Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System
Fluorescent bioprobes are invaluable tools for visualizing live cells and deciphering complex biological processes by targeting intracellular biomarkers without disrupting cellular functions. In addition to protein-binding concepts, fluorescent probes utilize various mechanisms, including membrane,...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/3/424 |
| _version_ | 1797239693791723520 |
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| author | Heewon Cho Na-Kyeong Hong Young-Tae Chang |
| author_facet | Heewon Cho Na-Kyeong Hong Young-Tae Chang |
| author_sort | Heewon Cho |
| collection | DOAJ |
| description | Fluorescent bioprobes are invaluable tools for visualizing live cells and deciphering complex biological processes by targeting intracellular biomarkers without disrupting cellular functions. In addition to protein-binding concepts, fluorescent probes utilize various mechanisms, including membrane, metabolism, and gating-oriented strategies. This study introduces a novel fluorescent mechanism distinct from existing ways. Here, we developed a B cell selective probe, CDrB, with unique transport mechanisms. Through SLC-CRISPRa screening, we identified two transporters, SLCO1B3 and SLC25A41, by sorting out populations exhibiting higher and lower fluorescence intensities, respectively, demonstrating contrasting activities. We confirmed that SLCO1B3, with comparable expression levels in T and B cells, facilitates the transport of CDrB into cells, while SLC25A41, overexpressed in T lymphocytes, actively exports CDrB. This observation suggests that SLC25A41 plays a crucial role in discriminating between T and B lymphocytes. Furthermore, it reveals the potential for the reversible localization of SLC25A41 to demonstrate its distinct activity. This study is the first report to unveil a novel strategy of SLC by exporting the probe. We anticipate that this research will open up new avenues for developing fluorescent probes. |
| first_indexed | 2024-04-24T17:55:36Z |
| format | Article |
| id | doaj.art-eb7bfc7015a04c2187a1aaa0ca7853f9 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-24T17:55:36Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-eb7bfc7015a04c2187a1aaa0ca7853f92024-03-27T13:59:52ZengMDPI AGPharmaceutics1999-49232024-03-0116342410.3390/pharmaceutics16030424Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport SystemHeewon Cho0Na-Kyeong Hong1Young-Tae Chang2Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Gyeongsangbuk-do, Republic of KoreaDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Gyeongsangbuk-do, Republic of KoreaDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Gyeongsangbuk-do, Republic of KoreaFluorescent bioprobes are invaluable tools for visualizing live cells and deciphering complex biological processes by targeting intracellular biomarkers without disrupting cellular functions. In addition to protein-binding concepts, fluorescent probes utilize various mechanisms, including membrane, metabolism, and gating-oriented strategies. This study introduces a novel fluorescent mechanism distinct from existing ways. Here, we developed a B cell selective probe, CDrB, with unique transport mechanisms. Through SLC-CRISPRa screening, we identified two transporters, SLCO1B3 and SLC25A41, by sorting out populations exhibiting higher and lower fluorescence intensities, respectively, demonstrating contrasting activities. We confirmed that SLCO1B3, with comparable expression levels in T and B cells, facilitates the transport of CDrB into cells, while SLC25A41, overexpressed in T lymphocytes, actively exports CDrB. This observation suggests that SLC25A41 plays a crucial role in discriminating between T and B lymphocytes. Furthermore, it reveals the potential for the reversible localization of SLC25A41 to demonstrate its distinct activity. This study is the first report to unveil a novel strategy of SLC by exporting the probe. We anticipate that this research will open up new avenues for developing fluorescent probes.https://www.mdpi.com/1999-4923/16/3/424fluorescent carbohydrate libraryhigh-throughput phenotypic screeninggating-oriented live-cell distinctionsolute carrierB cell-specific probe |
| spellingShingle | Heewon Cho Na-Kyeong Hong Young-Tae Chang Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System Pharmaceutics fluorescent carbohydrate library high-throughput phenotypic screening gating-oriented live-cell distinction solute carrier B cell-specific probe |
| title | Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System |
| title_full | Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System |
| title_fullStr | Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System |
| title_full_unstemmed | Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System |
| title_short | Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System |
| title_sort | novel fluorescent strategy for discriminating t and b lymphocytes using transport system |
| topic | fluorescent carbohydrate library high-throughput phenotypic screening gating-oriented live-cell distinction solute carrier B cell-specific probe |
| url | https://www.mdpi.com/1999-4923/16/3/424 |
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