Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i>
The main objective of this work is to evaluate the potential utility of an <i>Achillea millefolium</i> extract (yarrow extract, YE) in the control of <i>H. pylori</i> infection. The supercritical anti-solvent fractionation (SAF) process of YE allowed the obtaining of two diff...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1849 |
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| author | Marisol Villalva Jose Manuel Silvan Teresa Alarcón-Cavero David Villanueva-Bermejo Laura Jaime Susana Santoyo Adolfo J. Martinez-Rodriguez |
| author_facet | Marisol Villalva Jose Manuel Silvan Teresa Alarcón-Cavero David Villanueva-Bermejo Laura Jaime Susana Santoyo Adolfo J. Martinez-Rodriguez |
| author_sort | Marisol Villalva |
| collection | DOAJ |
| description | The main objective of this work is to evaluate the potential utility of an <i>Achillea millefolium</i> extract (yarrow extract, YE) in the control of <i>H. pylori</i> infection. The supercritical anti-solvent fractionation (SAF) process of YE allowed the obtaining of two different fractions: yarrow’s precipitated fraction (YPF), enriched in most polar phenolic compounds (luteolin-7-<i>O</i>-glucoside, luteolin, and 3,5-dicaffeoylquinic acid), and yarrow’s separator fraction (YSF), enriched in monoterpenes and sesquiterpenes, mainly containing camphor, artemisia ketone, and borneol. YE was effective in reducing reactive oxygen species (ROS) production in human gastric AGS cells by 16% to 29%, depending on the <i>H. pylori</i> strain. YPF had the highest inhibitory activity (38–40%) for ROS production. YE modulated the inflammatory response in AGS gastric cells, decreasing IL-8 production by 53% to 64%. This IL-8 inhibition also showed a strain-dependent character. YPF and YSF exhibited similar behavior, reducing IL-8 production, suggesting that both phenolic compounds and essential oils could contribute to IL-8 inhibition. YSF showed the highest antibacterial activity against <i>H. pylori</i> (6.3–7.1 log CFU reduction, depending on the strain) and lower MIC (0.08 mg/mL). Results obtained have shown that YE and SAF fractions (YPF and YSF) were effective as antioxidant, anti-inflammatory, and antibacterial agents regardless of the <i>H. pylori</i> strain characteristics. |
| first_indexed | 2024-03-09T20:50:03Z |
| format | Article |
| id | doaj.art-eb7a6b8c7c774687918f9b9d5835652c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:50:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-eb7a6b8c7c774687918f9b9d5835652c2023-11-23T22:36:44ZengMDPI AGAntioxidants2076-39212022-09-011110184910.3390/antiox11101849Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i>Marisol Villalva0Jose Manuel Silvan1Teresa Alarcón-Cavero2David Villanueva-Bermejo3Laura Jaime4Susana Santoyo5Adolfo J. Martinez-Rodriguez6Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainMicrobiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainMicrobiology Department, Hospital Universitario de La Princesa, 28006 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainMicrobiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, SpainThe main objective of this work is to evaluate the potential utility of an <i>Achillea millefolium</i> extract (yarrow extract, YE) in the control of <i>H. pylori</i> infection. The supercritical anti-solvent fractionation (SAF) process of YE allowed the obtaining of two different fractions: yarrow’s precipitated fraction (YPF), enriched in most polar phenolic compounds (luteolin-7-<i>O</i>-glucoside, luteolin, and 3,5-dicaffeoylquinic acid), and yarrow’s separator fraction (YSF), enriched in monoterpenes and sesquiterpenes, mainly containing camphor, artemisia ketone, and borneol. YE was effective in reducing reactive oxygen species (ROS) production in human gastric AGS cells by 16% to 29%, depending on the <i>H. pylori</i> strain. YPF had the highest inhibitory activity (38–40%) for ROS production. YE modulated the inflammatory response in AGS gastric cells, decreasing IL-8 production by 53% to 64%. This IL-8 inhibition also showed a strain-dependent character. YPF and YSF exhibited similar behavior, reducing IL-8 production, suggesting that both phenolic compounds and essential oils could contribute to IL-8 inhibition. YSF showed the highest antibacterial activity against <i>H. pylori</i> (6.3–7.1 log CFU reduction, depending on the strain) and lower MIC (0.08 mg/mL). Results obtained have shown that YE and SAF fractions (YPF and YSF) were effective as antioxidant, anti-inflammatory, and antibacterial agents regardless of the <i>H. pylori</i> strain characteristics.https://www.mdpi.com/2076-3921/11/10/1849<i>Achillea millefolium</i>yarrow extract<i>H. pylori</i>supercritical anti-solvent fractionationanti-inflammatory activityantioxidant activity |
| spellingShingle | Marisol Villalva Jose Manuel Silvan Teresa Alarcón-Cavero David Villanueva-Bermejo Laura Jaime Susana Santoyo Adolfo J. Martinez-Rodriguez Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> Antioxidants <i>Achillea millefolium</i> yarrow extract <i>H. pylori</i> supercritical anti-solvent fractionation anti-inflammatory activity antioxidant activity |
| title | Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> |
| title_full | Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> |
| title_fullStr | Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> |
| title_full_unstemmed | Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> |
| title_short | Antioxidant, Anti-Inflammatory, and Antibacterial Properties of an <i>Achillea millefolium</i> L. Extract and Its Fractions Obtained by Supercritical Anti-Solvent Fractionation against <i>Helicobacter pylori</i> |
| title_sort | antioxidant anti inflammatory and antibacterial properties of an i achillea millefolium i l extract and its fractions obtained by supercritical anti solvent fractionation against i helicobacter pylori i |
| topic | <i>Achillea millefolium</i> yarrow extract <i>H. pylori</i> supercritical anti-solvent fractionation anti-inflammatory activity antioxidant activity |
| url | https://www.mdpi.com/2076-3921/11/10/1849 |
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