy root culturespolyphenolic compoundspharmaceutical activities |
| spellingShingle | Mohammad Azam Ansari Ill-Min Chung Govindasamy Rajakumar Mohammad A. Alzohairy Ahmad Almatroudi Venkatesan Gopiesh Khanna Muthu Thiruvengadam Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) Molecules <i>Ligularia fischeri</i> <i>Agrobacterium rhizogenes</i> hairy root cultures polyphenolic compounds pharmaceutical activities |
| title | Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) |
| title_full | Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) |
| title_fullStr | Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) |
| title_full_unstemmed | Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) |
| title_short | Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures of <i>Ligularia fischeri</i> Turcz. f. <i>spiciformis</i> (Nakai) |
| title_sort | evaluation of polyphenolic compounds and pharmacological activities in hairy root cultures of i ligularia fischeri i turcz f i spiciformis i nakai |
| topic | <i>Ligularia fischeri</i> <i>Agrobacterium rhizogenes</i> hairy root cultures polyphenolic compounds pharmaceutical activities |
| url | https://www.mdpi.com/1420-3049/24/8/1586 |
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